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Citations for Promega :
2401 - 2450 of 4906 citations for 7 8 Dimethoxy 2 3 4 5 tetrahydro 1H benzo e 1 4 diazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 5 mL of overnight cultures using the Wizard Genomic DNA Purification Kit (Promega Corp., Madison, WI, USA) and DNA concentration was measured using a Qubit™ Fluorometer and Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Bright-Glo reagent was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega, USA), with titers given in Relative Light Units (RLU ...
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bioRxiv - Microbiology 2021Quote: ... for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega, E1501).
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bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Microbiology 2020Quote: ... Amplifications were performed in a 50 µl final volume using 5 µl of DNA template and 2.5 units of GoTaq polymerase (Promega, Madison, WI). Final concentrations of MgCl2 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... miR159 and U6 RNA (see Table S3) were 5’ end-labelled with [γ-32P]ATP using T4 polynucleotide kinase (PNK) (Promega).
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was again removed and replenished with 30 mL phenol-red free Opti-MEM supplemented with 5 mM CT-TAMRA (Promega, G8251), except for no CT-TAMRA control wells ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were vigorously shaken at 80 rpm for 5 min prior to adding 75 µl of CellTiter-Glo 3D Reagent (Promega #G968B) to each well ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted 5 folds with distilled water and used in a reaction mix composed of 1x GoTaq qRT-PCR master mix (Promega, USA) and 10 nM primers for RT-qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... All PCR amplifications were assembled on ice in 25 μl reactions using 5 μl of 5X Green GoTaq buffer (Promega M3001), 0.5-1 μl of each primer (10 μM) ...
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bioRxiv - Microbiology 2022Quote: ... the PCR was performed in a total volume of 25 μl containing 5 X GoTaq® buffer (Promega, Madison, WI, USA), 2.5 mM MgCl2 ...
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bioRxiv - Microbiology 2024Quote: ... The activity of in vitro synthesized luciferase was assessed using 5 μL of the substrate from the Steady-Glo Luciferase Assay System (Promega, US). The signal was detected on plate reader Victor X5 2030 (Perkin Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated for 5 minutes with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system ...
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bioRxiv - Immunology 2023Quote: ... Plates were incubated for 22 hours at 37 °C/5 % CO2 prior to addition of 33ul Bio-Glo reagent (Promega G7940), then luminescence was read on a Clariostar plate reader.
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bioRxiv - Cell Biology 2023Quote: ... reduced with 5 mM DTT and alkylated with 20 mM acrylamide.23 The reaction was quenched by addition of 5 mM DTT and 1.5 µg of trypsin (Promega, Madison, WI) was added to the sample ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Biophysics 2023Quote: DNA dT70 was diluted to ∼100 pM in buffer (50 mM Tris-acetate pH 7.7, 5 mM Mg-acetate, 50 mM Na-acetate) and SSB (Promega Corporation, USA) was supplemented in specified concentrations (Tab ...
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bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... a calibration curve for SAH was constructed in two independent experiments using from 0 to 5 μM SAH according to the manufacturer’s protocol of the MTase-Glo kit (Promega, Wisconsin, USA). The signal was detected using a Tecan Spark microplate reader (Tecan Japan Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Excess iodoacetamide was quenched by addition of 5 mM DTT and samples were then digested by trypsin digestion (Sequencing Grade Modified Trypsin, Promega Corp) at 37°C for 6 hours in a Thermomixer with shaking (1000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken horizontally at 500 RPM for 5 min before addition of 25 µL of ready-to-use Nano-Glo substrate (Promega; N1130) for measuring luciferase intensity.
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bioRxiv - Immunology 2023Quote: The potential duck RNF135 promoter was obtained by amplifying 1577 bp 5’ of the ATG start codon from the gDNA of duck that was cloned into pGEM vector (Promega, USA) and analyzed by Sanger sequencing ...
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bioRxiv - Plant Biology 2024Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were cultured at 37C 5% CO2 with 2i + LIF medium containing 200nM of Janelia Fluor 549 HaloTag ligand (Promega GA1110). After 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by serially diluting virus stocks on 293T cells in the presence of 1 µM oseltamivir and 2.5 µg/ml amphotericin B and measuring luciferase signal 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610), as described previously80.
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bioRxiv - Cell Biology 2020Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using random hexamers and MMLV reverse transcriptase (Promega). The cDNA was used as a template for quantitative PCR performed using Syber Green reagent (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 μl of f/2 and 10X concentrated substrate (10 mM firefly luciferin, 1,000X dilution of NanoLuc Dual-Glo reagent, Promega) were added to the respective wells.
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bioRxiv - Cancer Biology 2019Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Bioengineering 2020Quote: ... the Nbs-virus mixture was removed from Vero E6 cells followed by 500 μL of DMEM with 2% FBS and 0.9% carboxymethyl cellulose (Promega) was overlaid ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was inserted into the multiple cloning site (MCS) of the psicheck-2 vector (supplied by Promega) downstream of the Renilla luciferase gene according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... alkylated using 50 mM iodoacetamide for 2 hrs at ambient temperature and then digested with 1.2 μg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol (27) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were diluted to a final concentration of 500,000 cells/mL in Expi293 expression medium plus 2% (v/v) Glosensor reagent (Promega) and incubated for 75 minutes at room temperature with gentle rotation ...
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bioRxiv - Microbiology 2022Quote: ... Samples were digested by adding 100 µL trypsin solution (10 mM TEAB, pH 7.5, 2 µg Promega trypsin #V5111) and incubated overnight at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... while the complementary sequence was cloned downstream of the Renilla luciferase reporter cassette in a psiCHECK-2 plasmid (Promega). The same target sequence was also cloned downstream of a TagRFP reporter cassette in an ePB-BSD-TT piggyback vector (see plasmids ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana leaves were harvested 2-days after infection and then applied to a Dual-Luciferase Reporter Assay System (Promega) to detect the activation of the ACS2 and ACS2mut promoters by NOR ...
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bioRxiv - Neuroscience 2020Quote: ... The first strand cDNA was synthesized from 2 μg RNA with GoScript™ Reverse Transcriptase (Promega, Madison, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... and a total amount of 2 μg RNA was reverse transcribed with a GO Script Reverse Transcriptase kit (Promega) using random primers ...
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bioRxiv - Cell Biology 2022Quote: ... 2 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...