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Citations for Promega :
2351 - 2400 of 5281 citations for 6 methyl 4 oxo N phenyl 2 3 dihydro 1 4 oxathiine 5 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Half of the lysis was treated with 5 µl of 10 mg/ml Rnase (Promega:527491) and both lystates (+/- RNase ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 before measurement of luciferase activity using Nano-Glo® Luciferase Assay (Promega, Cat # N1110) and Tecan Spark plate reader.
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bioRxiv - Immunology 2022Quote: ... Cells were then washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The PeSPL6 and PeSPL13a 5’-ends were amplified by PCR using the GoTaq Master mix (Promega), the forward 5’ Primer from GeneRacer kit and the reverse Gene Specific Primer (GSP) ...
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bioRxiv - Genomics 2023Quote: ... Samples were then allowed to cool for 5 minutes and 5μl of RNase (DNase-free; Promega) was added and incubated at 37ºC for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Digests were diluted further to a 1.5 M urea concentration and 5 µg of trypsin (Promega) was added for 6 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Genetics 2019Quote: ... total RNA was treated with 2 U of RQ1 RNase-Free DNase (Promega, USA) at 37°C for 30 min ...
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bioRxiv - Genomics 2021Quote: - A combination of KAPA HotStart ReadyMix and Pfu DNA Polymerase (2 U/μL, Promega) was used in some tests to try to increase enzyme processivity ...
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bioRxiv - Physiology 2021Quote: ... and simultaneously transfected with 2 μg of DNA using Fugene HD (catalog #E2312, Promega), accordingly to the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2 cm strips of intestinal tissues were embedded in low melting point agarose (Promega) to enrich for well-oriented crypt-villus units ...
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bioRxiv - Plant Biology 2021Quote: ... http://cshprotocols.cshlp.org/content/2009/2/pdb.prot5110.abstract) with trypsin (porcine, side chain protected; Promega). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... and the pre-cleared lysate was supplemented with 2 µl of RNasin Plus (Promega), added onto the freshly prepared ...
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bioRxiv - Biophysics 2020Quote: ... containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cAMP stock solution (Promega). The cells were then incubated for 2 hours at room temperature in the dark ...
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bioRxiv - Microbiology 2020Quote: ... Two days after transfection with 2 to 2.5 μg DNA using Fugene HD (Promega), confocal images were acquired using an FV1000 confocal laser scanning microscope (Olympus ...
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bioRxiv - Microbiology 2021Quote: ... We synthesized cDNA from 2 μL of RNA using M-MLV reverse transcriptase (Promega) and random hexamers ...
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TRANSITION OF PODOSOMES INTO ZIPPER-LIKE STRUCTURES IN MACROPHAGE-DERIVED MULTINUCLEATED GIANT CELLSbioRxiv - Cell Biology 2020Quote: ... PCR was performed with the generated cDNA and premixed 2× Taq polymerase solution (Promega) in an MJ Mini Thermal Cycler (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Luminescence was initiated by addition of a mixture of 2 μM coelenterazine-h (Promega) and 40 μM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Microbiology 2020Quote: ... RNA (2 μg) was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega). Conventional PCR was used with the primer (Table 1 ...
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bioRxiv - Immunology 2021Quote: ... F(ab′)2 fragments were generated after cleavage of IgG with IdeS protease (Promega) and then purified using TALON metal affinity resin (Takara ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with 2 μg of the respective guideRNA plasmids using FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... with 2 uM Ponasterone A overnight and incubated with JF646 HaloTag ligand (Promega, GA1120) 15 minutes before imaging ...
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bioRxiv - Plant Biology 2023Quote: ... Two (2) μg of total RNA were used for cDNA synthesis with MMLV (Promega) and random hexamers (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... or a combination of 2 μg of trypsin and 0.2 μg of LysC (Promega). Samples were incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 25 °C and digested with trypsin (Promega, 37 °C, overnight) in TEAB buffer (50 mM ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Microbiology 2023Quote: ... and TCID50 equivalents determined using a SARS-CoV-2 E gene detection kit (Promega) based upon the Berlin primer/ probe set (70).
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bioRxiv - Molecular Biology 2023Quote: ... including NCI were spiked with 2 ng of Luciferase Control RNA (LUC) (Promega, USA). These extracts were used for real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was generated from 2 µg isolated RNA using M-MLV reverse transcriptase (Promega) and diluted 1:15 for use in 12 µL qPCR reactions run on the StepOnePlus™ system (Applied Biosystem) ...
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bioRxiv - Molecular Biology 2023Quote: ... purified RNAs were additionally incubated with 2 units of RQ1-DNAse (Promega, cat# M6101) per 1 µg of RNA at 37°C for 30 min to eliminate plasmid DNA contamination ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg total RNA served as a template using M-MLV Reverse Transcriptase (Promega) and random hexamer primers following manufactureŕs protocol ...
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bioRxiv - Plant Biology 2024Quote: ... 2 mg of the extracted RNA underwent treatment with RQ1 RNase-Free DNAse (Promega) before being used for cDNA synthesis with SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 24 hours later co-transfected with 500 ng psiCHECK™-2 Vector (Promega) containing the 3’UTR of human PLIN2 gene downstream to the Renilla luciferase gene ...
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bioRxiv - Cell Biology 2024Quote: ... for 20ms with 2 pulses or lipid-mediated delivery using FuGENE HD (Promega; TM328) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega). Ligand (100 nM ...
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bioRxiv - Immunology 2021Quote: ... A 1:1 dilution of Steady Glo (Promega) and Lysis Buffer were added to the cells and incubated at RT for 15 mins ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... 1 mM CaCl2 with 1 µg trypsin (Promega). Digested proteins were acidified with TFA to pH < 2 and RapiGest SF was precipitated out ...
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bioRxiv - Cancer Biology 2021Quote: MAGI2-AS3 or FOXN3 3’UTR containing miR-452-5p wild-type (WT) or mutant (MUT) binding sites were inserted into psiCHECK vector (Promega, USA). 293T cells were transfected with the constructed luciferase plasmid together with miR-NC or miR-452-5p mimics using Lipofectamine 2000 ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were transfected with 700 ng of plasmid construct (PIB/V5_Gr9) and 3 μl of FuGENE® HD transfection reagent (Promega, USA) in 100 μl of medium per well ...
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bioRxiv - Physiology 2022Quote: A DNA fragment corresponding to partial f5 or f3a cDNAs was amplified from ovarian cDNAs by PCR using the gene-specific primers (see Supplemental Table 3 for detail) (28) and cloned into the pGEM-T Easy vector (Promega, USA). Correct sequence and orientations of inserts were validated by Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... Remaining ATP was readout after 3 h incubation using Kinase-Glo® Luminescent Kinase kit following manufacturer’s instructions (Promega, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... of miR-148a putative binding sites reporter plasmids were constructed using the circMRPS35 and 3’-UTR of STX3 sequences in the psiCHECK2 vector (Promega, USA). For IRES activity analysis ...
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bioRxiv - Cell Biology 2020Quote: Transfections were carried out with 1 μg of total DNA (500 ng for each construct or with empty expression plasmid) and 3 μl of FuGENE ® HD Transfection Reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested from Calu-3 cells and inactivated by addition of lysis buffer (Maxwell 16 viral total nucleic acid purification kit, Promega #AS1150) complemented with proteinase K ...
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bioRxiv - Microbiology 2021Quote: The cell viability of Vero E6 and Calu-3 cells was analyzed by quantification of ATP levels using the CellTiter-Glo™ Luminescent Cell Viability assay (Promega) according to the instructions of the manufacturer ...