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Citations for Promega :
1951 - 2000 of 2480 citations for ssc mir 487b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... Those containing single bright bands were purified using Wizard SV Gel and PCR Clean-up Kit (Promega), and sequenced in both directions using PCR primers at Sequetech Inc ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the dCK gene was PCR-amplified from pDNR-Dual_dCK (DNAsu HsCD00000962) using Pfu DNA polymerase (Promega, Germany) and gene-specific primers (Eurofins Genomics ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 700 ng used for in vitro transcription/translation using TnT T7 Quick for PCR DNA (Promega) and the Fluorotect GreenLys tRNA (Promega ...
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bioRxiv - Molecular Biology 2019Quote: The entire genome was amplified by PCR using GoTaq 2× Hot Start Colorless Master Mix (Promega, USA) using overlapping primer sets (Ali et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed in 10 μL reactions comprising of 1X GoTaq® qPCR master mix (Promega), 1 μM of gene specific forward and reverse primers (sequences are provided in Supplementary Table 3 ...
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bioRxiv - Genomics 2021Quote: ... Diluted cDNA (1:20) was used in a PCR reaction with the GoTaq qPCR Master Mix (Promega) and run on a CFX384 Touch instrument (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... purified from agarose gels using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA) and sent for sequencing (to GATC Services-Eurofin Genomics ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with the BamHI and Sall restriction enzymes and inserted into pGEM4Z (Promega) that had been digested with the same enzymes ...
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bioRxiv - Biophysics 2020Quote: ... 1 µL of gDNA was added to a standard 24 µL 2X GoTaq Green PCR (Promega, #M7123), typically ∼35 cycles ...
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bioRxiv - Biophysics 2021Quote: ... All DNA handles were made by polymerase chain reaction (PCR) using λ DNA cl857 Sam7 (Promega, D1501) as a template and a forward primer containing two digoxigenin labels at the 5’ end ...
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bioRxiv - Microbiology 2021Quote: ... Spike+ cells were then single cell sorted into 96-well PCR plates containing RNase inhibitor (N2611; Promega) and stored at −80 °C.
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bioRxiv - Microbiology 2020Quote: ... The concentration of PCR products was then measured using the QuantiFluor™ dsDNA System (Promega, Madison, WI) and used to pool equimolar amounts of PCR products together ...
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bioRxiv - Immunology 2021Quote: Targeted re-sequencing by the Sanger method (Microsynth AG) was performed on kit purified PCR products (Promega) from chromosomal DNA or expression vector templates using pre-mixed sequencing primers listed Table S2.
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bioRxiv - Immunology 2021Quote: ... isotypes from patients were pooled at equal concentrations and concentrated using Wizard PCR Clean-up kits (Promega) according to manufacturer’s instructions with 30 μl of elute ...
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bioRxiv - Cell Biology 2021Quote: The qRT-PCR reaction was carried out with GoTaq®qPCR Master Mix (Promega, Madison, WI, USA) on a CFX96 instrument (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... DNA was resuspended in 30 uL PCR grade water and quantified by Quantus™ Fluorometer (Promega Inc). The PCR product pools were sequenced by short read sequencing as described by 2.3.1.
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bioRxiv - Developmental Biology 2022Quote: ... The amplified PCR fragments were subcloned into the pGEM T Vector system (Promega Corporation, Madison, WI, USA) and sequenced to confirm each allele.
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bioRxiv - Cell Biology 2022Quote: ... P2-2: -212- -826) were cloned through PCR into the pGL4.20 luciferase reporter vector (Promega, Madison, WI) using C57BL/6 genomic DNA as a template ...
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bioRxiv - Biophysics 2022Quote: ... PCR products were separated on 1% agarose gel and purified using a Gel extraction kit (Promega, CA). Sequences of forward and reverse primers are listed in Supplementary Table S21 ...
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bioRxiv - Bioengineering 2022Quote: ... the EGFP coding sequence was PCR amplified from the plasmid pDestattB_ubi:EGFP using GoTaq® DNA Polymerase (Promega) with attB-P2A-EGFP-F and pA-r primers (Supplementary Table S3) ...
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bioRxiv - Bioengineering 2022Quote: ... The resulting double-stranded DNA fragments were purified using a PCR Clean-up Kit (Promega, Cat# A9282). The purified DNA was then cloned into the entry vectors in place of the respective SERF using Golden Gate techniques [14 ...
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bioRxiv - Cell Biology 2022Quote: ... and aliquots of the cells were analyzed by genomic PCR using GoTaq DNA polymerase (Promega, WI, USA) and specific primers (F_C2 ...
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bioRxiv - Physiology 2022Quote: ... the PCR products were subjected to T/A cloning (pGEM®-T Vector Systems; Promega, Fitchburg, USA) with subsequent Sanger sequencing (Sanger et al ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing rsbU or rsbT/rsbUY28I were subjected to PCR mutagenesis using GoTaq DNA polymerase mix (Promega) supplemented with 2mM additional MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplified fragments were run on an agarose gel and purified using the Wizard SV kit (Promega). The sequences were cloned into the pUASz1.0 vector (54 ...
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bioRxiv - Microbiology 2023Quote: ... as well as 1kB DNA ladder were purchased from New England Biolabs while GoTaq PCR reagents (Promega) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The digested product was gel-extracted using the Wizard SV Gel and PCR Clean-Up Kit (Promega). The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed on a CFX96 q–PCR System using SYBR Green qPCR master mix (Promega, USA). GAPDH was used as the internal control ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were subsequently cloned into vectors using pGEM-T Easy Vector System II (Promega, Cat# A1380) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
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bioRxiv - Microbiology 2023Quote: ... The 3809-bp PCR product was purified and cloned into pGEM-T-Easy (Promega, Medison WI, USA) to yield pGEM ...
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bioRxiv - Developmental Biology 2023Quote: ... A-tailed PCR products were ligated with digested linear pGEM®-T Vector (Promega, Cat. No. A3600), followed by transformation with One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Genomics 2023Quote: ... All PCR analyses were performed on 0.5 ng cDNA using 0.5 U GoTaq® DNA polymerase (Promega). Quantification of PCR products was performed using the Image Lab software (BioRad ...
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bioRxiv - Genetics 2023Quote: ... PCR was performed in a 30 µL reaction volume containing 1X Colorless GoTaq Reaction Buffer (Promega, #M792B), 200 µM dNTPs (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the eluted DNA was subjected to real-time PCR analysis with Eastep qPCR Master Mix (Promega) and promoter-specific primer mixtures ...
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bioRxiv - Genomics 2023Quote: ... Agar was removed from the DNA using the Wizard SV Gel and PCR Clean-Up System (Promega). Transposon-directed insertion site sequencing (TraDIS ...
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bioRxiv - Genetics 2022Quote: ... The digested plasmid was purified using the Wizard SV Gel and PCR Clean-Up System (Promega A9281). The zebrafish exorh promoter region was amplified from pCK029 (this manuscript ...
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bioRxiv - Genetics 2022Quote: Quantitative PCR was performed on the Roche LightCycler 96 instrument by using GoTaq qPCR Master Mix (Promega) with 1µl cDNA template and 0.5µl of 10mM each primer in a total volume of 25µl reaction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The amplified products were gel-purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and then cloned into the pGEM-T Easy Vector (Promega) ...
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bioRxiv - Genomics 2023Quote: ... PCR validation reactions amplification were performed using GoTaq® DNA polymerase (Promega, 1.2 units/50 μl reaction), Colorless GoTaq® Reaction Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... and used 1 μL of cDNA for 10 μL of PCR using 2x GoTaq master mix (Promega) and 250 nM of forward and reverse primer sets with conditions recommended by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using SP6 RNA polymerase and the DIG-labelling kit (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were further digested by TAQI (GoTaq® G2 Flexi DNA Polymerase; Promega, cat. no M7805) to assess if the point mutation is or not present ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCRs were performed under standard conditions for High Performance GoTaq® G2 Flexi DNA Polymerase (Promega; M7801), with the addition of 5x combined enhancer solution [35] per reaction ...
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bioRxiv - Genomics 2024Quote: ... The master mixtures consisted of 1 x Taq&Go G2 Hot Start colorless PCR Master Mix (Promega), 0.5 μM of each primer ...
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bioRxiv - Immunology 2024Quote: ... Quantitative real-time PCR assays were carried out by using GoTaq® qPCR master mix (Promega, Germany) and QuantStudio™ 5 (Thermo fisher scientific ...