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Citations for Promega :
2051 - 2100 of 2957 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies) for use with Go-Taq 1-step RT-qPCR Master Mix (Promega). Gene expression profile and SARS-CoV-2 N subgenomic RNA expression were determined with a standard qPCR cycle (50°C for 2 minutes ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:1000 dilution and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary IgG (W401B, Promega) was used in a 1:20,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted with 0.1M ammonium bicarbonate solution to a final concentration of 1% sodium deoxycholate before digestion with trypsin (Promega) at 37°C overnight (protein to trypsin ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
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bioRxiv - Bioengineering 2020Quote: ... a 632 bp fragment of the 121-promoter [15] was amplified by PCR (Suppl. Table 1) and inserted between the BglII and HindIII sites of pGL4.10 (Promega) using NEBuilder HiFi DNA Assembly Master Mix.
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bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium (Ham’s F12 with 10% FBS, Penicillin/Streptomycin, 50 mM HEPES) with 1:50 Endurazine luciferase substrate (Promega) added ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 minutes in the dark, RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, overnight at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and the cells were lysed with 30 µl/well of 1× cell lysis buffer (Promega) for 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
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bioRxiv - Immunology 2020Quote: ... The HIV-1 infectivity was evaluated by the luminescence intensity following Steady-Glo® luciferase assay (Promega; WI, USA), following company specified methodology ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then diluted 50% with PBS before sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) was added for an overnight incubation at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A) (Promega). ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Immunology 2020Quote: ... Samples were then diluted with 50 mM HEPBS pH 8.3 to reduce urea concentration down to 1 M and digested with trypsin (Promega) at 1:10 enzyme:substrate ratio overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... MI) and digested overnight in 25 mM ammonium bicarbonate (ambic) with 1:200 (w/w) dilution of trypsin (Promega) for mass spectrometric analysis ...
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bioRxiv - Cell Biology 2020Quote: ... then diluted to a final concentration of 1 M prior to in-solution digestion with 0.2 µg/µl trypsin (Sequencing grade (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed by adding 3x lysis buffer (10 mM Tris-HCl, 1.5% Igepal, 150 mM NaCl, 1 U/µl RNasin (Promega)) ...
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bioRxiv - Biochemistry 2020Quote: ... for 4 h at 37°C and then the urea concentration was reduced to 1.5 M and digested was finalized with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500 µl nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Synthetic Biology 2022Quote: ... CFPS reactions were run as described above with the addition of 1 μl of FluoroTect™ reagent (Promega L5001) per 50 μl CFPS reaction ...
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bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
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bioRxiv - Genetics 2022Quote: ... membranes were washed and incubated with the appropriate HRP conjugated secondary antibody (1:3,000 anti-rabbit W401B antibody from Promega), and the reaction was finally visualized with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega). The following primer pairs were used:
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bioRxiv - Systems Biology 2023Quote: ... Digestion was performed overnight in 300 μL of 50 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer at pH 7.5 supplemented with endoproteinase Lys-C (Wako) and trypsin (Promega) at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins captured on the columns were then digested by adding 20 μL of digestion buffer (50 mM TEAB, pH 8.5) containing 1 μg of sequencing grade trypsin (Promega) 1 hour at 47
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng of purified genomic DNA was spiked with 1 ng of unmethylated lambda DNA (Promega, Madison, WI, USA) and subjected to bisulfite treatment using the EZ DNA methylation gold kit (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged and resuspended in EZ lysis buffer, followed by three washes in wash/resuspension buffer (1x PBS, 1% BSA, 0.2U/μL RNase Inhibitor (Promega)) and labeled with propidium iodide (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times for 10 min in TBS-T and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10,000; Promega) in 5% non-fat dry milk -TBS-T for 1 h at room temp ...
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bioRxiv - Genetics 2023Quote: ... samples were suspended in homogenization buffer (50mM Tris, pH7.5, 100mM KCl, 12mM MgCl2, 1%NP40-substititue, 1mM DTT, 200U/mL Promega RNasin ...
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bioRxiv - Microbiology 2023Quote: ... and 400-800 ng of end repaired gDNA using a 1:20 dCTP:ddCTP mixture (9.5 mM dCTP Millipore Sigma #3732738001, 0.5 mM ddCTP Promega #U1225) to generate ∼20 bp C-tails.
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bioRxiv - Immunology 2023Quote: ... and digested for 4 hrs with 2 mg/ml LysC (Wako Chemicals GmbH) in a ratio of 1:50 and O/N with 0.1 µg/µl trypsin (Promega) in a ratio of 1:50 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which donor luciferase luminescence was stimulated with the addition of furimazine (1/960 dilution from Promega manufacturer’s stock) in the additional absence or presence of the orthosteric agonist 10μM isoproterenol (final assay volume ...
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bioRxiv - Neuroscience 2022Quote: ... beads were resuspended in elution buffer (50mM Tris, 1mM EDTA, 150mM NaCl, 1% SDS, 50mM NaHCO3, pH 8.1) supplemented with RNasin (Promega) and incubated with proteinase K (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of cells was then processed using the Promega Wizard® genomic DNA preparation kit (Promega, WI, USA) to obtain genomic DNA ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
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bioRxiv - Cancer Biology 2022Quote: ... Urea concentration across control and sample tubes were dropped to ∼2M with 20 mM HEPES pH 8.0 and were digested with 1 μg sequencing grade trypsin (Promega) overnight at 37°C in a nutator ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl of eluant (at 1.5 mg/ml) was treated with 6.5 µl of 1 mM HaloTag Alexa Fluor 488 Ligand (Promega PAG1001). Both labeled and unlabeled proteins were subject to size exclusion chromatography for further purification using Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: pcDNA5/FRT/TO-SFB-HELB (WT, D506G, and 3xA) plasmids (1 µg) were transfected into 1.0 × 105 cells using FuGENE HD (Promega) according to the manufacturer’s protocol followed by incubation at 37°C with 5% CO2 24 hours ...