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Citations for Promega :
1951 - 2000 of 2189 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples serving as positive controls for the cytotoxicity measurement received 72 μg/mL membrane-permeabilizer digitonin (Promega) in respective ToxGlo Media type (glucose or galactose) ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
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bioRxiv - Genetics 2023Quote: ... Additional 140 uL of lysis buffer and 40uL of proteinase K (20mg/ml, Promega, Cat. No. MC5005) were added ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 37°C with sequencing grade modified trypsin (0.01 mg/mL; Promega Corporation) to allow protein digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... mice received an intraperitoneal injection of 100 µl D-luciferin (stock of 40 mg/mL, Promega, E160). Mice were allowed to move freely for five minutes post injection prior to initiating anesthesia via isoflurane ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid purification from 50 mL bacterial cultures was made using PureYield™ Plasmid Midiprep System (Promega, A2492) for C ...
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bioRxiv - Biochemistry 2023Quote: ... The trypsin digestion commenced with the addition of 8 μL Gold Trypsin (0.5 mg/mL Promega V5280) along with 160 μL of 50 mM Tris pH 8 and 2 μL 100 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... One μg of mass spectrometry grade trypsin (Trypsin Gold, Promega; 1 mg/mL in 0.03% acetic acid) was added ...
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bioRxiv - Cell Biology 2019Quote: Proteins were incubated overnight at 37°C with 20 µl of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5µg/ml, Promega) at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... swab tips were placed into DNA/DNase/RNase-free 1.5 ml Eppendorf tubes containing nuclease-free water (Promega). The swab shafts were cut with a pair of sterile dissection scissors to allow closing of the tubes ...
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bioRxiv - Biochemistry 2019Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... converted 1µg of DNase-treated RNA to cDNA with 20nt Oligo-dT (25µg/mL; IDT) and rRNAsin (Promega). Precipitated cDNA was resuspended in water at 20ng/µL of original RNA concentration ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were digested by addition of 0.25 μg of sequencing grade trypsin dissolved in 50 mM acetic acid at 0.5 μg/mL (Promega) overnight at 37 °C with vigorous shaking ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... aliquots of the enzyme were removed from the freezer and were treated with thrombin (0.1 kU/mL, Promega) overnight at 4 °C in enzyme storage buffer (20 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysis buffer (0.1% v/v NP-40, 0.1% v/v Tween-20, 0.1mg/mL Digitonin ((Promega, Southampton, UK) in resuspension buffer ...
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bioRxiv - Plant Biology 2021Quote: ... filled with 175 μL 0.5x MS-agar medium 1% sucrose and 35μL (50 μg/mL) of Beetle Luciferin (Promega) dissolved in DMSO ...
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bioRxiv - Biochemistry 2019Quote: ... DMEM media was aspirated from the cells and 500 µL of hypotonic lysis buffer (50 mM Tris.HCl pH 7.4, 25 units/mL DNase I (Promega), 0.2 % (v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... and 200 μL of the cell/supernatant suspension was treated overnight with 80 μg/ml proteinase K (Promega) in 1x proteinase K digestion buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation through the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified through qPCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation using the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified using qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Neuroscience 2021Quote: ... gel pieces were rehydrated with 10 µL 50 mM NH4HCO3 containing 25 µg/mL trypsin (Trypsin Gold, Promega) and incubated for 15 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 2μl of lysis buffer (1.9ml of 0.2% v/v Triton-X l00 v/v + 0.1ml of RNasin Plus RNase inhibitor (10,000 U/ml, Promega). Smart-seq2 protocol was performed as described in Picelli et al [43] ...
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bioRxiv - Cell Biology 2021Quote: ... 2mL of Sf9 cells at 0.5×106 cells/mL were transfected with 2μg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a 3:1 transfection reagent to DNA ratio according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... In-gel digestion was performed by adding 25 μL of 4 μg/mL sequencing grade modified trypsin (Promega) in 25 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: ... then alkylated with 1.9 mg/mL chloroacetic acid before proteolytic digestion with 0.2 μg sequencing grade ArgC (Promega) and incubation at 37°C for 16 h ...
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bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
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bioRxiv - Cancer Biology 2024Quote: ... then washed with 1 ml of PBS and lysed using 60 μl of Cell Culture Lysis Reagent (Promega). 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then unfixed overnight in 150 mM NaCL and 60C and 50 ug/mL protease K (Promega). DNA was then extracted using a single column of QIAamp DNA Blood Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... The samples were then subjected to in-gel trypsin digest (Trypsin Gold, Mass Spectrometry Grade. Promega. 20μg/ml). Samples were loaded in an UltiMate 3000 HPLC autosampler system (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from 10 ml bacterial overnight grown cultures using Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL wash buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) ...
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bioRxiv - Microbiology 2023Quote: Mid-log phase fusobacterial cells (OD600 of ∼ 0.5) were harvested by centrifugation and combined in equal volumes (0.1 ml) with the BacTiter-Glo Reagent (Promega) in multi-well plates (Cellstar) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated for an additional hour at 55°C after adding 20 mg/ml proteinase K (Promega). DNA was extracted using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research) ...
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bioRxiv - Immunology 2024Quote: ... and cells were treated with 3µg/ml LPS O111:B4 and 0.25% (vol/vol) Fugene HD Plus (Promega) to cause transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were then diluted to 2 M urea final concentration via the addition of 20 mM HEPES pH 8.0 and digested overnight with 5 µg/ml sequence-modified trypsin (Promega) at room temperature.
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bioRxiv - Cell Biology 2022Quote: ... Beads were washed three times for 10 min with gentle rotation in high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Cell Biology 2022Quote: ... polysome were stabilised by washing cells in 20 ml ice-cold water containing 0.1 mg/ml CHX before being resuspended in 1 ml RiboTag Lysis Buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Neuroscience 2019Quote: ... Thawed tissue was minced and incubated in 1% PFA (with 1 µL mL−1 RNasin Plus (Promega, Madison, WI)) for 5 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... to 30 µL of 0.61 mM digitonin in a 1.5 mL microcentrifuge tube that had been pre-inserted in the luminometer reader (GloMax, Promega). The signal was recorded for 1 minute ...
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bioRxiv - Neuroscience 2019Quote: ... Three adult male heads were collected into a 1.5 ml Eppendorf tube and homogenized in 50 μl of Glo Lysis Buffer (E266A, Promega) at Zeitgeber time (ZT ...
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bioRxiv - Biophysics 2020Quote: ... Gel plugs were soaked in 50 mM ammonium bicarbonate (pH 7.8) containing 20 μg/mL of trypsin gold (Promega) for 24 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Single CD3-CD8-CD14-CD16-CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...