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Citations for Promega :
201 - 250 of 2513 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... before cells were permeabilized using 0.2% Triton X-100 PBS for 10 min at room temperature, then blocked for 30 min (2% BSA / 0.5% fish skin gelatine / PBST.5, 2U/µL RNAsin Plus (Promega) at room temperature) ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Biochemistry 2024Quote: ... immune precipitated samples were washed 5 times with lysis buffer and treated with Trypsin Gold (2 μg/ml; Promega) in 20 mM Tris-HCl (pH7.5 ...
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bioRxiv - Physiology 2024Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... followed by a reverse transcription using a universal degenerated oligo specific to all 5′ non-coding sequences of BTV-1 segments (BTV/Uni1; 5′ GTTAAAWHDB 3′) and the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA). The cDNAs obtained for the segment 7 were then quantified with a real time quantitative PCR (qPCR) ...
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bioRxiv - Immunology 2021Quote: ... CDC was measured after incubation for 3 hours at 37 °C 5% CO2 with a luminometer using the CytoTox-Glo Cytotoxicity Assay (Promega; Cat. Nr.: G9291) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
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bioRxiv - Molecular Biology 2024Quote: ... 16.5 μg attB-plasmid library + 3 μg pCAG-NLS-Bxb1 + 2 μg pMAX-GFP + 2150 μL serum-free DMEM + 80 μL FugeneHD (Promega cat# E2311), then incubated at room temperature for 15 min and added dropwise to cells ...
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bioRxiv - Developmental Biology 2024Quote: ... tRFValCAC sequence was inserted into the multiple-cloning site at the 3’ end of the hRluc gene within the psiCHECK-2 Vector (Promega, cat # C8021). mESCs were transfected using the method described above ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
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bioRxiv - Cancer Biology 2021Quote: ... Caspase-Glo 3/7 Reagent (Caspase-Glo® 3/7 Assay, Promega) was added to the wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was detected using Caspase-Glo 3/7 (Promega). Annexin V/Propidium Iodide staining was performed using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... caspase-3/7 levels were examined using Caspase-Glo 3/7 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Biochemistry 2022Quote: ... 2ml of Sf9 cells at 0.5×106 cells per ml were transfected with 2 µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a ratio of 3:1 transfection reagent to DNA ...
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bioRxiv - Molecular Biology 2021Quote: A total of 10 μg of bacmid DNA was transfected into 6 wells of 2×106 adherent Sf9 cells (at 5×105 cells/ml) using the transfection reagent Fugene HD (Promega). 42–72 h post transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... elution buffer I (2 M Urea, 50 mM Tris pH 7.5, 1 mM DTT and 5 ng/µl Trypsin (Promega, #V5111)) were added and samples were incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... and NanoLuc fused to the amino terminal 112 amino acids of human Phosducin circularly permutated at amino acids 54/55 (Promega). The NanoLuc/Phosducin fusion portion also contains a kRAS membrane targeting sequence at the carboxy terminal end ...
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bioRxiv - Microbiology 2021Quote: ... or 3) Pepsin (Promega). 1 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL RNasin (Promega) and 100 µL lysis buffer for a total volume of 300 µL for IP by rotation for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 0.24 mM amino acid mix – met (Promega L9961), 10.2 mM DTT) ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.05 mM each of 20 amino acids (Promega), 0.5 mM spermidine ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.05 mM each of 20 amino acids (Promega), 0.5 mM spermidine ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.6 μl complete amino acid mixture (Promega, L4461), 0.4 μl Ribolock nuclease inhibitor (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μM amino acid mixture without leucine (Promega), and 1 U/ml ScriptGuard RNase inhibitor (CELLSCRIPT)] ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 μM amino acid mixture minus leucine (Promega), and 0.5 U/μl SUPERase•In RNase Inhibitor (Thermo Fisher Scientific)] and incubated for 1 h at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... 6 μL S30 premix without amino acids (Promega), plus water to a final reaction volume of 15 μL were incubated for 1 h at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... stained with Diamond nucleic acid dye (H1181, Promega), and visualized using excitation light at ∼500 nm ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using 5 μl of the eluent as template using the U3 antisense primer 5’ TGTGTGGTAGATCCACATCG-3’ and M-MLV RT RNase [H-] (Promega, WI. Cat. No. MR3681) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... the pHAD plasmid was linearized by PmeI enzyme to expose the ends of the 5’ and 3’ inverted terminal repeats (ITRs) and transfected into 116 producer cells (Profection® Mammalian Transfection System, Promega, Madison, WI, USA). Helper virus (HV ...
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bioRxiv - Immunology 2020Quote: Activity of the inflammatory caspases 1/4/5 was measured using a commercially available Caspase-Glo® 1 Inflammasome Assay (Promega, WI, USA) from HFFs seeded in 96-well plates (2×104 cells per well) ...
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bioRxiv - Biochemistry 2021Quote: ... treated with 3 mM dithiothreitol (DTT) (molecular grade, Cat. 3483-12-3, Promega), and incubated for 30 min at 37°C in 5% CO2 in serum-free RPMI media ...
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bioRxiv - Microbiology 2022Quote: ... the virus-induced cytopathogenic effect was measured colorimetrically by the formazan-based 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI), and the antiviral activity was expressed as the 50% effective concentration (EC50) ...
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bioRxiv - Genetics 2023Quote: ... 3,500,000 cells were seeded in 10 cm plates (2-4 per replicate) and transfected with FuGENE® 6 Transfection Reagent (Promega, E2692). In one tube ...
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bioRxiv - Synthetic Biology 2022Quote: To measure luciferase activity on plaque containing LB-agar plates, 5 μl of the diluted (1:50, in PBS) NanoLuc® luciferase substrate 2-furyl methyl-deoxy-coelenterazine (Furimazine; Promega) were dropped onto the respective plaques ...
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bioRxiv - Microbiology 2022Quote: Various KSHV mRNA 5’-UTRs containing uORFs were cloned up stream of the Renilla luciferase gene in a psiCHECK™-2 vector (Promega) using a Gibson Assembly® Cloning Kit (New England Biolabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 48 hours (Figure 2) of drug exposure at 37°C/5% CO2 and assayed for cell viability using CellTiter-Glo® (Promega) by following manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: ... HiBiT intensity was measured daily in three biological replicates (3 measurements x 3 wells x 3 replicates / day) in 384-well format using a HiBiT quantification kit (Promega Nano-Glo). 5µl HiBiT reaction mixture (1:100 LgBiT protein and 1:50 HiBiT lytic substrate in HiBiT lytic buffer ...
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bioRxiv - Immunology 2020Quote: ... After a migration time of 4 h at 37°C (5 % CO2) lower compartment were analyzed for cell content by Cell-Titer-Glo® assay (Promega, Madison, WI, USA). Percentages of migrated cells were calculated as described before45 and normalized to the CXCL12-only control.
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bioRxiv - Cell Biology 2020Quote: ... and 10 μM methionine-free amino acid mixture (Promega). The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μM amino acid mix w/o methionine (Promega)) ...
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bioRxiv - Neuroscience 2023Quote: ... and stained in Diamond Nucleic Acid Stain (Promega, H1181). All experiments were performed in 8-12 week old animals ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase-3 and -7 activity was measured by Caspase-Glo 3/7 Assay (Promega) according the manufacturer’s instructions using a BioTek Synergy H1 plate reader.
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bioRxiv - Cancer Biology 2021Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega) and caspase activity was normalized to cell number by performing the CellTiter-Glo Luminescent Cell Viability Assay on the duplicate plate.
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega), and caspase activity was normalized to cell number by performing the CellTiter Glo Luminescent Cell Viability Assay on the duplicate plate ...
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bioRxiv - Cancer Biology 2022Quote: ... caspase-3 and -7 activities were measured by Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s instructions.