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Citations for Promega :
2001 - 2050 of 2944 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... according to manufacturer’s instructions with the addition of 1 µl RNAse inhibitor (RNAsin Plus RNase Inhibitor, Promega).
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bioRxiv - Systems Biology 2019Quote: ... Proteins were digested on-filter (16 hr, 37 °C) with sequencing-grade modified trypsin (50:1, Promega). Proteolysis was terminated and peptides eluted with 20 μL of 10% trifluoroacetic acid followed by centrifugation (13,000 ×g ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in 400μL FACS buffer (1.5% BSA, 2.5mM HEPES, 1mM EDTA in PBS) containing 2.5% RNAsin and 1/10000 DAPI (Promega). Cells for re-injection experiments were isolated as above and subjected to a further two centrifugation steps (1min at 200xg ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NanoLuc Inhibitor (Promega) diluted 1:500 (10 μL [30 mM stock] per 5 mL Opti-MEM plus substrate) ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Physiology 2020Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Nluc and BRET-FL signals were sequentially acquired by IV-injection with 1/40 diluted furimazine (Promega) in 100 μL sterile PBS and applying open (for Nluc ...
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bioRxiv - Microbiology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
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bioRxiv - Immunology 2019Quote: ... were transfected with indicated 1.33 μg HIV-1 env DNA and 2.66 μg Q23gΔenv using Fugene6 (Promega), harvested 48 hr post-transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed on 1 µg of RNA using ProtoScript First Strand cDNA Synthesis Kit (Promega). Primers recognizing exons flanking the CRISPR-targeted exon (Supp ...
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bioRxiv - Genetics 2019Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of total RNA template was reverse transcribed to cDNA by M-MLV reverse transcriptase (Promega) using specific stem-loop RT primer and under the following conditions ...
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bioRxiv - Genomics 2021Quote: ... Diluted cDNA (1:20) was used in a PCR reaction with the GoTaq qPCR Master Mix (Promega) and run on a CFX384 Touch instrument (Bio-Rad).
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bioRxiv - Developmental Biology 2021Quote: ... DNase I treatment and cDNA synthesis were preformed using the RQ1 RNASE-FREE DNASE 1* (Promega®) and the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific®) ...
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bioRxiv - Biophysics 2020Quote: ... 1 µL of gDNA was added to a standard 24 µL 2X GoTaq Green PCR (Promega, #M7123), typically ∼35 cycles ...
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bioRxiv - Microbiology 2019Quote: ... 1 mL of culture was used for genomic DNA isolation with the Wizard DNA Purification Kit (Promega). Approximately 2 µg of DNA was used to prepare a paired-end 250-bp sequencing library with the Nextera XT DNA library kit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Due to higher incubation temperatures we used 1 μL RNasin Plus Ribonuclease Inhibitor (Promega, Madison, WI, USA). All other methods were identical to previous reverse transcriptions with the exception of changing the main incubation step to 55°C for 1 hour in order to disrupt RNA secondary structure ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was produced from 1 μg of RNA sample using M-MLV reverse transcriptase (Promega; M-1302). Real time qPCR was preformed using a QuantiNova SYBR Green kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... 16 µL of a 1:100 dilution (in 25 mM ammonium bicarbonate) of trypsin solution (Promega V511C) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... for 4h at 37C in 6M urea followed by an overnight trypsin digestion (1:25 ratio; Promega) at 37C in 1M urea ...
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bioRxiv - Genetics 2022Quote: ... Samples were diluted 1:4 with 50mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Biophysics 2022Quote: ... PCR products were separated on 1% agarose gel and purified using a Gel extraction kit (Promega, CA). Sequences of forward and reverse primers are listed in Supplementary Table S21 ...
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bioRxiv - Biochemistry 2022Quote: ... urea was diluted to 1 M by adding 50 mM ammonium bicarbonate and 2 μg Trypsin (Promega) per 100 μg protein ...
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bioRxiv - Cell Biology 2022Quote: ... Trapped proteins were washed four times with methanol TEAB buffer followed by overnight Trypsin (1 μg, Promega) digestion at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: Developing was performed using chemiluminescence reaction (ECL, GE-Healthcare) with secondary coupled to HRP (Promega, 1/5000e) and the Amersham ImageQuant 800 (Cytiva).
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bioRxiv - Systems Biology 2022Quote: ... Proteins were digested by incubation with trypsin (1/100, w/w, Sequencing Grade Modified Trypsin, Porcine; Promega) for at least 9 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... each TECprobe-ML reaction was 48 μl due to the omission of 10X (1 μM) streptavidin (Promega) and 10X Start Solution [100 mM MgCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... pH 3.0) and mixed in a 40:1 molar ratio with commercially purchased pepsin protease (Promega, USA), then incubated at 37°C for 3 h and frozen until later use ...
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bioRxiv - Microbiology 2022Quote: ... viral RNA was quantified by RT-qPCR using the GoTaq Probe 1-Step RT-qPCR System (Promega) on a QuantStudio3 (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Cancer Biology 2024Quote: Protein pellets were dissolved in digestion buffer and digested by 1% ProteaseMAX detergent (Promega, Madison, WI, USA) and Trypsin/Lys-C Mix (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NLuc Inhibitor (Promega) diluted 1:500 (10 µL of 30 mM stock per 5 mL Opti-MEM plus substrate ...
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bioRxiv - Microbiology 2024Quote: ... Then 20 µL of 50 mM ABC containing 1 µg of trypsin (Promega Platinum Mass Spectrometry Grade) was added and the traps were incubated 2h at 47°C in a covered thermomixer without vortexing ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/100, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... ARINA-1 cytotoxicity was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241), which measures intracellular ATP ...
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bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Microbiology 2023Quote: ... After the addition of 1x Nano-Glo Lysis Buffer and 1:1000 Nano-Glo substrate (Promega, USA), relative light units (RLU ...
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bioRxiv - Bioengineering 2023Quote: ... cells were treated with the CellTiter-Glo 2.0 Viability Assay (1:2 v/v; Promega, Madison, WI) using the Viaflo Assist Pipetting Platform (Integra Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Gel bands were washed in 100 mM AmBic/ACN prior to adding 1 µg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NLuc Inhibitor (Promega) diluted 1:500 (10 μL of 30 mM stock per 5 mL Opti-MEM plus substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...
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bioRxiv - Cancer Biology 2023Quote: ... ZR-75-1 cells were short tandem repeat tested every 4 months (Stem Elite ID System, Promega). Doxorubicin (S1208) ...
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bioRxiv - Neuroscience 2023Quote: ... Gel bands were washed in 100 mM AmB/ACN prior to adding 1 µg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µg of total RNA was used to synthesize cDNA using M-MLV reverse transcriptase kit (Promega) using the supplier’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The Nluc and BRET signals were measured (integration time: 1 s) after auto-injecting either Fz (Promega) or FFz (Promega ...