Labshake search
Citations for Promega :
2251 - 2300 of 2857 citations for Codanin 1 CDAN1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Trypsin digestion was performed using a trypsin solution with a concentration of 1 μg /μL (Trypsin Gold, V528A, Promega), and 0.6 μL of the trypsin solution was added to achieve a final ratio of 1:50 ...
-
bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
-
bioRxiv - Biochemistry 2023Quote: Cells stably expressing epitope-tagged NK1R were transiently transfected (Lipofectamine™ 3000 Transfection Reagent) with 1 μg of pGL4.29[luc2P/CRE/Hygro] or pGL4.30[luc2P/NFAT-RE/Hygro] plasmids (Promega Corp.), seeded in a white-walled 96-well flat clear bottom plate (Corning #3610) ...
-
bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck], 1 unit/mL RNasin Ribonuclease Inhibitor [N2515, Promega]) and frozen at -80 °C.
-
bioRxiv - Immunology 2023Quote: Transient transfection was performed using 1 or 2 µg plasmid DNA and 3.5 or 7 µl FuGENE HD transfection reagent (Promega) per 5x 105 cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... protoplasts were transferred onto BCD-growth medium containing ammonium tartrate and geneticin G418 antibiotic (50 µg ml−1; Promega) followed by two weeks growth period without the antibiotic and second selection period for two weeks with antibiotic again.
-
bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
-
bioRxiv - Biochemistry 2023Quote: BromoTag cell lines were generated in HEK293 cells via simultaneous transfection of two vectors at a 4:1 reagent:DNA ratio with FuGENE 6 (Promega). The first vector was a pMK-RQ vector containing 500 bp homology arms on either side of either an eGFP-IRES-BromoTag or eGFP-IRES-HiBiT-BromoTag sequence for integration into MCM4 and BRD4 ...
-
bioRxiv - Developmental Biology 2024Quote: ... containing the promoter region of spia-1 (1.23 kb upstream from the start ATG) and the entire genomic region into the pGEMT vector (Promega). A transcriptional construct (pSO22) ...
-
bioRxiv - Plant Biology 2024Quote: ... The alkylation was stopped by the addition of 1 µl of 200 mM cysteine and the samples digested overnight with 1 µl of 0.5 µg/μl trypsin (MS Gold, Promega). The resulting peptides were desalted and subsequently analysed on UltiMate™ 3000 RSLCnano System connected to LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific).
-
bioRxiv - Cancer Biology 2024Quote: ... Cell content was measured on Days 1-6 using Cell Titer-Glo Luminescent Cell Viability Assay (Cat# G7570, Promega). Luminescence was measured at an integration time of 0.5 second per well ...
-
bioRxiv - Immunology 2024Quote: ... plates were washed 5 times with wash buffer (PBS with 1% BSA (Capricorn Scientific) and 0.05% Tween-20 (Promega)) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Transfection solutions were made at a lipid (µg) to plasmid (µL) ratio of 3:1 using FuGene 6 (Promega) as the transfection reagent ...
-
bioRxiv - Cell Biology 2024Quote: ... reduced (6.5mM DTT, 1 hour at 60°C), alkylated (54mM Iodoacetamide, 30min, RT) and digested with 1µg of Trypsin (Promega) (96) ...
-
bioRxiv - Genetics 2024Quote: ... YFP-negative first instar larvae of the progeny of gwnull- 1/PBac(681.P.FSVS-1)PMCACPTI001995 or gwnul1-2/PBac(681.P.FSVS-1)PMCACPTI001995 were collected 38 hr after egg laying and were lysed in Passive lysis buffer (Promega). After centrifugation ...
-
bioRxiv - Microbiology 2024Quote: ... At 8 and 24 hpt monolayers were lysed with 100 μl 1 x passive lysis buffer according to the manufacturer’s instructions (Promega), stored at −80°C and analysed using Dual-luciferase substrate (Promega ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were stained with 1 nM Halo Tag Ligand (HTL) tagged with Janelia Fluor 646 (HTL-JF646, Promega, #GA1110) for 30 min at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
-
bioRxiv - Cancer Biology 2024Quote: Purified rRNA (1 to 3 µg) was digested overnight at 37 °C with 270 units of Nuclease S1 (Promega) using the supplied buffer ...
-
bioRxiv - Biochemistry 2020Quote: 2.5 μg of bacmid DNA per construct obtained from amplification in DH10BacY cells were used to transfect 1 Mil Sf9 cells/per construct using Fugene transfection reagent (FuGENE HD, Promega). Virus at P0 was harvested and used to produce a stock Virus P1 solution to further infect 1 lt culture of Sf9 cells at 0.8-1 Mil/ml in SF921 medium containing Pen/Strep ...
-
bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 10 μL of isolated RNA with 1 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested overnight at 37 °C with 5 μL of trypsin (1 μg dissolved in 50 mM HEPES pH 8.0, Promega V5111). The trypsin digestion was quenched by adding 4 μL of 1× EDTA-free protease inhibitor cocktail (Roche 11873580001) ...
-
bioRxiv - Cell Biology 2020Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). Luminescence was measured with a SpectraMax Gemini XPS luminometer (Molecular Devices) ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized by reverse transcription from 1 μg total RNA using a ImProm-II™ Reverse Transcription System (Promega). Primers for NMNAT1 ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on a Victor 3 plate reader (Perkin Elmer) ...
-
bioRxiv - Developmental Biology 2021Quote: ... HEK-293T cells were co-transfected with corresponding pLKO.1 plamids and 3rd generation packaging lentiviral plasmids (pLP1, pLP2, pLP-VSVG) using FuGENE HD (Promega), according to the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2021Quote: ... the proximal promoter region (−299/−1) of MyoG was isolated by PCR and inserted into the pGL3-basic vector (Promega). The DNA fragments of PME1 and PME2 in the MyoG promoter region were also amplified and inserted into the pGL3-γ-actin-TATA vector ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were incubated for 6 h at 37°C before being harvested in 1× Passive Lysis Buffer and subjected to a Dual-Luciferase Reporter Assay System as per manufacturer’s instructions (Promega, E1980) on a GloMax-Multi plate reader (Promega) ...
-
bioRxiv - Developmental Biology 2020Quote: ... neural stem / progenitor cells were seeded in 96-well coated plates (1×104 cells per well) and RealTime-GloTM MT Cell Viability Assay (Promega) was used with the continuous-read protocol at 0 ...
-
bioRxiv - Microbiology 2020Quote: ... DNA fragments of approximately the correct size were extracted from 1% agarose gels and ligated into the pGEMT-Easy (Promega) subcloning vector ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 10 μg of each protein was then boiled in PCR tubes at 100 °C for 5 min and allowed to cool to room temperature before 1 μg mass spectrometry-grade trypsin (Trypsin Gold from Promega) was added to each sample ...
-
bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
-
bioRxiv - Neuroscience 2022Quote: ... Imaging was done in a perfusion chamber with artificial cerebral spinal fluid (ACSF) as described in36 using 1 μM furimazine (Promega), heated to 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... Proteins were digested using Lys-C (Wako, 1:40, w/w, overnight under gentle shaking at 30°C) and modified trypsin (Promega, 1:60 ...
-
bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
-
bioRxiv - Molecular Biology 2021Quote: ... cells were co-transfected with 2 µg of pWH464 (pSpCas9(BB)−2A-GFP (pX458)) expressing the corresponding targeting sgRNA and 1 µg of the corresponding donor oligo or plasmid using the Fugene6 (Promega). To generate miR-182Δ and miR-183Δ mESCs ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL RRV-P3 plasmid or 3 μL of cDNA from rose tissue or nuclease-free water (Promega, Madison, WI). The final volume of the LAMP reaction was 25 μL.
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were digested with 0.5 µg of lysyl endopeptidase (Wako) at room temperature for 4 hours and were further digested overnight with 1 µg trypsin (Promega) at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated at 37°C for 1 hour and then proteolytic digestion was performed with LysC (Wako) for 4 hours and trypsin (Promega) overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from cells at the exponential phase (OD600 ~1) with the acidic hot phenol method 41 and treated with DNase (Promega). 50 μg of total cellular RNA was incubated with 1 μM of the corresponding DNAzyme ((Supplementary Table S2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
-
bioRxiv - Cell Biology 2022Quote: ... coated with carbon and 0.1% gelatin as described previously (Maeda et al., 2020) and treated with 20 nM HaloTag SaraFluor 650T ligand (GCKA308; Promega) for 15 min before fixation ...
-
bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
-
bioRxiv - Molecular Biology 2021Quote: ... Liberibacter OmpA was amplified using SgfI and PmeI restriction sites (Table 1) and was digested using Flexi Enzyme Blend (Promega). Similarly ...
-
bioRxiv - Molecular Biology 2021Quote: ... The GAL1 promoter was amplified from yeast genomic DNA using primers 4588-1/XH5103 and ligated into pGEM-Teasy (Promega) to create pCB4554 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega) were finally added and the reverse transcription was performed at 35°C for 2 minutes ...
-
bioRxiv - Systems Biology 2020Quote: ... to a final concentration of 1% DOC before overnight digestion at 37 °C with lysyl endopeptidase (Wako Chemicals) and sequencing-grade porcine trypsin (Promega) at an enzyme-substrate ratio of 1:100 for both ...
-
bioRxiv - Microbiology 2019Quote: ... Total RNA concentration was quantified using a Nanodrop and 1 μg of total RNA was treated with RQ1 DNase (Promega) at 37°C for 45 mins ...