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Citations for Promega :
2151 - 2200 of 2957 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Trypsin digestion was performed using a trypsin solution with a concentration of 1 μg /μL (Trypsin Gold, V528A, Promega), and 0.6 μL of the trypsin solution was added to achieve a final ratio of 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Cell Biology 2024Quote: ... reduced (6.5mM DTT, 1 hour at 60°C), alkylated (54mM Iodoacetamide, 30min, RT) and digested with 1µg of Trypsin (Promega) (96) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 1 nM Halo Tag Ligand (HTL) tagged with Janelia Fluor 646 (HTL-JF646, Promega, #GA1110) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection solutions were made at a lipid (µg) to plasmid (µL) ratio of 3:1 using FuGene 6 (Promega) as the transfection reagent ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell content was measured on Days 1-6 using Cell Titer-Glo Luminescent Cell Viability Assay (Cat# G7570, Promega). Luminescence was measured at an integration time of 0.5 second per well ...
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bioRxiv - Developmental Biology 2024Quote: ... containing the promoter region of spia-1 (1.23 kb upstream from the start ATG) and the entire genomic region into the pGEMT vector (Promega). A transcriptional construct (pSO22) ...
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bioRxiv - Plant Biology 2024Quote: ... The alkylation was stopped by the addition of 1 µl of 200 mM cysteine and the samples digested overnight with 1 µl of 0.5 µg/μl trypsin (MS Gold, Promega). The resulting peptides were desalted and subsequently analysed on UltiMate™ 3000 RSLCnano System connected to LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... plates were washed 5 times with wash buffer (PBS with 1% BSA (Capricorn Scientific) and 0.05% Tween-20 (Promega)) ...
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bioRxiv - Cancer Biology 2024Quote: Purified rRNA (1 to 3 µg) was digested overnight at 37 °C with 270 units of Nuclease S1 (Promega) using the supplied buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µL of Nano-Glo Luciferase Assay Reagent (1:50 mixture of Nano-Glo Luciferase assay substrate:Nano-Glo Luciferase assay buffer (Promega) was added to each well containing the diluted samples ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 µL and 25 µL of the trypsin solution (100 ng µL-1 of trypsin sequencing grade from Promega) were added to EV and WC samples ...
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bioRxiv - Microbiology 2024Quote: ... Each sample was treated with 1 μL of trypsin solution (0.5 μg/μL Sequencing Grade Modified Trypsin, Porcine (Promega) in 0.1 mM HCl) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were trypsinized on beads in 100 μL of digestion buffer (1 M urea, 50 mM Tris-HCl, pH 7.5, and 5 μg/mL trypsin (V5111, Promega)) ...
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bioRxiv - Microbiology 2024Quote: Viral loads were quantified as previously described [25] using the GoTaq® Probe 1-Step RT-qPCR System (Promega). The IAV primers and probe sequences are published as part of the CDC IAV detection kit using the following primers F ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein: enzyme by weight, Promega) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Digestion buffer (50 mM TEAB pH 8.0) containing sequencing-grade modified trypsin (1/25, w/w; Promega, Madison, Wisconsin) was added to the S-trap column and incubate for 2h at 47 °C ...
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bioRxiv - Immunology 2024Quote: ... and 1μg of plasmid expressing the respective spike protein using a 3:1 ratio of Fugene HD transfection agent (Promega).28,49 After 48 hours of incubation at 10% CO2 ...
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bioRxiv - Genetics 2024Quote: ... YFP-negative first instar larvae of the progeny of gwnull- 1/PBac(681.P.FSVS-1)PMCACPTI001995 or gwnul1-2/PBac(681.P.FSVS-1)PMCACPTI001995 were collected 38 hr after egg laying and were lysed in Passive lysis buffer (Promega). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... At 8 and 24 hpt monolayers were lysed with 100 μl 1 x passive lysis buffer according to the manufacturer’s instructions (Promega), stored at −80°C and analysed using Dual-luciferase substrate (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was prepared from 1 μg of total RNA using Random Primers and M-MLV Reverse Transcriptase (both Promega). Primers were allowed to bind to template RNA for 5 minutes at 70°C ...
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bioRxiv - Biochemistry 2024Quote: ... Protein digestion was done overnight at room temperature with trypsin (Promega V5113, 1/100 volume of 1mg/mL trypsin). 1% final concentration of TFA was added to quench the digestion ...
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bioRxiv - Genomics 2024Quote: In total 120k nuclei were sorted using an SH800 sorter (Sony) into 87.5μl of collection buffer (1 U/µl RNAsin (Promega, N2515), 5% fatty acid-free BSA (Proliant ...
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bioRxiv - Genetics 2024Quote: ... Membranes were probed with a 1:2,000 dilution of anti Phospho-Ser23/24 TnI antibody (Cell Signalling) and 1:6,000 diluting of anti- rabbit-HRP secondary (Promega). Loading controls following antibody stripping with Restore Plus reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was incubated for 60 minutes at 30°C and stopped by the addition of 1 reaction volume (10µL) of 5X PLB dilute to 2X in water (Promega). Firefly luciferase was then assayed on the Synergy HTX plate reader (BioTek ...
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bioRxiv - Microbiology 2024Quote: A PCR product encoding SARS Cov-2 macrodomain residues 1 to 176 was cloned into the HindIII and XhoI restriction sites of the pBIT3.1-C vector(Promega) which has an C-terminal 11 amino acid HiBiT tag.
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bioRxiv - Biochemistry 2024Quote: ... Colonies were pooled with LB and samples were diluted to an OD600 of ∼4.0 and 1 mL of concentrated cells underwent genomic DNA preparation using the Wizard Genomic DNA Purification Kit (Promega). DNA was quantified using the QuantiFluor ONE dsDNA System (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... Each library was diluted to an OD600 of ∼4.0 and 1 mL of concentrated cells underwent genomic DNA preparation using the Wizard Genomic DNA Purification Kit (Promega). DNA was quantified using the QuantiFluor ONE dsDNA System (Promega) ...
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bioRxiv - Biophysics 2024Quote: Tracking was performed in Human Bronchial Epithelial Cells (HBEC) labeled with between 0.5 pM – 1 pM JF646 dye (Promega). At this concentration of dye ...
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bioRxiv - Cancer Biology 2024Quote: PANC-1 cells were prepared as described previously (14) and ApoTox-Glo triplex assay kit (Promega, WI, USA, G6320) was used according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... was added to 0.5 mL 1% bovine serum albumin (BSA) in IP/lysis buffer (Pierce, 87788) and incubated with MagneHis beads (Promega, 32 µL suspension ...
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bioRxiv - Cancer Biology 2024Quote: ... Trypsin/LysC proteolysis was performed in 100µL 50mM ABC (pH 8) by adding Trypsin/LysC (Promega, 1:50 ratio) to precipitated EV proteins and incubated at 37°C overnight (∼20h ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were examined for viability every 4 to 6 weeks with the CellTiter-Glo assay (Promega). Cell viability was measured in quadruplicates by seeding the cells (2,000 to 3,000 per well in 96-well plate) ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were lysed at 4 hours post-transfection with 250 μL passive lysis buffer (Promega) and incubated 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were lysed at 4 hours post-transfection with 250 μL passive lysis buffer (Promega) and incubated 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and NUMB1-4 (from V.O.R.) expression plasmids was performed using FuGENE HD transfection reagent (Promega, E2311) according to the manufacturer’s protocol and selected with Geneticin (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Viability was measured every 4 days using the CellTiter-Glo luminescent cell viability assay (Promega G7573). A D300e drug dispenser (Tecan ...
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bioRxiv - Plant Biology 2022Quote: ... and GST- GBF3 were purified from 4 ml culture and immobilized on MagneGST Glutathione particles (Promega). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... then transfected with 4 μg pLZRS-GCaMP6 DNA plus 12 μl FuGENE 6 (Promega Cat. #E2691) in 800 μl of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Microbiology 2023Quote: ... then digested with 4 μL of a trypsin/Lys-C Mix (0.5 μg/uL) (Promega # V5071) using a two-step in-solution digestion overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... HRP (4 μM) was added followed by 20 μL of Nano-glo luciferase substrate (Promega, N1110) to each well ...
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bioRxiv - Biochemistry 2020Quote: 2.5 μg of bacmid DNA per construct obtained from amplification in DH10BacY cells were used to transfect 1 Mil Sf9 cells/per construct using Fugene transfection reagent (FuGENE HD, Promega). Virus at P0 was harvested and used to produce a stock Virus P1 solution to further infect 1 lt culture of Sf9 cells at 0.8-1 Mil/ml in SF921 medium containing Pen/Strep ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 10 μL of isolated RNA with 1 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested overnight at 37 °C with 5 μL of trypsin (1 μg dissolved in 50 mM HEPES pH 8.0, Promega V5111). The trypsin digestion was quenched by adding 4 μL of 1× EDTA-free protease inhibitor cocktail (Roche 11873580001) ...
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bioRxiv - Cell Biology 2020Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). Luminescence was measured with a SpectraMax Gemini XPS luminometer (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized by reverse transcription from 1 μg total RNA using a ImProm-II™ Reverse Transcription System (Promega). Primers for NMNAT1 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on a Victor 3 plate reader (Perkin Elmer) ...
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bioRxiv - Developmental Biology 2021Quote: ... HEK-293T cells were co-transfected with corresponding pLKO.1 plamids and 3rd generation packaging lentiviral plasmids (pLP1, pLP2, pLP-VSVG) using FuGENE HD (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... the proximal promoter region (−299/−1) of MyoG was isolated by PCR and inserted into the pGL3-basic vector (Promega). The DNA fragments of PME1 and PME2 in the MyoG promoter region were also amplified and inserted into the pGL3-γ-actin-TATA vector ...