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Citations for Promega :
2151 - 2189 of 2189 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 100 μL of this suspension containing 106 parasites were transferred into black clear-bottom 96-well plates and serial 2-fold dilutions were performed in triplicate adjusting the final volume to 200 μL with 300 μg/mL of beetle luciferin (Promega, France). Luciferase activity was quantified after 10 min of incubation with an IVIS Spectrum imager (PerkinElmer) ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Biochemistry 2023Quote: ... cells were washed with 1 mL of 1X PBS and incubated in 100 μL of 1X Passive Lysis Buffer (Promega E1941) at room temperature for at least 30 minutes ...
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bioRxiv - Genetics 2024Quote: ... 800 µg of total proteins were diluted with HEPES lysis buffer to a final volume of 1.2 mL and then incubated with 80 µL of 20% magnetic streptavidin beads (Promega, cat. # V7820) overnight at 4°C on a tube rotator.
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bioRxiv - Cell Biology 2024Quote: ... In order to visualise the tumour cells mice received an intraperitoneal injection of 50 μl luciferin (Promega; 5mg/ml in PBS). Isoflurane gas was used to anaesthetise mice during imaging ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by serially diluting virus stocks on 293T cells in the presence of 1 µM oseltamivir and 2.5 µg/ml amphotericin B and measuring luciferase signal 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610), as described previously80.
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml ionomycin for 6h then lysed in passive lysis buffer commercially provided in the dual luciferase reporter assay kit (Promega, no. E1910). Assay was conducted according to manufacturer’s instructions and luminescence measurements were done using CLARIOstar (BMG ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated for 8h with IFNα (1000 units/mL) then harvested and assayed for firefly and Renilla luciferase activities using the Dual Luciferase reporter assay system (Promega, cat# E1960). Relative luciferase activity was calculated by dividing the firefly luciferase values by those of the Renilla luciferase ...
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bioRxiv - Microbiology 2020Quote: ... Filters were transferred to 2 mL microfuge tubes for further processing using the Maxwell® RSC PureFood GMO and Authentication Kit (Promega Corporation) with a modified version of the kit protocol ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1.5□mL of that culture by using a Wizard genomic DNA purification kit (Promega, Madison, WI, USA), prepped using the Nextera XT kit ...
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bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
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bioRxiv - Molecular Biology 2020Quote: ... the SCN cultures were maintained in a 35-mm petri dish with 1 mL culture medium containing 0.3 mM D-luciferin (Promega, Madison, WI, USA) at 36 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Evolved strains were thawed and grown overnight in 150 ml of liquid medium and DNA was extracted using the Wizard Genomic DNA Purification Kit from Promega (WI, USA). One DNA sample (20 to 60 µg ...
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bioRxiv - Microbiology 2020Quote: ... the Wizard Genomic DNA Purification Kit was used including both lysozyme and lysostaphin (10 mg/ml each) (Ref. A1120, Promega Corporation, Spain); PacBio sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were seeded on poly-lysin coated coverslips at a concentration of 0.75 × 105 cells/ml and transiently transfected using the FuGENE® HD Transfection Reagent (Promega, Madison, WI) 28–48 h before measurement.
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bioRxiv - Microbiology 2023Quote: ... The transfected cells were treated with anthracyclines or Sendai virus (40 hemagglutinin [HA] units/ml) for 24 hours before luciferase activity was determined with the Steady-Glo luciferase assay system (Promega, cat. E2520).
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bioRxiv - Molecular Biology 2022Quote: ... Cells were treated with IFNα (1000 U/mL) for 12 h and were harvested to conduct dual-luciferase reporter assay (Promega, Madison, WI). Firefly luciferase activity values were normalized to Renilla luciferase activity ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following day cells were trypsinized and seeded in 384-well white plate (20 µl/well) in DMEM F12 (no phenol red, 4% FBS) +/-HaloTag® NanoBRET™ 618 Ligand (1 µl/ml, Promega) and +/-compounds (DMSO concentration in each sample was kept the same) ...
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bioRxiv - Bioengineering 2023Quote: Mice were administered either 200μL of 15 μg/mL D-luciferin or a 1:40 dilution of Nano-Glo substrate (Promega, diluted in DPBS) by intraperitoneal injection for firefly luciferase and Antares or nanoluciferase imaging ...
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bioRxiv - Biochemistry 2024Quote: ... In a pre-warmed solution of Reconstitution Buffer (20mM HEPES, 250mM KCl, 2mM TCEP, pH 7.5) containing 40 U/mL RNAsin (Cat#N2111, Promega, Madison, WI, USA), each Sm protein sub-complex was combined to a final concentration of 8 µM and incubated for 5 min at 37°C ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
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bioRxiv - Developmental Biology 2022Quote: Samples for CaTS-RNAseq were collected in the same way as for CaTS-ATAC but all buffers were supplemented with 1 µL/ml of RNasin Plus (Promega, Cat. No. N2615). After fixation and quenching ...
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bioRxiv - Cell Biology 2020Quote: ... washed again in DPBS and stained with 1mg/ml X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside, Promega, Madison, WI, US) in DMF (Dimetil formamide ...
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bioRxiv - Microbiology 2021Quote: ... DNA was isolated from cell pellets and culture supernatants cleared of cell debris by centrifugation of 5 min at 14000 rpm and treated with 20 U/ml DNase I (Promega, Madison, WI, USA) to remove free viral DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed 48 hr post-transfection in co-IP buffer supplemented with both protease inhibitors and RNAsin (Promega N2515, 100 units/mL). Lysates were processed and immunoprecipitations were performed as described above for the polymerase formation assay ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded on poly-lysine coated glass coverslips at a concentration of 1 × 105 cells/ml and transiently transfected using the FuGENE® HD Transfection Reagent (Promega, Madison, WI) two days before measurement.
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 100 μl of Chloroform were added in 500 ml of homogenized sample and total RNA was extracted using ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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bioRxiv - Synthetic Biology 2021Quote: ... a fairly large batch of transformed TOP10 cells was grown in 50 ml of LB and a plasmid DNA stock was isolated with the Wizard® Plus Midipreps system (Promega, catalog no. A7640). The poly(A ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with ABC to a final concentration of 1 M urea and digested using trypsin (Promega, V5113; 3 μg/mL, 37°C, 16 h). After digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of lysate was added to a 1.5 ml Eppendorf tubes containing 100 µl of luciferase assay reagent II (Promega luciferase assay system, Cat# E1500) and the mixture pipetted in and out three times ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 0.5µg of RNA from each sample were reverse-transcribed to become cDNA by mixing with the master-mix (200 u/µL M-MLV Reverse Transcriptase, Promega Cat. M170A, 1.25µM RT 5X buffer, Promega Cat. M531A, 0.5mg/mL Oligo (dT) 18 Primer ...
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bioRxiv - Microbiology 2021Quote: ... cells were resuspended at 2×106 cells/ml in culture media containing Nano-Glo® Endurazine Live Cell Substrate™ (Promega Inc, WI, USA # N2571) and DrkBiT (Promega Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... abdomens were washed with phosphate-buffered saline with 0.1% Tween (PBST) four times for 15 minutes before RNase (Promega, original concentration of 4 mg/mL) was added in a concentration of 10 μL/mL for 20 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... We added 300μl of Proteinase K Buffer 2X (200 mM Tris-HCl pH7.5; 100 mM NaCl; 20 mM EDTA; RNasin PROMEGA 20 U/μl; 0.04 mg/ml Proteinase K) and incubated for 1 h at 55 °C ...