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Citations for Promega :
2101 - 2150 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The PCR reaction was performed with the GoTaq Green Mastermix (Promega) in a total volume of 50 µl in the presence of 0.2 µM of each oligo ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were done using 12.5 μL Master Mix 2X (Promega), 1.25 μL DMSO ...
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bioRxiv - Genetics 2020Quote: ... the PCR product were cloned into the pGEM-T vector (Promega) and sequenced with M13 reverse primer ...
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bioRxiv - Genetics 2021Quote: ... allowing us to screen F1’s by PCR (Promega GoTaq mastermix) followed by restriction digests with AflII (NEB) ...
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bioRxiv - Neuroscience 2021Quote: ... RT-PCR analyses were performed using GoTaq DNA polymerase (#M7841, Promega) in a reaction volume of 25 μl comprising 1 μl of cDNA and primer pairs specific for the 5’-end (exon 1-3 ...
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bioRxiv - Microbiology 2021Quote: ... Colony and screening PCRs were performed using GoTaq DNA polymerase (Promega; supplemented with 10% DMSO when screening B ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PCR product was digested with NotI (Promega, Cat#R6431) and XbaI (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was carried out using GoTaq Green Master Mix (Promega), following recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... and analytic PCRs were performed using MasterMix (Promega, Madison, Wisconsin, USA) or Green MasterMix (BioTools ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was cloned into a pJET1.2 cloning vector (Promega) and further subcloned into the pYES2 yeast shuttle vector between BamH1 and Xba1 sites.
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bioRxiv - Evolutionary Biology 2021Quote: ... gel-purified (Wizard SV Gel and PCR Clean-Up System, Promega) and blunted using PfuUltra HF DNA polymerase (Agilent Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were ligated into pGEM-T easy (Promega, Madison, WI), excised by restriction enzyme digest ...
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bioRxiv - Immunology 2020Quote: The PCR amplicon was cloned into a pGEMT easy vector (Promega) and then transformed into XL10-Gold ultracompetent bacteria (Agilent Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-96 real-time PCR System (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified with a purification kit (Promega, USA), and then sequenced by the dideoxy termination reaction using an AB3130 DNAxl sequencer ...
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bioRxiv - Immunology 2021Quote: ... before kit purification (SV Gel and PCR Clean up System, Promega) and ligation in presence of T4 ligase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The final PCR product was inserted into pGEM- T Easy (Promega), generating plasmid pPfRh5_C-term_LoxP ...
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bioRxiv - Microbiology 2023Quote: ... The final PCR product was inserted into pGEM-T Easy (Promega) and linearised using NcoI/SpeI and mixed with plasmid pDC2-Cas9-5’ guide1 and pDC2-Cas9-5’ guide2 before transfection ...
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bioRxiv - Microbiology 2023Quote: ... Selected transformants were screened by colony PCR using GoTaq polymerase (Promega) and the correct DNA sequences were confirmed by Sanger sequencing (Microsynth ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using GoTaq Green Master Mix (Promega, M7123). 2 µl of diluted cDNA was added to 12.5 µl GoTaq Green Master Mix and 0.4 µM of forward and reverse primers and nuclease-free water in a 25 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... The amplification was performed using GoTaq Long PCR Master Mix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each 25 μL PCR consisted of 1 x GoTaq Buffer (Promega), 3 μM MgCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplification was performed using GoTaq® Master Mix (Promega, M7122), with the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Templates for PCR were based on the SP64 vector (Promega, USA), and amplified by primers binding upstream of the SP64 promoter and roughly 200 bp downstream of the stop codon (Sharma et ...
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bioRxiv - Microbiology 2023Quote: ... Diagnostic PCR was performed using GoTaq polymerase (cat. no. M7805, Promega).
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bioRxiv - Evolutionary Biology 2023Quote: ... qRT-PCR was conducted with the GoTaq qPCR Master Mix (Promega) at a total volume of 10 µl in an ABI 7500 Fast Real Time PCR cycler (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed using GoTaq qPCR Master Mix (Promega, #A6001) with primers:
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bioRxiv - Neuroscience 2022Quote: ... Quantitative PCR (qPCR) was performed using GoTaq qPCR Master Mix (Promega). The primers used for Lrp6 were ...
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bioRxiv - Molecular Biology 2024Quote: ... This was cleaned up using a PCR cleanup kit (Promega, A9282). The biotin handle and cosmid-I95 DNA were both digested for 2 h at 37 °C with SpeI-HF (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... and 10 µL of power SYBR green PCR master Mix (Promega). The real-time qPCR was performed using a fluorescent quantitative detection system (FQD-96A ...
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bioRxiv - Microbiology 2024Quote: Real-time RT-qPCR assays (GoTaq 1-step qRT-PCR, Promega) were performed using standard fast cycling parameters (i.e ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification (Promega Wizard SV Gel and PCR clean-up system), the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification (Promega Wizard SV Gel and PCR clean-up system), the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... We performed PCR with GoTaq Hot Start Polymerase (Promega, Southampton, UK) or Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Physiology 2024Quote: ... 18 µl of PCR master mix which includes MM (Promega #xxx), 1ul of Forward/Reverse primers (10mM) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into the plasmid pGEMT easy (Promega). For each gene ...
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bioRxiv - Bioengineering 2023Quote: Yeast-colony PCR was performed using green GoTaq Master Mix (Promega) as per the manufacturer’s protocol (dx.doi.org/10.17504/protocols.io.bp2l69p95lqe/v1 ...
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bioRxiv - Biochemistry 2023Quote: ... qRT-PCRs were performed using GoTaq qPCR SYBR Green chemistry (Promega) and a Bio-Rad CFX 96 Touch System (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... with HaloTag amplified by PCR from pHTN-HaloTag CMV-neo (Promega) and mEmerald amplified by PCR from mEmerald-Sec61b-C1 (Addgene #90992) ...
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bioRxiv - Bioengineering 2024Quote: ... Quenchbody PCR products were purified using Wizard Clean up kits (Promega; as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by Q-PCR using Sybr green mix (Promega). 40 cycles were performed and relative expression of Rv0282 was determined in RS ...
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bioRxiv - Developmental Biology 2022Quote: ... from mouse genomic DNA and PCR products cloned into pGL4.23 (Promega) pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product was subcloned in pGEMT Easy (Promega, Madison WI). This construct is referred as pGEMT-Npr3 (Bae et al. ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using GoTaq® Green (Promega, Madison, WI, USA), according to the manufacturer’s protocol for RT ...
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bioRxiv - Neuroscience 2023Quote: ... qRT-PCR was performed with GoTaq qPCR Master Mix (Promega, A6002) and a Quantstudio5 Real-Time PCR Detection System (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was carried out with GoTap polymerase (Promega, Madison, WI, USA) using different pairs of primers for each gene ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification of DNA was performed using GoTaq (Promega, Cat# M7123) or Phusion (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... Quantitative PCRs were performed with GoTaq ® qPCR Master mix (Promega) with the following primer sets ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were cloned into pH6HTN His6 HaloTaq®T7 (Promega) according to the manufacturer’s instructions and transformed into E ...