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Citations for Promega :
201 - 250 of 263 citations for InP ZnS PEG NH2 Quantum Dots 640 nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR reactions with final primer concentration of 500 nM were prepared using GoTaq qPCR master mix (Promega A6001) in 20 µl reactions and run on a Viia-7 (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... 48 h after transfection culture media was changed to contain 100 nM tetramethylrhodamine (TMR)-conjugated Halo ligand (Promega #G8251). After 20 min incubation ...
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bioRxiv - Cell Biology 2024Quote: ... the cells expressing H3.2-HaloTag or CDC45-HaloTag were fluorescently labeled with 50 nM HaloTag TMR ligand (G8252; Promega) or JF646 ligand (GA1120 ...
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bioRxiv - Cell Biology 2022Quote: ... coated with carbon and 0.1% gelatin as described previously (Maeda et al., 2020) and treated with 20 nM HaloTag SaraFluor 650T ligand (GCKA308; Promega) for 15 min before fixation ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 30 minutes in the dark before being read at 405 nm on a GloMax Explorer (Promega).
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bioRxiv - Cell Biology 2021Quote: ... The first round of selection was performed manually using 300 nM of biotinylated K29-linked diubiquitin immobilized onto 250 µL of Streptavidin Paramagnetic Particles (Promega). Following washing with Selection Buffer (SB ...
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bioRxiv - Cancer Biology 2021Quote: ... lactate assays were performed with culture media collected from each sample according to the manufacturer’s protocol and the optical density was measured at 450 nm using a microplate reader (GloMax®-Multi E7031, Promega)
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL in vitro translation reactions were performed in the linear range using 2.8 nM mRNA in the Flexi RRL System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Genomics 2020Quote: ... HCT116 Tet-OsTIR1 Scc1-mAID-Halo cells were fluorescently tagged by incubating with 50 nM HaloTag diAcFAM (Promega, cat# G8272) or 500 nM HaloTag JF488 for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega). The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured at 550 to 570 nm wavelength and followed by 100 μl of 1X Stop & Glo Reagent (part of the kit E1910, Promega) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected cells were then treated with Aβ42 (1-4 μM) or calcitriol (100 nM) for 6 h before luciferase activity assay (Dual-Luciferase Reporter Assay, Promega). A 587-bp region of the human Cyp24a1 gene promoter that contains two VDRE motifs was cloned into the promoter-less luciferase expression vector pGL3-basic (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected six hours later using a Synergy 4 instrument (BioTek) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1 h at 37 °C with fresh complete DMEM containing JFX646 HaloTag ligand (200 nM; GA112A; Promega), washed twice with PBS 1X and imaged on an Elyra 7 with Lattice SIM² microscope (Zeiss ...
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: β-arrestin2 recruitment to human chemokine receptors in response to 1 µM VUF15485 or 200 nM positive control chemokines was also monitored by NanoLuc complementation assay (NanoBiT, Promega) (Dixon et al. ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensity of each well and empty controls was measured at 520 nm excitation on a Glomax plate reader (Promega). The average blank control values for each plate were subtracted from individual fluorescence intensity values and weekly measurements were normalized to the initial reading.
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The slides were exposed to 365 nm UV light and the damaged BrdU/BrdC-substituted DNA strands were subsequently digested by 800 U Exonuclease III (Promega) in the dedicated buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Amounts of protein binding to M.tb were quantified by colorimetric measurements by absorbance at 450 nm using a GloMax plate reader (Version 3.0; Promega Corporation), and plotted based on absorbance values relative to the baseline (negative control ...
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bioRxiv - Neuroscience 2023Quote: ... Fifty microliters of stopping solution was added to each well and the absorbance at 490 nm was read on a GloMax (Promega).
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bioRxiv - Bioengineering 2023Quote: Cells’ viability cultured on control ITO and NM devices was examined with CellTiter-Glo Viability Assay (CTG, Promega, Mannheim, Germany). After sterilization of the devices with 70% ethanol followed by UV irradiation for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 24 h later the cells were incubated with a final concentration of 16 nM Halo-Ligand JF 549 (Promega, GA1110) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... samples transferred to a fresh plate containing an equal volume of CETSA detection reagent – assay buffer supplemented with 100 nM of purified LgBiT-His protein and 0.5X furimazine (Nano-Glo® luciferase assay system, Promega). Samples were then incubated at room temperature for 30 minutes before transfer to a 384-well white plate (Greiner-One ...
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bioRxiv - Biochemistry 2024Quote: ... Purified Haspin (465-498; 250 nM) was incubated for 1 hour at room temperature following addition of 1 mM ATP (Promega) in a reaction volume of 50 µL (in 50 mM Tris-Cl pH 7.6 ...
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bioRxiv - Cell Biology 2024Quote: PDZD8-HaloTag knocked-in HeLa cells were incubated with 200 nM Janelia Fluor (JF) 549 dye (Promega, catalog no. GA1110) at 37°C o/n ...
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bioRxiv - Cell Biology 2024Quote: Correlative light and electron microscopy was conducted as previously described.61 PDZD8-Halotag knocked-in HeLa cells were overexpressed with plasmids encoding Venus-FKBP8N403K or YFP-ActA and then treated with 200 nM Janelia Fluor 549 dye (Promega) for 20 hours at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Clones expressing the desired fusion gene were determined first by staining with 100 nM JF549-HTL (Cat. No. GA1110, Promega) and 50 nM Hoechst 33342 and identifying clones with the expected distribution of JF549 signal ...
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bioRxiv - Molecular Biology 2024Quote: ... The absorbance at 450 nm for each well was measured using a Promega GloMax luminescent detector (Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2024Quote: ... The absorbance of each well at 450 nm was measured using a Promega GloMax luminescence detector (Promega, Madison, WI, USA).
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bioRxiv - Biochemistry 2024Quote: ... was then incubated with nucleosome substrate (300 nM) for 30 minutes at room temperature following addition of 500 µM ATP (Promega) in a reaction volume of 20 µL (in 50 mM Tris-Cl pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were treated with either 500 nM HaloProtac3 (HP3; a gift from the Adelman lab or purchased from Promega, GA3110) or DMSO/Ent-HP3 (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... dye was withdrawn from stained colonies with 10% acetic acid and the associated absorbance was measured using a microplate reader at 600 nm (GloMax Explorer system, Promega)(45).
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Cell Biology 2020Quote: ... at a concentration of 40 nM or with 1500 ng of pcDNA3.1-DR5-AS with the Fugene HD transfection reagent (Promega, United States) in a 2-mL final volume ...
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bioRxiv - Neuroscience 2021Quote: The remodeling of neuron isolated mitochondria was evaluated according to a published protocol,[54] by measuring the changes in the absorbance of the mitochondrial suspensions at 540 nm using a GloMax Discover multimode plate reader (Promega, Italy). Briefly ...
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bioRxiv - Physiology 2024Quote: ... The optical density of the standards and samples was recorded at 450 nm wavelength using a microplate reader (Promega, GloMax 9301). All samples were run in triplicates.
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was evaluated by measuring either luminescence or absorbance (490 nm) with the CellTiter-Glo® luminescent cell viability assay (Promega) or the CellTiter (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... In Ghent the optical density was measured by diluting the culture in phosphate-buffered saline in a 96-well microtiter plate and measuring the turbidity in a plate reader at 600 nm (GloMax® Explorer Multimode Microplate Reader, Promega).
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bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
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bioRxiv - Cell Biology 2023Quote: ... incubated 3 h at 37°C and alkaline phosphatase (SEAP) activity determined measuring the absorbance at 600 nm using GloMax®-Multi Detection System (Promega).
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bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Biochemistry 2024Quote: ... The well plates were incubated at 30 °C and the optical density at 560 nm was measured using a plate reader (Promega ProMax). We defined a growth inhibition of at least 50 % in comparison to the growth in the DMSO-supplemented medium as the cut-off to determine the MIC.
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bioRxiv - Cell Biology 2020Quote: ... A total of 250 µL of BMB was added to each well and the optical density of the samples was read using a Glomax plate reader set at 525 nm (Promega, Madison, USA). The amount of GAG in each sample is expressed as a percentage of the dry weight of the digested ECM.
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bioRxiv - Biochemistry 2020Quote: ... or PACAP (10 nM final) and assayed 3 hours later with the Nano-Glo® Dual-Luciferase® Reporter System (Cat# N1630; Promega) on a Berthold Sirius luminometer to quantify ERK activation.
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bioRxiv - Immunology 2024Quote: ... AP20187 dimerizer was added in cell culture media to a final concentration of 500 nM for 5 hours before taking the supernatants for LDH assay (G1780, Promega, Madison, WI). Cell death was measured on a scale with the media without cells set to 0% and cells lysed for 1 hour in 1% Triton X-100 set to 100%.
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega, Dübendorf, Switzerland).
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bioRxiv - Biophysics 2023Quote: HeLa cells expressing each Halo-tagged chromatin remodeler were labeled with 5 nM Janelia Fluor® 549 (JF549) Halo-tag® ligand (GA1110, Promega) for 15 min at 37 °C ...