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Citations for Promega :
201 - 250 of 298 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in musclebioRxiv - Cell Biology 2022Quote: ... modified with 10mM Iodoacetamide in 100 mM ABC (at the dark, RT, 30 mins) and digested in 10% Acetonitrile and 10 mM ABC with modified trypsin (Promega) at a 1:10 enzyme-tosubstrate ratio overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 40% confluent HAP 1 WT cells (seeded in a 10-cm dish) were co-transfected with the two plasmids described above (5 µg each) using 30 µl Fugene6 transfection reagent (Promega) in OptiMEM medium ...
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bioRxiv - Biophysics 2021Quote: ... Samples were incubated at 25°C for 30 minutes with gentle shaking before addition of 50 µL Kinase GloTM Max reagent (Promega) and further incubation at 25°C for 10 minutes ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μL of lysate was diluted with 30 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Systems Biology 2021Quote: ... The reaction was stopped after 30 minutes by 100-fold dilution into PBS and the amount of nanoluciferase was measured using Nano-Glo (Promega) on a Centro XS3 luminometer (Berthold).
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 30 minutes in the dark before being read at 405 nm on a GloMax Explorer (Promega).
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Molecular Biology 2021Quote: ... the culture supernatant was removed and cells were lysed by incubation for 30 min at room temperature with Cell Culture Lysis Reagent (Promega). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... the cell culture medium was removed and cells were incubated for 30 min with 1x concentrated Cell Culture Lysis Reagent (Promega), before cell lysates were transferred into white opaque-walled 96-well plates and luminescence was recorded (1 sec/sample ...
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bioRxiv - Microbiology 2020Quote: ... alkylated with iodoacetamide (IA) 55 mM in BCA 50mM (30 min) and enzymatically digested with Trypsin Gold Mass Spectrometry Grade (Promega) for 10 h at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and dried in at 37 °C before overnight digestion with 30 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then incubated for 30 min on ice in the presence of 10 μL of 20 ng/μL trypsin (Trypsin V5280, Promega), followed by 16 h at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: Phage selections were done according to previously established protocols.30 Selections were performed using biotinylated antigens captured with streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were diluted and aliquoted to 96-well plates to make less than 1 cell count per well and lysed by 30 μl of Passive Lysis Buffer (Promega) when confluent ...
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bioRxiv - Microbiology 2020Quote: ... coli was grown in NB liquid medium (180 rpm, at 30 ° C) and had its DNA extracted with Wizard Genomic DNA Purification Kit (Promega). The number of bacteria in the seed and root was calculated based on the values of Ct (cycle threshold ...
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bioRxiv - Molecular Biology 2021Quote: ... the culture supernatant was removed and cells were lysed by incubation for 30 min at room temperature with Cell Culture Lysis Reagent (Promega). Next ...
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bioRxiv - Microbiology 2020Quote: ... protein lanes were cut into 10 pieces, destained (600 rpm, 37 °C, 200 mM NH4HCO3 in 30% acetonitrile) and digested with trypsin (sequencing grade, Promega) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... coli was grown in NB liquid medium (180 rpm, at 30 °C) and had its DNA extracted with Wizard Genomic DNA Purification Kit (Promega). The reference DNA was diluted in series of 10−2 to 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Neuroscience 2021Quote: ... Trpc4 and Trpc5 mRNAs was performed on 3 µl of cDNA from single cells in a 30 µl reaction volume containing 1X GoTaq Flexi buffer (Promega), 2 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μg of psPAX2 packing plasmid and 2 μg of pM2G envelope plasmid were transfected into HEK293 Lenti-X cells using 30 μL of Fugene-HD (Promega) in antibiotic-free DMEM ...
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bioRxiv - Immunology 2022Quote: ... plates were equilibrated at room temperature for at least 30 minutes before 100 µL of 1:1 diluted PBS and CellTiter Glo reagent (Promega) was added to 100 µL of media ...
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bioRxiv - Synthetic Biology 2024Quote: ... Four microliters of CFPS sample were added to 30 μL of ONE-Glo Luciferase Assay System (Promega, Madison WI, USA) in a white 96-well plate (Costar 3693 ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from 30 mg of ground plant tissue using the SV Total RNA Isolation System Kit (Promega) and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... cells were infected at MOI 1 for 24 h and cells were frozen dry at -80°C for 30 min and lysed in Passive Lysis Buffer (Promega). Bioluminescence was measured using the Nano-Glo® Luciferase Assay System (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were incubated for 1.5 hr at 30 °C and luciferase signal was measured using the Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Physiology 2024Quote: ... Cysteine residues were alkylated in presence of 20 mM iodoacetamide at 37 °C in the dark for 30 min and tryptic digestion (sequencing grade, Promega) was performed at a 100:1 protein to enzyme ration at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Microbiology 2023Quote: ... cells grown on white 96-well plates with clear bottoms were transfected with a polio replicon RNA expressing the Renilla luciferase gene in the complete medium supplemented with 30 μM of cell-permeable Renilla luciferase substrate Enduren (Promega). The plates were sealed with a transparent oversheet and incubated directly in the heated chamber of a multifunctional M3 microplate reader (Molecular Devices) ...
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bioRxiv - Microbiology 2022Quote: ... and alkylated with 5 mM of iodoacetamide 30 min in the dark at RT°C prior to digestion with 5 μg of trypsin Gold (Promega) for 15 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing 20-30 pmol rhodopsin were mixed with 100-250 fmol BSA and cleaved with 1 μg trypsin/LysC mix (Promega) using the SP3 beads protocol described in [29].
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus was generated by transfecting the Plat-E packaging cell line with 10 µg plasmid DNA and 30 µl FuGene HD transfection reagent (Promega). Viral supernatants were collected from transfected Plat-E cells at 40 ...
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bioRxiv - Biochemistry 2022Quote: ... The transfected neurons were stimulated with 200μM glycine for 3 min and then washed for 30 minutes before lysis in 100 μl of buffer for dual luciferase assay (Promega). 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax ...
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bioRxiv - Biochemistry 2023Quote: ... cells were starved by 50 μL Hank’s balanced salt solution for 30 min and then incubated in 50 μL CO2-independent media containing 2% GloSensor cAMP Reagent (Promega) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... the plates were allowed to equilibrate to room temperature for 30 minutes and 25 μL of Cytotox Glo solution (Promega) was added to the wells using a Wellmate plate filler ...
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bioRxiv - Microbiology 2023Quote: ... diluted with 40 μL PBS and assayed for secreted gaussia Luciferase (gLuc) activity by injecting 30 μL coelenterazine solution (Promega) followed by 1.6 sec incubation period and a luminescence integration read time of 1.0 sec ...
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bioRxiv - Microbiology 2023Quote: ... and then reverse transcribed into complementary DNA (cDNA) using 30 enzyme units(U) of the MMLV-RT (Promega, WI, USA) and 1μM of the labeled primer ...
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bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Biochemistry 2023Quote: ... for 30 min at 37□C (For NVX-CoV2373, 9 different conditions were used viz., 16 h of trypsin (Promega) digestion ...
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bioRxiv - Genomics 2024Quote: ... with one half treated with DNase (briefly, incubation at 37°C for 30 min after adding 10 units of RQ1 RNase-free DNase, Promega) and one half not treated with DNase (considered non-DNase-treated virome samples) ...
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bioRxiv - Cancer Biology 2024Quote: ... 70 ng of a firefly luciferase reporter construct containing XPA sequences and 30 ng of the Renilla luciferase control (pRL-TK; Promega) were mixed with 0.2 µl of Jetprime transfection reagent in 10 µl of Jetprime transfection buffer (Polyplus ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of cell lysates were diluted 1:30 and assessed for firefly and Renilla luciferase activity using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Microbiology 2024Quote: ... Samples were amplified from 30 ng of extracted DNA in triplicate 15 µl reactions using Go-Taq DNA polymerase (Promega) with the addition of 3.3 μg bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from half a volume of total RNA (1 μg) treated for 30 min at 37°C with RQ1 RNase-free DNase (Promega) using a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μL IVT reactions were carried out for 2 hours at 30°C using commercially available Nuclease-Treated Rabbit Reticulocyte Lysate (RRL, Promega) and reaction components were added in the following order ...
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bioRxiv - Biochemistry 2024Quote: ... was then incubated with nucleosome substrate (300 nM) for 30 minutes at room temperature following addition of 500 µM ATP (Promega) in a reaction volume of 20 µL (in 50 mM Tris-Cl pH 7.6 ...