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Citations for Promega :
2301 - 2350 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Genetics 2024Quote: ... PCR was performed using Go-Taq Green Master Mix (Promega, Madison, WI) and resulting products were resolved by 3% agarose gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... Standard PCR was performed on cDNAs using the GoTaq DNA polymerase (Promega). All primer sequences can be found in Table S23.
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bioRxiv - Immunology 2024Quote: ... RPS18 and UBE2D2 using the corresponding PCR master mix (Promega GmbH, Germany) on a CFX96 Detection System (Bio-Rad Laboratories GmbH ...
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bioRxiv - Bioengineering 2023Quote: ... PCR amplicons of ∼300 bp were amplified using a GoTaq kit (Promega), separated on a 2% agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... Purified PCR products were inserted into pGEM-T Easy vector (Promega,USA).
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bioRxiv - Cell Biology 2024Quote: ... dsRNAs were generated from PCR amplicons using a T7 RiboMAX kit (Promega). dsRNA against the bacterial kanamycine resistance gene (KAN ...
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bioRxiv - Microbiology 2023Quote: ... coli strain S17-1λpir and confirmed by colony PCR (GoTaq polymerase, Promega) and Sanger sequencing (Microsynth ...
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bioRxiv - Microbiology 2023Quote: When required PCR purified amplicons were ligated into pGEM-T Easy (Promega) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Released DNA fragments were purified by PCR product purification kit (Promega, A9282) and used to generate libraries ...
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bioRxiv - Microbiology 2023Quote: ... and the PCR-amplified fragments were ligated into pGEM-T easy (Promega). After sequence confirmation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each 50 μL amplification PCR consisted of 1 x GoTaq Buffer (Promega), 3 μM MgCl2 ...
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bioRxiv - Biochemistry 2023Quote: ... and real-time quantitative PCR experiments were performed using SYBR Green (Promega) in CFX connect Real-Time PCR detection system (Bio-Rad) ...
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bioRxiv - Biophysics 2023Quote: ... the handles were purified using a PCR clean up kit (A9282, Promega).
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bioRxiv - Genomics 2023Quote: ... PCR products were purified via gel excision and column purification (Promega, A9282), followed by insertion into the BsmBI–digested lentiV2-hU6-evopreQ1 vector by Gibson assembly (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was performed using Go-Taq qPCR Master Mix (Promega) with 2 µL of cDNA or ‘no RT’ sample in a 20 µL reaction volume using 100 or 200 nM final concentration qPCR primers ...
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bioRxiv - Microbiology 2023Quote: ... PCR was conducted using GoTaq Flexi DNA polymerase (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... a PCR fragment of the Nluc coding sequence amplified from pNL1.1 (Promega) was inserted into the XhoI and EcoRI sites of pColdI to generate pColdI-Nluc ...
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bioRxiv - Biochemistry 2022Quote: ... and the subsequent PCR product was purified using Wizard SV Minicolumns (Promega). Double-strand RNAs (dsRNAs ...
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bioRxiv - Plant Biology 2023Quote: ... and RT-PCR was performed using Go-Taq polymerase (Promega, WI, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR analysis was performed using the GoTaq Green Master Mix (Promega). Segregation primers (Supplementary Table S1 ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a total volume of 15µL using GoTaq (Promega) (94°C for 5 min then 45 cycles at 95°C for 1 min ...
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bioRxiv - Molecular Biology 2023Quote: ... both using the Wizard SV Gel and PCR Clean-up System (Promega). Inserts were incorporated into the vector via ligation reaction consisting of inserts and vector in a 5:1 molar ratio ...
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bioRxiv - Neuroscience 2024Quote: ... Patient DNA was amplified in a standard PCR reaction using GoTaq (Promega). PCR products were purified using Exonuclease I and FastAP (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Quantitative RT-PCR was performed with the GoTaq qPCR Master Mix (Promega) using 2% of cDNA in 10 μl reactions ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplified genes were purified via the Wizard PCR clean- up kit (Promega). The pRL814 (containing green fluorescent protein (GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... The subsequent PCR product was then ligated into pGEMT-Easy vector (Promega) and the presence of the mutation was validated through DNA sequencing services provided by Eton Biosciences ...
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bioRxiv - Genetics 2024Quote: ... Second round PCR products were cloned into pGEM®-T Easy (Promega) and sequenced ...
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bioRxiv - Plant Biology 2024Quote: ... the PCR product was ligated into the pGEM-T Easy vector (Promega). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... PCR amplification of cDNA samples was performed with GoTaq flexi (PROMEGA, M7806) using a distinct 8-mer barcoded oligos to distinguish the nine experimental replicates ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR fragment was subcloned into the pGEM-T vector (A1360, Promega) to generate pLM1 (for details ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplification of genomic DNA was performed using GoTaq Polymerase (Promega, USA) followed by conventional Sanger sequencing technology using a 3500xL Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... and clones were screened by PCR using GoTaq® DNA polymerase (Promega). Selected plasmids were checked by sequencing and transformed in E ...
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bioRxiv - Microbiology 2024Quote: ... Colony PCR was performed using GoTaq DNA polymerase (Promega, Madison, Wisconsin, USA) to amplify the corresponding protease genes ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR was performed using GoTaq qPCR Master Mix (Promega) at 58°C annealing temperature on a LightCycler480 instrument (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was sub-cloned into the pGEM-T vector (Promega) and confirmed by sequencing ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR products (pICOt and pICOt2) were subsequently cut with SpeI (Promega) and ligated with T4 DNA ligase (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR product was ligated into the pGEM-T Easy vector (Promega), and the orientation of the insertion was verified by sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... the edited region underwent PCR amplification using GoTaq G2 DNA polymerase (Promega) and using primers and PCR protocol for each site as listed in [9] ...
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bioRxiv - Microbiology 2021Quote: ... A 1:10 solution of sample homogenate to colentrazine was analyzed on a GloMax Explorer (Promega).
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bioRxiv - Microbiology 2020Quote: ... 85 ul of 10% SDS solution and 40 ul of Proteinase K (15mg/ml, MC500B Promega) were added and samples were incubated for 30 minutes at 60° C ...
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bioRxiv - Microbiology 2021Quote: ... in the digestion solution and lysis buffer of a Wizard SV 96 Genomic DNA kit (Promega). The samples were centrifuged for 10 min at 16,000 g and the supernatant was transferred to the binding plate ...
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bioRxiv - Bioengineering 2020Quote: ... Cell proliferation was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega Corporation) as per manufacturer’s instructions and quantified by absorbance at 490nm ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Beads were then resuspended in 50 µL trypsinization solution consisting of 10 ng/µL Trypsin (Promega) in 100 mM tetramethylammonium bicarbonate (TEAB) ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS; Promega, Madison, WI, USA) was added and incubated 1−4 hours at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with CellTiter 96® AQueous One Cell Proliferation Assay solution (Promega, Wisconsin, U.S.A.) for 2 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence.
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of supernatant was mixed with 50 µl of firefly luciferin solution (Promega, WI, USA). The bioluminescence was measured for 30 sec by Luminometer Berthold Detection System (Germany) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... S-Traps were loaded with 125 μL trypsin solution (50 ng/μL in 50mM TEAB; Promega) and incubated at 47°C for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... The gel slices were cooled on ice and a cold solution of trypsin (Promega, Madison, WI) 12.5 ng/μL ...