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Citations for Promega :
151 - 200 of 2805 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... A3B KO-derivative knockout cell lines were generated by transfecting cells with the pSpCas9(BB)-2A-Puro (PX459) plasmid containing gRNAs targeting A3B with FuGENE 6 Transfection Reagent (E2691; Promega). 24h after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Phoenix-AMPHO packaging cells were transfected with retroviral pBABE-puro-gateway constructs encoding ERBB4 variants or enhanced green fluorescent protein (eGFP) (vector control) using FuGENE 6 transfection reagent (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with pSpCas9(BB)-2A-Puro plasmid containing the sgRNA with Fugene HD transfection reagent (Promega Cat. No. E2311) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were then transiently transfected with the respective pX330-sgRNA-puro constructs using the FuGENE HD Transfection Reagent (Promega, E2311). Transfected cells were then selected with 1 µg/mL puromycin for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... was generated by PCR-cloning the amplification of HaloTag coding sequence (primers HaloTag-For and HaloTag-Rev detailed below (IDTDNA, Coralville, IA)) from pFN21K HaloTag® CMV Flexi® Vector (Promega, Madison, WI), followed by ligation in place of the mApple sequence in the mApple-CD36-C-10 vector (plasmid # 54874 ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were co-transfected with 1 μg of the experimental firefly luciferase construct and 0.2 μg internal pRL control plasmid expressing Renilla luciferase driven by the CMV promoter (Promega, Madison, WI, USA; Cat# E2261). 24 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: Plasmids containing a promoter with binding sites for specific transcription factors to be measured driving firefly luciferase expression were co-transfected with a renilla luciferase reporter plasmid with a CMV promoter (Promega Corp, Madison, WI, USA) using TransIT reagent (0.25 µl/well ...
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bioRxiv - Biophysics 2023Quote: ... was generated by PCR-cloning the HaloTag coding sequence (primers details in Table S1, Integrated DNA Technologies) from pFN21K HaloTag® CMV Flexi® Vector (Promega, Madison, WI), followed by ligation in place of the mApple sequence in the mApple-CD36-C-10 vector (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... containing the -123C wildtype allele, was synthesized at Genscript (Piscataway, NJ, USA) and cloned into the pGL4.20[luc2/Puro] reporter vector (Promega, Madison, WI, USA). A mutant version of this plasmid ...
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bioRxiv - Genomics 2021Quote: ... 1 U μl−1 RNasein (Promega), 0.1% IGEPAL CA-630 (Sigma)) ...
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bioRxiv - Genetics 2024Quote: ... Samples were diluted 1:1 with water and again 1:1 with 2X GoTaq Master Mix (Promega M712B) containing appropriate primers and amplified in 23 PCR cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a secondary antibody (Gpx4 1:10000 or 4HNE 1:10000, W4011, Promega and Gapdh 1:10000 or ActB 1:5000, W4021, Promega), diluted in 10% TBS blocking buffer (Licor ...
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bioRxiv - Immunology 2021Quote: ... A 1:1 dilution of Steady Glo (Promega) and Lysis Buffer were added to the cells and incubated at RT for 15 mins ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... 1 mM CaCl2 with 1 µg trypsin (Promega). Digested proteins were acidified with TFA to pH < 2 and RapiGest SF was precipitated out ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-β-Galactosidase (1:1.000; Promega, 1:1000; abcam), anti-Cleaved Caspase-3 (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µl random primers (50 ng·µl−1, Promega, C1181), 2 µL 0.1M DTT ...
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bioRxiv - Biochemistry 2024Quote: ... 7C (mouse, Promega cat# N700A; 1:500-1:1000); Nluc in Fig ...
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bioRxiv - Cell Biology 2021Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5000, 1:10000 or 1:20000; Promega) was used as secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... and mixed 1:1 v:v with BacTiter-Glo Reagent (Promega). The reaction was incubated for 5 min at room temperature on an orbital shaker ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM dNTP) and 1 μl of AMV RT (Promega) was added and the sample was incubated at 42 °C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and a 1:1 mixture of CellTiter-Glo reagent (Promega) and PBS was added to each well ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in 1:1 PBS:CellTiter-Glo 2.0 (Promega) reagent ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:1 with nuclease-free water (Promega). Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% Digitonin (Promega) and EDTA-free Protease Inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... 1) Trypsin (Promega) and Lys-c (Wako) ...
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bioRxiv - Developmental Biology 2020Quote: ... 1/200 (Promega). The epidermis of WPP was dissected following fillet preparation protocols described in 32.
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bioRxiv - Developmental Biology 2020Quote: ... (1:2000, Promega). Secondary antibodies (diluted 1:200 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:2,000 (Promega); anti-FtsZ ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% Digitonin (Promega) and 50x EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2020Quote: ... duplicate wells were transfected with 1 µg HDM_Spike_RBD_B7-1 plasmids and 1 µg of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Molecular Biology 2023Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5,000; 1:10,000; or 1:20,000; Promega, W4021) was used as the secondary antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were treated with 1 μg ml-1 Proteinase K (Promega) between 1 and 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM CaCl2 and 1 ug of sequence-grade trypsin (Promega) were added and incubation continued overnight ...
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bioRxiv - Neuroscience 2020Quote: ... a caspase-1 selective inhibitor Ac-YVAD-CHO (1 μM; Promega) was added in parallel experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... freshly add 3 µL 1:1 water diluted digitonin (Promega G9441)) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM dNTP) and 1 μl of AMV reverse transcriptase (Promega) was then added and extension was carried out for 1 h at 42 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1% RNAse-free BSA (Gemini) and 1:400 RNasin Plus (Promega) before storage at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... 8μl of Trypsin/LysC solution (100ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 75 µL (1:1 volume) of One-Glo reagent (E6110, Promega) was added to each well and incubated for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 8μl of Trypsin/LysC solution (100ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 250 µL (1:1 volume) of One-Glo reagent (E6110, Promega) was added to each well and incubated for 10 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently Trypsin/LysC solution [100 ng/μL 1:1 Trypsin (Promega):LysC (FujiFilm ...
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bioRxiv - Cancer Biology 2023Quote: ... each well was treated with 1:1 CellTiter-Glo (Promega, UK) and incubated for 10mins at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and anti-HiBiT (Promega #N72000; 1 mg/mL; diluted 1:1000). An HRP-conjugated IgG anti-mouse or anti-rabbit IgG (Proteintech SA00001-1 or SA00001-2 ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA, 5% glycerol) and freshly supplemented reagents (1 mM DTT, 1 mM ATP, 1 mM PMSF, protease inhibitor mix (Promega) and 1% Tween 20) ...
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bioRxiv - Bioengineering 2022Quote: ... To determine the optimum transfection ratio multiple complexes were created (1.5:1, 3:1, 6:1) using ViaFect Transfection reagent (Promega, E4981) and pGL4.51 [luc2/CMV/Neo] plasmid vector (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma], 20 U ml-1 RNase inhibitor [Promega] ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were transfected with 1 μg of pX330 plasmid and 1 μg of the HDR donor plasmid using 1 ug FuGene 6 (Promega). Approximately 2-3 days post-transfection ...