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Citations for Promega :
2401 - 2450 of 2857 citations for Codanin 1 CDAN1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... mRNA was isolated from ∼1 mg total RNA using oligo(dT) and streptavidin paramagnetic beads (PolyATtract mRNA Isolation Systems, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... 2 μl US bait primer and 2 μl Illumina universal primer 10 μM (0,4 μM final concentration each primer) and 1 μl GoTaq (Promega, M5005) with the following program ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using 1:100 (w/w) LysC (Wako Chemicals) for 4 h at 37°C and then finalised with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Microbiology 2020Quote: ... 4 µl of the lysates were mixed with 20 µl of Renilla Luciferase Assay Buffer and 1/200 of substrate from the Renilla Luciferase assay system (Promega) and measured immediately in a luminometer for 2 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were diluted and aliquoted to 96-well plates to make less than 1 cell count per well and lysed by 30 μl of Passive Lysis Buffer (Promega) when confluent ...
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bioRxiv - Molecular Biology 2020Quote: The AP-1 luciferase reporter was expressed using an adenoviral vector and luciferase activity determined using a luciferase assay kit from Promega as previously described (Higazi et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were checked on a 1% agarose gel and were purified using the Wizard PCR clean-up kit (Promega). DNA concentration and purity were measured using a ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies).
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bioRxiv - Synthetic Biology 2021Quote: ... were generated by in vitro transcription from DNA templates generated by PCR amplification or by fill-in with primer T7X (Supplementary Table 1) using GoTaq (Promega). DNA was purified using QiaQuick (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR assays were performed using 1 ng of cDNA as template and the reactions were conducted using the GoTaq qPCR Master Mix (Promega). Real-time RT-PCR was performed on a MasterCycler Ep-Realplex thermal cycler (Eppendorf ...
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bioRxiv - Microbiology 2019Quote: ... In-gel protein digestion was performed by O/N incubation of the gel pieces with 1 µg of sequencing grade modified trypsin (Promega) in 67 mM ammonium bicarbonate pH 8 at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5mM of dNTPs ...
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bioRxiv - Microbiology 2021Quote: ... The cytotoxicity of compounds (100 uM to 1 uM, 3-fold dilution) was examined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cell cytotoxicity data was normalized to DMSO control as 0% cell death.
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bioRxiv - Molecular Biology 2020Quote: ... Plates were incubated at room temperature for 1 hour before luminescence was measured using a GloMAX Discover Plate reader luminometer (Promega). Three replicate experiments were performed with three biological replicates for each cell type and treatment within each experiment.
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bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
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bioRxiv - Molecular Biology 2020Quote: ... Human NOCT (1-431) or Schistosoma japonicum GST coding sequences were inserted into pFC3F using the Flexi Cloning System (Promega). To generate NOCT Δ(2-15)-3F ...
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bioRxiv - Microbiology 2020Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified by means of Wizard SV gel and PCR Clean Up kits (Promega). The amplified gene was treated with Taq polymerase (Fermentas ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 50 μL (fLuc assays) or 100 μL (NanoLuc assays) of 1× Cell Culture Lysis Reagent (Luciferase Assay System; Promega) and incubated for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Genomics 2019Quote: ... PCR products run on 1% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up System (Promega). Purified PCR products were sequenced and analyzed with the Sequence Scanner Software 2 Version 2 (Applied Biosystems).
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bioRxiv - Biophysics 2019Quote: ... as well as pre-activated β-arrestin2 harboring 3A mutations (residues 1-393, I386A, V387A and F388A) (Kang et al., 2015) were inserted into a NanoBiT PPI plasmid (Promega). The large subunit (LgBiT ...
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bioRxiv - Developmental Biology 2019Quote: ... The University of Queensland) constructs were co-transfected (at ratio of 1:10) with renilla luciferase (pRL-TK vector, PROMEGA) in Non Targeting Control or H2a.z.2 siRNA treated B16 melanoma cells ...
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bioRxiv - Cancer Biology 2019Quote: ... Stable cell lines were generated by co-transfecting pOG44 with pCDNA5/FRT/TO vectors encoding BRCA1 tBRCT (aa 1620-1862) in a 9:1 ratio using FuGENE HD transfection reagent (E2311, Promega). After 10 days of selection using Blasticidin (15μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on an Infinite M200PRO plate reader (TECAN ...
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bioRxiv - Genetics 2020Quote: ... these primers were also designed with restriction enzyme site overhangs (BamHI and HindIII) to facilitate cloning into the pGEM-1 plasmid (Promega) (Fig ...
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bioRxiv - Genetics 2020Quote: ... The primers were designed with restriction enzyme site overhangs (BamHI and HindIII) to facilitate cloning into the pGEM-1 plasmid (Promega) (Fig ...
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bioRxiv - Genomics 2020Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... as described previously(40) and 1 μg of RNA was reverse transcribed to cDNA using the ImPromIITM Reverse Transcriptase kit (Promega), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... v/v) and dehydration in ACN, proteins were digested overnight at 37 °C with trypsin (1:50, w/w) (V5280, Promega). Peptides were extracted from the gel in 50% ACN/0.1% formic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Mixtures were incubated for 1 hour at room temperature before fluorescence readings were taken using a GloMax Explorer (Promega, WI) plate reader with an excitation filter at 405 nm and emission filter at 500 – 550 nm ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were then washed twice as described above and submitted to overnight proteolysis in ammonium bicarbonate buffer containing 1 μg of trypsin (Promega) per sample at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μL of a solution that is 1 mM in each of the 20 essential amino acids (Promega, No. L4461); 20 μl of Promega S30 Premix without Amino Acids (No ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were digested either with trypsin in a trypsin/protein ratio of 1/50 (w/w) (Promega Cat. No. V5111) and incubated overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell pellet was suspended in buffer P1/RNAse A and lysed by adding 1/20 volume of 10% SDS (Promega). After incubating for 20 min at room temperature ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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bioRxiv - Biophysics 2021Quote: ... were plated into six-well tissue culture plates containing collagen-coated glass cover slips 12 to 24 h before being transfected with 1 μg of the indicated plasmid DNA using FuGENE 6 transfection reagent (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Cell Biology 2020Quote: ... The reduced and alkylated protein lysates were diluted using 50 mM Tris-Cl to obtain a final concentration of 2 M urea in solution followed by overnight digestion with trypsin (1:1000)(Promega). Peptides were then desalted using c-18 Sep-Pack columns (Waters) ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed in 1 x PBS and cell viability was assessed using CellTiter-Glo Luminescent Cell Viability Assay (Promega). Luminescence read-out was normalized to signal from vehicle treated cells and represented as percentage of viable cells compared to vehicle ...
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bioRxiv - Bioengineering 2021Quote: ... knees were injected with 50 μL furimazine (1:50 dilution in PBS, Nano-Glo™, PRN1120, Promega, Madison, WI, USA), then assessed with the above IVIS settings ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested day 1 and day 5 post-infection using a CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Plates were incubated at 25 °C for 1 hr and fluorescence was measured using a BioTek Synergy Neo plate reader (Promega). The terbium (Tb ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...