Labshake search
Citations for Promega :
2351 - 2400 of 4906 citations for 7 8 Dimethoxy 2 3 4 5 tetrahydro 1H benzo e 1 4 diazepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: RNA was extracted after 2 days of culture with the ReliaPrep™ RNA Tissue Miniprep System (Promega, Z6111) following manufacturer’s protocol for non-fibrous tissue by adding RNA lysis buffer on pelleted cells ...
-
bioRxiv - Genetics 2020Quote: ... cells were changed into GloSensor equilibration medium (10% fetal bovine serum and 2% GloSensor assay reagent (Promega E1290) in CO2-independent medium (Gibco 18045-088) ...
-
bioRxiv - Biochemistry 2019Quote: ... In-column digest was performed by adding 25 µL of a solution containing 2 µg trypsin (Promega, V5111) in 50 mM TEAB pH 8.0 ...
-
bioRxiv - Immunology 2019Quote: ... NGP conditional medium was collected 2 days after transfection of NGP to 293T cells by Fugene HD (Promega). 10 ng/ml of LPS was mixed with conditional medium for 30 minutes and then treated to THP-1 cells.
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2021Quote: ... containing 2μl of lysis buffer (1.9ml of 0.2% v/v Triton-X l00 v/v + 0.1ml of RNasin Plus RNase inhibitor (10,000 U/ml, Promega). Smart-seq2 protocol was performed as described in Picelli et al [43] ...
-
bioRxiv - Genetics 2020Quote: ... The 10 µL Zn-finger PCR reaction was prepared containing of 2 µL of 5X reaction buffer (Promega), 0.8 µL of 2.0 mM MgCl2 ...
-
bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2022Quote: Calpain activity was measured in Caco-2 cell or organoids extracts by Calpain-Glo™ Protease Assay (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... genomic DNA was extracted from 2-wk old seedlings by using the Wizard Genomic DNA purification kit (Promega) and was subjected to PCR analysis by using a primer set consisting of a specific CaM35S promoter sequence and a gene-specific primer (Table S1) ...
-
bioRxiv - Plant Biology 2022Quote: ... 2 μg of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 μl containg 5 μL SYBR-green master mix (Roche) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation and 200 µL of supernatant was incubated for 20 min at 21°C with 5 µg of RNA in 200 µL of 2xTENT buffer (100 mM Tris pH 7.8, 2 mM EDTA, 500 mM NaCl, 0.1% Tween) supplemented with RNAsin (Promega). RNA-protein complexes were then incubated with MyOne Streptavidin T1 DynaBeads (Thermo Fisher Scientific ...
-
Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: ... Such cDNA samples were applied as templates for RT-qPCR using 2× GoTaq® qPCR Master Mix (Promega). The reactions were performed on the CFX96TM system (BIO-RAD ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were then subjected to digestion with LysC (Wako) at 37°C for 2 hours and trypsin (Promega) overnight at 37°C at a 1:50 enzyme:protein ratio ...
-
bioRxiv - Microbiology 2023Quote: ... and 2 μg of the spike SARS2 (D614G)-pCAGGS (Medicines & Healthcare Products Regulatory Agency CFAR100985) using FugeneHD (Promega) transfection reagent at a ratio of 1:3 DNA:Fugene in optiMEM (Gibco) ...
-
bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were run using 2 µL of extracted DNA in GoTaq® PCR Master Mix (Promega) and the following cycling protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were washed with 2× phosphate-buffered saline and lysed with 75 µL of passive lysis buffer (Promega), and then subjected to luciferase assay ...
-
bioRxiv - Genomics 2023Quote: ... 2×10e4 cells were plated in 96-well plates and CellTiter-Glo® Luminescent Cell Viability Assay (Promega) was used to quantify cell viability 48h after drug or vehicle treatment using a Tecan Infinite M Plex plate reader (Tecan ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... The size of any positive IS2404 detection was assessed against 2 µL of 100 bp DNA Ladder (Promega) and run at 50 V for 1.5 hrs before being visualised with an EZ Gel Documentation System (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis and qPCR were performed using the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) with an ABI Prism 7900HT Sequence Detection System (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 μg of Turbo DNase treated total RNA was incubated with or without 25 U RNase One (Promega) and 25 μg RNaseA (Thermo Scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... a 27-nucleotide spacer sequence was inserted between the BbsI and ClaI restriction sites of psiCHECK-2 (Promega) to remove the firefly luciferase gene sequence and its promoter sequence ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 μl of CellTiter- Glo 2 reagent was added to each well according to the manufacturer’s protocol (Promega).
-
bioRxiv - Genetics 2024Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
-
bioRxiv - Developmental Biology 2024Quote: ... the samples were further diluted to 2 M urea with 100 mM TEAB and trypsin (Trypsin Glod, Promega) was added at a ratio of 1:50 and the samples were incubated at 37°C over night ...
-
bioRxiv - Molecular Biology 2024Quote: ... A two-stage enzymatic digestion with 2 hr lysyl endopeptidase (FUJIFILM Wako Chemicals U.S.A. Corporation) and o/n trypsin (Promega) was then performed to generate the crosslinked peptides.
-
bioRxiv - Cell Biology 2021Quote: ... 6 μl of Trypsin/LysC solution [100 ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 75 μL (1:1 volume) of One-Glo™ reagent (E6110, Promega) was added to each well ...
-
bioRxiv - Neuroscience 2021Quote: ... IΒΑ1 (1:250, rabbit, Wako, 01919741) and p75NTR (1:1000, rabbit, G3231, Promega). Sections were washed 3×20 min in PBS prior to incubation with secondary antibodies (Alexa Fluor ...
-
bioRxiv - Biochemistry 2022Quote: ... 6 μl of Trypsin/LysC solution (100 ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm) ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were collected and mixed 1:1 with BrightGlo Luciferase assay reagent (Promega) and luminescence was measured using a luminometer (Molecular Devices ...
-
bioRxiv - Molecular Biology 2022Quote: ... alongside a 1:1 molar ratio of Luciferase (pGL4.23) to Renilla (pGL4.75, Promega) vectors ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were then diluted 1:1 with Milli-Q water and trypsin (Promega) added at the same enzyme/protein ratio ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT) or with 1 unit of RQ1 DNase (Promega; RNase-free) in 40 μL of RQ1 buffer (40 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2020Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection® Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
-
bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
-
bioRxiv - Genomics 2022Quote: ... The samples were diluted 1:1 with milliQ water and were incubated with a 1:100 w/w amount of trypsin (Promega, sequencing grade) overnight at 37 °C ...
-
bioRxiv - Molecular Biology 2020Quote: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM ...
-
bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
-
bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
-
bioRxiv - Biochemistry 2019Quote: ... Plates were then incubated at 37°C and 5% CO2 for 48 hours upon which cell viability was assessed by adding CellTiter-Glo® (Promega) to the reaction ...
-
bioRxiv - Cancer Biology 2020Quote: ... 25uL of reconstituted CellTiter-Glo Luminescent Cell Viability reagent was added to each well and incubated for 5 minutes (Promega, G7571). Luminescence readings were taken using the BioTek Synergy H1 Hybrid Reader ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
-
bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
-
bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
-
bioRxiv - Plant Biology 2020Quote: ... Individual reactions conducted with UGA-CPES consisted of a 10 µL mixture containing 5 µL of GoTaq Green Master Mix 2X (Promega Corporation), 0.5 µL of template DNA ...
-
bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...