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Citations for Promega :
201 - 250 of 1126 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified BAC and plasmid DNA were transfected into HEK293 cells with FuGene HD transfection reagent (Promega) in a 1:3 DNA:FuGene HD ratio ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were transfected with β1AR and a cyclic-permuted luciferase reporter construct (pGloSensor-20F, Promega) and luminescence values were measured ...
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bioRxiv - Cell Biology 2022Quote: ... a sheep anti-BrdU antibody was used in the presence of RQ1 DNase (Promega, 10 units/ml). and after washing with conjugated anti-sheep antibodies (see antibodies section) ...
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bioRxiv - Plant Biology 2020Quote: ... Magne® Halo Tag® Beads (Promega, USA, Catalog No G728A) were used to purify recombinant proteins according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid expressing the Halo tag (pFN21A) was purchased from Promega. The expression plasmids His12_PreScission _C1_C2B_MUN_C2C_tev_Halo and His12_PreScission _C1_C2B_MUN_tev_Halo (ΔC2C Munc13 ...
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bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... reference beads followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The NanoLuciferase (NLuc) and Halo-Tag genes were obtained from Promega and the plasmids expressing b2AR- ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Luciferase activity in infected hACE2-HEK293 cells was measured with a Bright-Glo Luciferase assay system (Promega) and a Beckman Coulter DTX880 plate reader ...
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bioRxiv - Biophysics 2020Quote: ... Changes in the intracellular cAMP concentration of the HEK293 cells were measured by the GloSensor assay (Promega). The transfected cells were incubated with or without 0.5 μM all-trans-retinal (Toronto Research Chemicals) ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were washed with 1X PBS buffer and then lysed with 1X passive lysis buffer (Promega). The cells were cleared of any cell debris by centrifugation at 14000 rpm for 10 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* (67) ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... and amplified PCR products using primers listed in Table S1 were sub-cloned into pGEM-T Easy (Promega). Purified plasmid DNAs were sequenced using M13 primers.
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bioRxiv - Microbiology 2021Quote: ... Final enrichment was achieved with a nuclease digestion using DNase I and S1 nucleases (Promega Corporation, Madison, WI). Enriched dsRNA was shipped overnight on dry ice to Quick Biology (Pasadena ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removed from hTNPO1 using proTEV Plus Protease (Promega), and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with 0.5 μM Halo-tag substrate CA-AlexaFluor488 (Promega) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Halo-tag expressing cells were labelled with Halo-TMR ligand (Promega - G8251) following the manufacturers instructions for live cell labelling prior to fixation and permeabilization.
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bioRxiv - Microbiology 2022Quote: ... The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega) and verified by Western blotting with the anti-HaloTag antibody (Promega) ...
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bioRxiv - Biophysics 2022Quote: ... and a goat anti-mouse tag: HRP 0.1 μL/mL (W402B - Promega).
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bioRxiv - Biochemistry 2023Quote: ... and cells were chased with HaLo-Tag TMR ligand (5 µM, Promega) in Opti-MEM for 15 min ...
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bioRxiv - Systems Biology 2023Quote: HEK293 cells stably expressing μOR or μOR-APEX2 were transiently transfected with the cAMP biosensor pGLO-20F (Promega). Prior to agonist stimulation ...
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bioRxiv - Microbiology 2021Quote: ... Spike+ cells were then single cell sorted into 96-well PCR plates containing RNase inhibitor (N2611; Promega) and stored at −80 °C.
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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bioRxiv - Genetics 2020Quote: ... Founder mice were analyzed by PCR using primers described in Table S1 and PCR products were subcloned in pGEM.T Easy Vector for sequencing (Promega). F1 and F2 mice were confirmed by PCR and by PCR products sequencing (primers available in Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... LaMATE2ORF sequence was cloned in pGEM-T easy vector (for primers see Table S1, Promega Italia Srl, Milan, Italy) and deposited in the NCBI database (KY464927).
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bioRxiv - Genetics 2024Quote: ... Existing plasmids constructs of variant-centered test elements for the five variants of interest (see Dataset S1 for genomic regions) cloned in the pGL4.23 vector (Promega) were used as the initial template in PCR ...
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bioRxiv - Bioengineering 2019Quote: ... cells were transfected using FuGene HD transfection reagent according to their standard protocol for HEK293 cells (Promega, Madison, WI). DNA plasmids were cloned in Dam-negative E ...
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bioRxiv - Immunology 2020Quote: ... The sleeping beauty transposon system was stably transfected into HEK293 EBNA cells using FuGENE® HD (Promega GmbH, USA) in DMEM/F12 (Merck ...
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bioRxiv - Biochemistry 2023Quote: The recruitment of PTH1R to β-arrestin was detected in HEK293 cells using the NanoLuc Binary System (NanoBiT; Promega). The Lgbit subunit was fused to the C-terminus of PTH1R and the SmBiT subunit was fused to the N-terminus of β-arrestin ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293 culture medium was replaced by imaging medium containing either Janelia Fluor 646 (JF646) ligand (10 nM; Promega, #GA1120) or OregonGreen ligand (50 nM ...
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bioRxiv - Biochemistry 2023Quote: BromoTag cell lines were generated in HEK293 cells via simultaneous transfection of two vectors at a 4:1 reagent:DNA ratio with FuGENE 6 (Promega). The first vector was a pMK-RQ vector containing 500 bp homology arms on either side of either an eGFP-IRES-BromoTag or eGFP-IRES-HiBiT-BromoTag sequence for integration into MCM4 and BRD4 ...
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bioRxiv - Neuroscience 2020Quote: ... and inserting epitope tags via polymerase chain reaction with Pfu DNA polymerase (Promega). Primers for HA insertion into pIRES2- EGFP-FL ...
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bioRxiv - Synthetic Biology 2021Quote: ... We also included N-terminal and C-terminal HiBiT tags (Promega, Madison, WI) for quantification using luminescence ...
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bioRxiv - Developmental Biology 2021Quote: ... the GST tag was cleaved from GST-TES-1 using ProTEV Plus (Promega), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... the cells were incubated with 1 nM Halo Tag TMR Ligand (G825A, Promega) at 37 °C for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... and pMRXIH together with 3×FLAG tag or HaloTag7 (N2701, Promega, Madison, WI). DNA fragments encoding ATG2A (NM_015104.3 ...
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bioRxiv - Neuroscience 2023Quote: ... and dyes used were anti-Halo-Tag (Promega, cat. G928A, 1:200 dilution), FluoTag-X2-anti-PSD95-Sulfo-Cy5 (28)(NanoTag Biotechnologies GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR products of the Strep-Flag-tag and NLuc (Promega, cat # N1091) were inserted into the pHLmMBP-8 vector (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... were co-transfected with 0.5 μg of spike expression plasmids in pCDNA3 using Fugene 6 and following the manufacturer’s instructions (Promega). Cell-cell fusion was measured using an Incucyte and determined as the proportion of green area to total phase area ...
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bioRxiv - Cancer Biology 2023Quote: A volume of 250 μl fraction was mixed with 500 pg of luciferase RNA spike-in (Promega #L4561) and lysed in 750 μl Trizol LS (Thermo Fisher #10296028) ...
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bioRxiv - Immunology 2022Quote: ... into 293T cells in DMEM medium + 10% FCS using Fugene 6 (Promega) for pseudoviruses production ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (qPCR) was performed with the primers listed in Table S1 and a GoTaq qPCR Master Mix (Promega, A6002) in a Mastercycler realplex2 thermocycler (Eppendorf) ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: The upstream regions of genes of interest were amplified by PCR with specific primers (Table S1) and cloned into the pGEM-T easy plasmid (Promega). After digestion with proper restriction enzymes (Table S1) ...