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Citations for Promega :
151 - 200 of 298 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... were extracted from 30 eggs and treated with DNase I using ReliaPrep RNA Tissue Miniprep System (Promega). cDNAs were synthesised from the purified RNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... cells were lysed by the addition of 30 μL of passive lysis buffer (Promega, Madison, WI, USA.) and three freeze-thaw cycles ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated with 5 mM iodoacetamide for 30 min in the dark and digested overnight with trypsin (Promega; 1 μg for cell-surface-enrichment samples ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Biochemistry 2022Quote: ... after which the dsDNA template was degraded for 30 minutes with 15 units of RQ1 DNAse (Promega). The transcribed sgRNA was PAGE purified on an 8% denaturing polyacrylamide gel containing 7 M urea ...
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bioRxiv - Neuroscience 2021Quote: DNase treatment was then performed (RQ1 10x buffer, RQ1 DNase by Promega, 30 minutes at 37°C) and stopped by adding 1 μL of DNase Stop Solution (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were analyzed after 30 h incubation for luciferase reporter activity with DualGlo luciferin reagent (Promega) using a luminometer (Thermo Fluoroskan Ascent FL) ...
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bioRxiv - Genomics 2021Quote: ... DNA was resuspended in 30 uL PCR grade water and quantified by Quantus™ Fluorometer (Promega Inc). The PCR product pools were sequenced by short read sequencing as described by 2.3.1.
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bioRxiv - Immunology 2023Quote: ... Cells were concentrated in 30 µL of 1X PBS + 0.2 U RNasin® Plus Ribonuclease Inhibitor (Promega), from which 5 µL were used for measuring viability and cell numbers by trypan blue exclusion assay ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a 30 µL reaction volume containing 1X Colorless GoTaq Reaction Buffer (Promega, #M792B), 200 µM dNTPs (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was carefully collected and loaded onto 30 µl MagneHis™ beads (Protein Purification System, Promega) and incubated for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... at 37°C for 30 minutes prior to the incubation with 2µl RQ1 DNase stop solution (Promega) at 65°C for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with a total of 10 μg plasmid DNA using 30 μL FuGENE HD (Promega). The following day ...
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bioRxiv - Cell Biology 2020Quote: ... room temperature for 30 min) and digested in 10 % acetonitrile and 10mM ammonium bicarbonate with modified trypsin (Promega) at a 1:10 enzyme-to-substrate ratio ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 20–30 mg of liver tissue with the Maxwell SimplyRNA Tissue Kit (Promega) on a Maxwell RSC 16 instrument (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were collected by centrifugation at 8000 × g for 30 s or using a magnet separator (Promega), and washed three times with lysis buffer ...
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bioRxiv - Synthetic Biology 2021Quote: Protoplasts were flash frozen in liquid nitrogen and lysed by adding 30 μl of Passive Lysis Buffer (Promega). NanoLuc activity was then measured using the Nano‐Glo® Luciferase Assay (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were diluted to 1.5 M urea using 20 mM HEPES (pH 8.5) and digested at 30°C overnight with trypsin (Promega) at a ratio of 70:1 (w/w) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferation was measured every 24h by treating cells with 30 μL of the CellTiter Glo kit reagent (Promega) and measuring luminescence with a CLARIOstar Plus microplate reader (BMG Labtech).
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bioRxiv - Microbiology 2023Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 µl of DNAse (Promega) and 2.5 µl of RNAse (Macherey-Nagel ...
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bioRxiv - Biochemistry 2024Quote: ... 30 µg of proteins from lysed cells were digested with 500 units of endoglycosidase H (ENDO H, Promega) and 10 units of Peptide N-glycosidase F (PNGase F ...
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bioRxiv - Microbiology 2022Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 μL of DNAse (Promega) and 2.5μL of RNAse (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with the supernatant (total protein amount of 20-30 μg) supplemented with 6.4 mM MgCl2 and 0.5 U/μl Recombinant RNase Inhibitor (Promega) in total volume of 30 μl ...
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bioRxiv - Cell Biology 2022Quote: ... room temperature for 30 min) and digested in 10% acetonitrile and 10mM ammonium bicarbonate with modified trypsin (Promega) at a 1:10 enzyme-to-substrate ratio ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin digestion was performed by addition of 30 µL of 10 ng/µL sequencing grade trypsin (Promega, Madison, WI) in AmBic followed by 30 µL of additional AmBic ...
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bioRxiv - Molecular Biology 2021Quote: ... the RNA/oligonucleotide mixture was diluted to 30 µl final with a reaction mix (1X RNase H reaction buffer, 65 µM DTT, 0.5U/µl RNasin (Promega), 50 U RNase H (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... before cells were permeabilized using 0.2% Triton X-100 PBS for 10 min at room temperature, then blocked for 30 min (2% BSA / 0.5% fish skin gelatine / PBST.5, 2U/µL RNAsin Plus (Promega) at room temperature) ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Immunology 2022Quote: ... 300 ng of this plasmid together with 30 ng of a renilla luciferase control plasmid (pGL4.74[hRluc/TK], Promega) were transfected in keratinocytes (24 wells ...
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bioRxiv - Cell Biology 2022Quote: ... For phospho-peptide samples the volume was increased to 2,000 μl (7-fold dilution) and 30 μg Trypsin Gold (Promega) was added to each sample ...
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bioRxiv - Microbiology 2022Quote: ... Automated luminescence (Annexin V) and fluorescence (membrane integrity) measurements were collected every 30 mins in a GloMax Explorer (Promega) held at 37°C.
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at 30° C in the dark and finally tryptically digested with 1.25 µg of sequencing grade porcine trypsin (Promega) in 100 mM ammonium bicarbonate at 37° C for 16 h ...
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bioRxiv - Physiology 2022Quote: ... Cells were then air-dried for 30 min and lyse with 200 μg/ml proteinase K (Promega, WI, USA) in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and the cells were lysed with 30 µl/well of 1× cell lysis buffer (Promega) for 15 min ...
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bioRxiv - Biophysics 2020Quote: 10 μg of α-syn PFFs in PBS (PH 7.4) were incubated for 30 min at 37 ℃ in the presence of trypsin (Promega) or proteinase K (Invitrogen) ...
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bioRxiv - Synthetic Biology 2020Quote: ... LucN luminescence was detected from the same sample by adding 30 μL NanoDLR™ Stop & Glo® Reagent (Promega). After incubation for 10 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were then incubated at 37 °C for 2 min before the addition of 30 units of SmaI (Promega), 46 kBq [a-32P]-dCTP (111 TBq/mmol ...
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bioRxiv - Developmental Biology 2020Quote: ... C2C12 myoblasts were seeded at 20-30% confluence in 10cm format (CELLSTAR) and forward-transfection with Fugene-6 (Promega) according to manufacturer’s protocol with the following plasmids ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated for 30 min at 4 °C slowing rotating with Passive Lysis Buffer (Promega Cat#E1941). After incubation with the lysis buffers ...
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: ... We used lipofectamine 2000 to transfect 106 NIH-3T3 MEFs with 3 μg of the luciferase plasmid with a WT DBS or its mutant counterpart and 30 ng of a renilla luciferase expression plasmid (pGL4.73, Promega) for normalization ...
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bioRxiv - Cancer Biology 2023Quote: ... UK) and were used within 30 passages of purchase or subsequent authentication by: STR profiling (PowerPlex Fusion System, Promega). C33A and SiHa cells were grown in Dulbecco’s Modified Eagle Medium (DMEM;) ...
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bioRxiv - Immunology 2023Quote: ... Bacterial growth was followed for 24h or 18h with OD600nm measurements every 30 min using GloMax Plate Reader (Promega). Anaerobia was maintained with a O2 less sealing film (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Media was removed by spinning the inverted plates at 150g for 30 seconds followed by addition of BrightGlo (Promega) for quantification of P ...
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bioRxiv - Synthetic Biology 2023Quote: ... LucN luminescence was detected from the same sample by adding 30 μL NanoDLR™ Stop & Glo® Reagent (Promega). After incubation for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 170 µL medium was removed and 30 µL caspase 3/7 reaction (1:100 Z-DEVD-R110 fluorogenic substrate:Homogenous buffer; ApoONE, Promega) was added to each well ...
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bioRxiv - Cancer Biology 2024Quote: ... The dye was released from cell inserts using 30% acetic acid and absorbance was measured using GloMax Explorer (Promega) microplate reader at 590 nm as a readout for cell migration.
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bioRxiv - Biochemistry 2024Quote: ... The translation mixtures were incubated at 30°C for 90 mins followed by the dual-luciferase reporter assay (Promega). The 2μl translation products were added into a white 384-well microplate (PerkinElmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... The 96-well plate was then allowed to equilibrate at room temperature for 30 minutes followed by addition of Cell Titer Glo (Promega) to each well according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... Proteins were digested using Lys-C (Wako, 1:40, w/w, overnight under gentle shaking at 30°C) and modified trypsin (Promega, 1:60 ...