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Citations for Promega :
2051 - 2100 of 2944 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 1 ng pEF1α-Renilla and 120 ng plasmid of interest complexed with 0.8 µl FuGENE HD (Promega) diluted to 10 µl total volume in OptiMEM ...
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bioRxiv - Biochemistry 2023Quote: ... and used 1 μL of cDNA for 10 μL of PCR using 2x GoTaq master mix (Promega) and 250 nM of forward and reverse primer sets with conditions recommended by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: Pulverized patient tissue samples were solubilized in 8M urea and 100 mM NaCl with 1% ProteaseMAX (Promega) in 100 mM ammonium bicarbonate (AmBic ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μg total RNA was used to inverse transcribe to cDNA (Promega Biotech, Madison, WI, USA), which was subsequently used as templates ...
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bioRxiv - Bioengineering 2023Quote: ... 250 ng to 1 μg of RNA were reverse transcribed using M-MLV Reverse transcrtiptase (Promega, #M1705) and oligo(dT ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... 1mM CaCl2 for overnight digestion via trypsin at 32°C (Promega, Cat# V5113; 1:50 w:w enzyme:protein). Following digestion ...
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bioRxiv - Genomics 2023Quote: ... 1 µg of Dnase-treated total RNA was reverse transcribed using the M-MLV Reverse Transcriptase (Promega) and Random Hexadeoxynucleotides (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Diluted RNA was assayed for AGO2 transcript using the GoTaq 1-Step RT-qPCR System (Promega A6020) with primers CTGGCTCCAGGGGACAAG (Forward primer ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed by incubating beads with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammbic (pH 8.3 ...
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bioRxiv - Genomics 2024Quote: ... the final selection was performed by 3% agarose electrophoresis (80 V, 2 h, 1 kB Promega ladder). The selected clones were preincubated in growth medium (15 ml ...
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bioRxiv - Cell Biology 2024Quote: ... One μg of mass spectrometry grade trypsin (Trypsin Gold, Promega; 1 mg/mL in 0.03% acetic acid) was added ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µg or total RNA was used for the cDNA synthesis (GoScript ™ Reverse Transcription System, Promega). Quantitative real-time PCRs were performed in the Agilent AriaMx System using the GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Microbiology 2024Quote: ... viral RNA was reverse transcribed and quantified using GoTaq Probe 1-Step RT-qPCR System kit (Promega) in the presence of the specific SARS-CoV-2 probe (5′-6-FAM-ACT TCC TCA AGG AAC AAC ATT GCC A-BHQ-1-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... 50ul (50.000 cells) were transferred to a new 1.5ml Eppendorf tube and 0.5ul 1% Digitonin (Promega #G9441) was added by pipetting well few times ...
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bioRxiv - Genomics 2024Quote: ... The master mixtures consisted of 1 x Taq&Go G2 Hot Start colorless PCR Master Mix (Promega), 0.5 μM of each primer ...
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bioRxiv - Systems Biology 2024Quote: ... samples were diluted 1:4 with 50 mM Tris–HCl pH 8 and sequencing grade trypsin (Promega) was added at 1:50 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol–chloroform to remove protein components ...
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bioRxiv - Plant Biology 2024Quote: ... alkylated (55 mM Iodoacetamide; 1h @ 23°C) and digested in solution with 1:100 trypsin (V5113; Promega). Peptides were acidified to 0.5% (v/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which they were treated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed on 1 μg of RNA with random hexamer primers using MMLV-RT enzyme (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sperms were released from antheridiophores by applying ddH2O supplemented with RNasin® Ribonuclease Inhibitor (1 u/μl, Promega), collected in a 1.5 mL tube ...
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bioRxiv - Pathology 2022Quote: ... A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega). Quantitative real-time PCR was performed using FastStart SYBR Green Master mix (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was subsequently performed using 1 μg total RNA and oligo-dT primers (C110A, Promega, Madison, WI). Transcript levels were assessed via real-time quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: Transfection of 1 µg of each individual DNA was performed in six-well plates using FuGENE HD (Promega) for HeLa S3 cells according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Cells were washed twice with PBS then lysed directly in wells using 1× cell culture lysis reagent (Promega). Cell lysates were clarified by centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted with three volumes digestion buffer (100 mM Tris/HCl pH 8.5, 1 mM CaCl2) and 0.25 μg Trypsin (Sequencing Grade, Promega) were added and incubated o/n at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... beads were washed two extra times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
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bioRxiv - Cancer Biology 2019Quote: ... washed two times in lysis buffer and three times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: Eluted protein samples from BIA experiment were digested in gel with 1 µg of trypsin (sequence grade, Promega) at 37°C OVN ...
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bioRxiv - Cell Biology 2019Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was reverse transcribed using 0.5–1 µg of total RNA and ImProm-II Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: The Round 1 eluted pool was PCR amplified for 10 cycles with GoTaq Hot Start Polymerase (Promega, M5001) (1X Colorless GoTaq Flexi Buffer ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 before quantitative analysis by qPCR was done using GoTaq qPCR Master Mix (Promega) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... The eluent was equally divided into three for digestion with either: 33:1 (w/w) trypsin gold (Promega), 25:1 (w/w ...
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... sample-filters were incubated in a wet-cell adding 1:50 w/w trypsin: protein ratio (Promega, V5111) solubilized in 120 µl 50 mM TEAB ...
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bioRxiv - Plant Biology 2021Quote: ... filled with 175 μL 0.5x MS-agar medium 1% sucrose and 35μL (50 μg/mL) of Beetle Luciferin (Promega) dissolved in DMSO ...
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... were transfected with 4.5 µg of the pBabe-puro-NCAM140 mApple construct (NCBI reference sequence: NM_000615.6, transcript variant 1) using Fugene 6 Transfection Reagent (Promega). Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was four-fold diluted and the protein digested in presence of 0.2 M Tris-HCl buffer pH 8.0 and 1 μg of sequencing grade trypsin (Promega) previously dissolved in 20 mM acetic acid ...
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
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bioRxiv - Neuroscience 2022Quote: ... then digested at a 1:25 enzyme:protein ratio with proteomics grade trypsin/rLysC (V5071; Promega, Madison, WI, USA) overnight at 37°C according to the SP3 method (Hughes et al ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...