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Citations for Promega :
2001 - 2050 of 5339 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...
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bioRxiv - Genomics 2022Quote: ... PCR fragments were either sequenced directly or cloned using the pGEM-T Easy Vector System (Promega, Cat#: A1360) and Sanger sequenced to resolve insertion characteristics ...
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bioRxiv - Biochemistry 2019Quote: ... The N-terminal 312 aa Halo tag sequence was PCR amplified from His6HaloTag® T7 Vector pH6HTN (Promega) as a 5’ SpeI ...
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bioRxiv - Genetics 2019Quote: ... 0.8 µM of both the FAM labelled forward primer and TPP_M13R and 0.2 µM 5R_TPP using GoTaq® Long PCR Polymerase (Promega). A standard 60TD55 protocol was utilized (94°C denaturation for 30 s ...
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bioRxiv - Genetics 2019Quote: ... and the linearized vector was purified by Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The pure linearized vector and the elpc-2 promoter were fused using an In-Fusion HD Cloning Kit (Takara ...
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bioRxiv - Genomics 2019Quote: ... PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega, Madison, WI, cat# M3005), 10 mM dNTPs ...
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bioRxiv - Plant Biology 2019Quote: ... Amplicons of correct size were gel purified with Wizard® SV Gel and PCR Clean-Up System (Promega). Amplicons were cloned into pDONR/Zeo (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... A PCR reaction was performed with 0.5 U of GoTaq DNA Polymerase in GoTaq Buffer (both from Promega) supplemented with primers and dNTPs to a final volume of 20 µL ...
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bioRxiv - Microbiology 2020Quote: ... A DENV-1 3’UTR specific probe was generated by PCR reaction with GoTaq Polymerase (Promega, Wisconsin, USA) containing DIG DNA-labelling mix (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... This yielded a PCR product of 243 base pairs which is digested with restriction enzyme Bcl1 (Promega, R6651) in the presence of the G>A point mutation (i.e ...
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bioRxiv - Genomics 2020Quote: ... Obtained products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA), and subsequently cloned using the pGEM-T Easy Vector System (Promega ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed in 15 µl reactions using 2x GoTaq DNA Polymerase master mix (Promega Cat. No. M3008) with 5 ng of gDNA and 500 nM of each primer ...
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bioRxiv - Genomics 2019Quote: ... We then set up quantitative PCR reactions with 5 µL GoTaq qPCR Master Mix (Promega, Madison, WI, USA), 1 µL each forward and reverse primer at 5 µM ...
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bioRxiv - Bioengineering 2020Quote: ... PCR reactions were carried out with 100 ng genomic DNA or cDNA in the GoTaq Master Mix (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... The three fragments were cleaned up using the Wizard® SV Gel and PCR Clean-Up System (Promega) and subsequently fused by overlapping PCR ...
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bioRxiv - Biochemistry 2020Quote: ... were transcribed and translated using the TNT T7 Quick for PCR DNA Coupled Transcription/Translation System (Promega, USA). The reactions contained 10 µL of TNT ...
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bioRxiv - Pathology 2021Quote: ... One µL of PCR product was employed to produce the transcripts with the T7 RNA polymerase (Promega, USA), setting a 20 µL-reaction for 2 hours at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... The 10 µL Zn-finger PCR reaction was prepared containing of 2 µL of 5X reaction buffer (Promega), 0.8 µL of 2.0 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... Positive PCR products were purified with the ReliaPrep™ DNA Clean-Up and Concentration System (Promega, Madison, WI). Genomic libraries were constructed with the Nextera XT DNA Sample Preparation kit (Illumina Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... Three μL of purified A-tailed PCR product was then ligated into pGemT®-T vector (Promega Corporation) following guidelines recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... IL-12 and TNFα were analyzed by semiquantitative PCR (SYBR Green assay Go Taq qPCR Master Mix; Promega) with specific primers (Metabion ...
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bioRxiv - Genomics 2020Quote: ... PCR oligonucleotides contained additional sequences complementary to Bgl II restriction endonuclease digested pGL3-promoter plasmid (Promega, Southampton, UK) for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products from the unique regions of her9 were cloned into the pGEMT-easy vector (Promega, Madison, WI). Plasmids were linearized using either NcoI and SpeI restriction enzymes (NEB ...
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bioRxiv - Molecular Biology 2021Quote: 1 uL of pUG cDNA was then input into a 20 uL GoTaq PCR reaction (Promega; Cat # M7123) with the first adapter specific primer (Shukla et al. ...
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bioRxiv - Genetics 2020Quote: ... pGEM vector containing the desired PCR product was transformed into competent JM109 E.coli cells (Promega, Madison, WI, USA). Colonies were evaluated using blue-white screening and PCR with M13 universal primers ...
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bioRxiv - Microbiology 2022Quote: ... The reaction products served as templates for PCR with GoTaq ® G2 Hot Start Master Mix (Promega, USA), 300 nM forward primer ...
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bioRxiv - Genomics 2022Quote: ... The PCR products that showed an obvious difference in HRM were cloned into pGEM-T easy vector (Promega) and sequenced by the Sanger method at the DNA Sequencing and Genotyping Facility at the University of Chicago Comprehensive Cancer Center.
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bioRxiv - Microbiology 2022Quote: ... The GFP and mEOS2 tags were amplified by PCR from peGFP and pmEOS2 using Pfu Polymerase (M7741, Promega), dNTP mix (10297018 ...
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bioRxiv - Molecular Biology 2022Quote: ... Digested plasmids and gBlock DNA fragments were purified by Wizard SV Gel and PCR clean-up system (Promega) and transformed in Stbl3 competent cells ...
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bioRxiv - Microbiology 2022Quote: ... PCR products of attS were further cloned using the pGEM-T easy vector system (Promega, Madison, WI, USA) and DH5α competent cells to analyze the sequence variation of PCR products.
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bioRxiv - Microbiology 2023Quote: ... The fluorescently labeled PCR products were digested by using the 4-bp cutter TaqI (Promega, Southampton, United Kingdom) as described by the manufacturers ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected by using SYBR Green I (GO-Taq PCR Master mix, Promega, Walldorf, Germany; A6002). Suitable reference genes were identified by using geNorm [47] ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected using SYBR Green I (GO-Taq PCR Master mix, Cat: A6002; Promega, Walldorf, Germany). Sequences of the primers used in this study are listed in Supplementary Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was subsequently confirmed using sanger sequencing and transcribed in vitro using T7 RNA polymerase (Promega) and Digoxygenin (DIG ...
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bioRxiv - Cell Biology 2022Quote: ... The A-tailed PCR products were then cloned into the pGEM®-T Easy Vector Systems (Promega #A1360) followed by transformation ...
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bioRxiv - Cancer Biology 2023Quote: ... Conserved Elements (CE) were PCR-amplified from GB genomic DNA using GoTaq® G2 DNA Polymerase (Promega, #M7845) (primer sequences listed in Supplementary Table S1 ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...
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bioRxiv - Immunology 2023Quote: ... the Renilla luciferase (Rluc) gene was PCR-amplified from phRL-TK (Promega, Madison, WI, USA, cat. no. E6241), and the eGFP gene was PCR-amplified from a codon-optimized synthetic gene fragment obtained from Bionics Inc ...
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bioRxiv - Microbiology 2023Quote: ... A PCR product matching the correct target size was gel purified and ligated into pGEM-T Easy (Promega) to yield plcl::Kn ...
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bioRxiv - Genetics 2023Quote: ... PCR products were cleaned with ExonucleaseI (ExoI)(Fermentas; Pittsburgh, PA) and Shrimp Alkaline Phosphatase (SAP) (Promega; Madison, WI) incubated at 37°C for 30 minutes and 80°C for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... Each mutation locus was amplified in separate PCR reactions using GoTaq Green Master Mix (Promega Cat. No. M7123). PCR reactions were held at 95 °C for two minutes followed by 35 cycles of 45 seconds at 95°C for denaturation ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega Corporation, Madison, WI, USA), without fragment separation ...
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bioRxiv - Plant Biology 2024Quote: Each 20 µl endpoint PCR reaction contained 10 µl of Gotaq Green Master Mix (2X) (Promega, Madison, WI), 1 µl (5 µM ...
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bioRxiv - Microbiology 2024Quote: Correct integration of the homology template at the desired location was assessed by PCR (GoTaq DNA Polymerase, Promega) on extracted genomic DNA (Promega Wizard DNA Extraction ...
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bioRxiv - Molecular Biology 2024Quote: ... each WT ZNF410 peak was amplified by PCR from genomic DNA and cloned into pGL4.24 vector (Promega, #E8421), digested by KpnI and XhoI ...
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bioRxiv - Developmental Biology 2023Quote: ... The amplicon was purified prior to sequencing using a Wizard SV Gel and PCR Clean-Up System (Promega).
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted using a DNA extraction kit (Wizard Genomic DNA Purification Kit; Promega Corp, Madison, WI). Genomic DNA was extracted from 1-week old bacterial sub cultures by collecting a loop of bacteria with a sterile pipette tip and inoculating a 0.2 ml PCR tube containing 15 μl of Molecular Grade Water and ran in a thermal cycler for 15 minutes at 95 °C.
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bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
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bioRxiv - Microbiology 2020Quote: ... or GoScript™ Reverse Transcription kit (Promega) and stored at −20°C until analysis ...
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bioRxiv - Microbiology 2022Quote: ... The Dual-Luciferase Assay Reporter Kit (Promega) was used to measure both Renilla and firefly luciferase activity according to the manufacturer’s instructions with the modification that 25 μL of reagent were used with 10 μL of sample ...