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Citations for Promega :
2151 - 2200 of 2791 citations for Mono 2 Ethyl 5 Hydroxyhexyl Phthalate 13C4 99% Dehp Metabolite Ix 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2000 in 5% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Excess iodoacetamide was quenched by addition of 5 mM DTT and samples were then digested by trypsin digestion (Sequencing Grade Modified Trypsin, Promega Corp) at 37°C for 6 hours in a Thermomixer with shaking (1000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken horizontally at 500 RPM for 5 min before addition of 25 µL of ready-to-use Nano-Glo substrate (Promega; N1130) for measuring luciferase intensity.
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bioRxiv - Plant Biology 2023Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Immunology 2023Quote: The potential duck RNF135 promoter was obtained by amplifying 1577 bp 5’ of the ATG start codon from the gDNA of duck that was cloned into pGEM vector (Promega, USA) and analyzed by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... The activity of in vitro synthesized luciferase was assessed using 5 μL of the substrate from the Steady-Glo Luciferase Assay System (Promega, US). The signal was detected on plate reader Victor X5 2030 (Perkin Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated for 5 minutes with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system ...
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bioRxiv - Biochemistry 2021Quote: ... 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK, Promega), and 40 ng ml-1 of pLEXm vector (34 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK-293 PDE4A4 cell media is further supplemented with 500 μg/ml G418 (Promega). Culture conditions were 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... which were destained and overnight-digested with trypsin (1 μg/mL, sequencing grade, Promega), before MS analysis of the peptide mixes ...
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bioRxiv - Bioengineering 2021Quote: ... The bottom of the chamber was coated with 10 μg ml−1 streptavidin (Promega) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and after adding 10 μL of 0.1 mg/mL GluC protease (Promega) incubated overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Digestion was performed by adding 10 μL of 0.2 mg/mL Trypsin Gold (Promega) and overnight incubation at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and dissociated in 50% Ham’s-12/DMEM medium containing 0.075 mg/mL liberase (Promega) for 30 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 55°C then with 200 μg/mL proteinase K (Promega) for 1 h at 55°C ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... and rehydrated in 50 mM NH4HCO3 containing 10 μg / ml trypsin (sequence grade; Promega). After incubation overnight at 37 °C peptides were extracted and collected in a new tube ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were rehydrated in 60 μL of 20 μg/mL Trypsin (Promega) in 50 mM ammonium bicarbonate solution and incubated for 2h at 55°C ...
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bioRxiv - Biophysics 2023Quote: ... grown up overnight in a 500 ml culture and maxiprepped (PureYield Maxiprep Kit, Promega). 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio ...
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bioRxiv - Microbiology 2023Quote: ... DNA samples were treated with a final concentration of 0.2mg/ml RNaseA (Promega #A7973) and 0.2mg/ml Proteinase K (NEB #P8107S ...
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bioRxiv - Immunology 2023Quote: ... droplets were resuspended in 30 ml of room temperature 6X SSC (Promega, Madison, WI) and 1 ml perfluorooctanol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were dried and rehydrated with 12.5 ng/mL trypsin (Promega, Madison, WI) solution in 25 mM ammonium bicarbonate on ice for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... We rehydrated slices with 12.5 μg/ml modified porcine trypsin (Promega, Madison, WI, USA) in 50 mM AMBIC and digested overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Mice were intraperitoneally injected with 200 μl of 40 mg/ml D-luciferin (Promega) in PBS and anesthetized with isoflurane ...
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bioRxiv - Cell Biology 2020Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using random hexamers and MMLV reverse transcriptase (Promega). The cDNA was used as a template for quantitative PCR performed using Syber Green reagent (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 μl of f/2 and 10X concentrated substrate (10 mM firefly luciferin, 1,000X dilution of NanoLuc Dual-Glo reagent, Promega) were added to the respective wells.
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bioRxiv - Cancer Biology 2019Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Genetics 2021Quote: ... 2-3 μl of the clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... parental HeLa cells were transfected with 2 μg plasmid containing a GFP cassette and 4 μl FuGENE HD (Promega) in a six-well cluster ...
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bioRxiv - Bioengineering 2020Quote: ... the Nbs-virus mixture was removed from Vero E6 cells followed by 500 μL of DMEM with 2% FBS and 0.9% carboxymethyl cellulose (Promega) was overlaid ...
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bioRxiv - Genomics 2021Quote: ... Variant or variant haplotype fragments were amplified in African American heterozygous individuals carrying the variants of interest located in the middle of the fragments (Supplementary Table 2 and 3) and cloned into the enhancer reporter vector pGL4.24 (E8421, Promega). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was inserted into the multiple cloning site (MCS) of the psicheck-2 vector (supplied by Promega) downstream of the Renilla luciferase gene according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... alkylated using 50 mM iodoacetamide for 2 hrs at ambient temperature and then digested with 1.2 μg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol (27) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Genomics 2022Quote: Cell viability upon siRNA transfection was measured through the CellTiter 96® Non-Radioactive Cell Proliferation Assay (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT)) (Promega). 1× 104 microglia cells were plated per well in a 96 well plate and transfected with either ON-TARGETplus Non-targeting Control siRNA (Horizon Discovery) ...
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bioRxiv - Bioengineering 2022Quote: ... while the complementary sequence was cloned downstream of the Renilla luciferase reporter cassette in a psiCHECK-2 plasmid (Promega). The same target sequence was also cloned downstream of a TagRFP reporter cassette in an ePB-BSD-TT piggyback vector (see plasmids ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana leaves were harvested 2-days after infection and then applied to a Dual-Luciferase Reporter Assay System (Promega) to detect the activation of the ACS2 and ACS2mut promoters by NOR ...
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bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Neuroscience 2020Quote: ... The first strand cDNA was synthesized from 2 μg RNA with GoScript™ Reverse Transcriptase (Promega, Madison, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... and a total amount of 2 μg RNA was reverse transcribed with a GO Script Reverse Transcriptase kit (Promega) using random primers ...
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bioRxiv - Cell Biology 2022Quote: ... 2 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Neuroscience 2022Quote: ... or 1.0 μg/side of MEK inhibitor 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio]butadiene (U0126; Promega, Madison, WI); 0.5 ...