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Citations for Promega :
101 - 150 of 2805 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The K560 sequence was inserted into the pHTC HaloTag CMV neo vector (Promega, Madison, WI, US, G7711) to build a K560-Halo plasmid ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Approximately 1.0 - 1.5 x105 primary hepatocytes were co-transfected per well in 24-well plates with 1.7 μg of each Tc1-Mariner reporter construct together with 0.3 μg of pGL4.75[hRluc/CMV] (Promega) which encodes Renilla luciferase whose activity is used as an internal standard for normalizing variations in cell number and transfection efficiency ...
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bioRxiv - Molecular Biology 2024Quote: ... full-length Nwd1 cDNA was subcloned into the pFN21A-HaloTag-CMV Flexi-vector (Promega, Madison, WI, USA). To construct 3×Flag-SERCA2 (hereafter called Flag-SERCA2) ...
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bioRxiv - Cancer Biology 2024Quote: ... RS4;11 cells were transduced to express the luminescent reporter luciferase using pGL4.51[luc2/CMV/Neo] vector (Promega). Cell authentication was performed using short tandem repeat analysis (Idexx BioAnalytics ...
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bioRxiv - Cancer Biology 2021Quote: 300 × 105 HEK 293T cells were split onto 6 well plates and the next day were mock transfected or transfected with 1.5 ug pTwist-CMV-SLC46A2-exFLAG using 4.5 uL FuGene6 transfection reagent (Promega). 24 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... Halo-V5-METTL14 and Halo-V5-WTAP were cloned into pHTN HaloTag CMV-neo vector (Cat# G7721, Promega) using Gibson assembly methods as previously described (7 ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were co-transfected with TDP-43 expression constructs and the pGL4.50[luc2/CMV/Hygro] luciferase reporter (Promega). After 4 DIV ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
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bioRxiv - Developmental Biology 2024Quote: ... in which Firefly luciferase was replaced with NanoLuc luciferase amplified from the pNLF-N [CMV/Hygro] vector (Promega) by PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... transfected with 8 µg of pLib_EF1A_LuciNeo (kindly provided by Ralf Graeser, ProQinase, Freiburg) or pLib_EF1A_nlstdTomato-2A-puro using FugeneHD (Promega). Medium was exchanged after 4-8 h ...
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bioRxiv - Neuroscience 2024Quote: Sequences of the ChIP peaks were cloned into pGL4.20-Firefly luciferase plasmid (pGL4.20[luc2/puro]; Promega) using HindIII restriction site to generate pGL4.20-ChIPPeak-luc ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-Nluc was generated by replacing TdTomato with Nluc from pNLF1-C [CMV/Hygro] (Promega #N1361) using Gibson Assembly ...
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bioRxiv - Cancer Biology 2021Quote: ... reversely transfected into LNCaP or VCaP (treated with DHT or ETH) cells with a Renilla Luciferase control plasmid pGL4.75 [hRluc/CMV] (Promega) by using X-treme GENE HP DNA Transfection Reagent (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
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bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase reporters were co-transfected together with the pGL4.75 [hRlu/CMV] renilla luciferase reporter (Promega) into either superficial zone chondrocytes ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 320 ng 12x MS2-tagged repeat expression construct and 80 ng CMV-BFP plasmid DNA (for labeling transfected cells) mixed with 1.6 μl FuGENE HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Other plasmids used in this study include the pEGFP-N1 (Clonetech) and pRL-CMV vector (E226A) acquired from Promega. Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: HFF or HEK293 cells (3 × 104 cells per well of a 24-well plate or 1 × 105 cells per well of a 6-well plate) were reverse transfected with 0.5 μg of pCW57-CMV-KDEL-mCherry-GF using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The coding sequence of NanoLuc was amplified by PCR using the primers 5-NanoL and 3-NanoL from pNL1.1.CMV plasmid (Promega). The resulting fragment was digested with BamHI and NotI enzymes and cloned into pEGFP-N1 digested with the same enzymes ...
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bioRxiv - Genomics 2024Quote: ... The cells were co-transfected with pGL3 vector containing the candidate or random EGFP sequence and pGL4.75[hRluc/CMV] vector (Promega) in 1000:1 pmol ratio using Lipofectamine™ 3000 Transfection Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Cell fusion activity was quantitatively determined after 24 h by measuring firefly luciferase activity expressed by pT7EMCVLuc and was standardized with Rluc activity expressed by pRL-CMV by using the Dual-Glo luciferase assay (Promega) (27 ...
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bioRxiv - Molecular Biology 2021Quote: ... NM_001016) and SLC25A6 (accession number: NM_001636) were custom synthesized (IDT) and cloned into pRL-CMV Renilla luciferase vector (Promega, E2261) using oligos in Supplementary file 1 ...
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bioRxiv - Cell Biology 2021Quote: ... KIF3A-HaloTag and KIF9-v2-HaloTag were generated by sub-cloning KIF3A and KIF9-v2 into pHTN HaloTag CMV-neo (Promega). KIF13A ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were then co-transfected with the corresponding pHR plasmid and lentiviral packaging plasmids (pMD and CMV) using Fugene HD (Promega). Cells were incubated for ~ 48 hours and virus was collected and filtered through a 0.45 mm filter ...
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bioRxiv - Bioengineering 2020Quote: VSV-G-pseudotyped lentiviral vectors were produced by co-transfecting HEK293T cells with lentiviral backbone plasmids and packaging plasmids (pHDM-Hgpm2, pHDM-tat1b, pRC/CMV-rev1b, pHDM-G) using Fugene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... while the sequences for NSD2 or NSD3 PWWP1 domain was cloned in-frame into a pNLF1-C [CMV/Hygro] vector from Promega using the In-Fusion HD Cloning kit (Takara) ...
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bioRxiv - Neuroscience 2021Quote: ... Luciferase constructs were obtained by PCR amplifying the 3’UTR regions from cDNA extracts and cloning them into a modified phRL-CMV vector (Promega), as previously described (Favereaux et al. ...
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bioRxiv - Immunology 2022Quote: 1×105 721.221 or 293T cells were plated in 24 well plates and transfected with 400 ng of pGL3 and 100 ng of pRL-CMV (Promega). Two days later ...
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bioRxiv - Molecular Biology 2022Quote: ... expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected six hours later using a Synergy 4 instrument (BioTek) ...
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bioRxiv - Biophysics 2024Quote: ... was incorporated at the 5’ end of the FL open reading frame during PCR amplification from a pRL-CMV vector (Promega). A Kozak consensus sequence and a 50-nucleotide upstream region was incorporated before the translation start site to ensure enough space for the assembly of translation initiation complex77 ...
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bioRxiv - Cell Biology 2023Quote: ... was obtained through transfecting HEK293T cells with these plasmids in combination with a second generation CMV lentiviral packaging system and FuGENE transfection agent (E2311, Promega).
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Biophysics 2023Quote: Wild type TDG-HaloTag and TDG-HaloTag variants were expressed from mutant plasmids constructed utilizing Gene Universal Inc with the pHTN HaloTag CMV-neo vector (Promega).
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bioRxiv - Microbiology 2023Quote: The N protein of SARS-CoV-2 B.1 D614G variant (NYI67-20 strain) was cloned into Nano-Luc vector pNLF1-N [CMV/Hygro] (N terminus) (Cat. N1351, Promega) as a fusion protein ...
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bioRxiv - Cell Biology 2024Quote: ... The HaloTag coding sequence was PCR amplified using KOD FX (KFX-101; Toyobo) from the pFC14A HaloTag CMV Flexi Vector (G965A; Promega). The homology arms for human H3.2 ...
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bioRxiv - Cancer Biology 2024Quote: Plasmids encoding firefly luciferase driven by an estrogen-response promoter element (gift from Dorraya El-Ashry) and CMV-Renilla luciferase (Promega) were co-transfected into cells using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... For these experiments pGL2M4-luc reporter plasmid37 (containing four CACGTG binding sites, a canonical E-box) and pRL Renilla Luciferase Control Reporter Vectors (CMV, Promega E2231) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... A DNA fragment spanning the promoter and the 5′ UTR of Eef2 was PCR-amplified from mouse NIH3T3 genomic DNA and substituted for the CMV promoter of the psiCHECK2 vector (Promega, C802A). The intergenic region between Renilla and firefly luciferase in this plasmid was replaced by the sequence of HCV IRES PCR-amplified from psiCHECK2-HCV-IRES (Iwasaki et al. ...
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bioRxiv - Immunology 2021Quote: Pseudoviruses expressing complete SARS-CoV2 spike genes were prepared by transient transfection of HEK293T cells with three plasmids: SARS-CoV2 MLV-gag/pol and MLV-CMV-luciferase plasmids using Fugene 6 (Promega Inc.) as described earlier (Rogers et al. ...
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bioRxiv - Biochemistry 2023Quote: ... U2OS PINK1 KO cells seeded at a density of 40,000 cells per 18mm glass coverslip in a 12 well plate were transiently transfected with 0.5 ug of plasmid DNA expressing WT-HA PINK1 under the control of a CMV weakened promoter using FuGene HD (Promega, E2311) at a ratio of 1:3 ...
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bioRxiv - Biophysics 2023Quote: N-terminal HaloTag fusion construct for BRG1 (pHTN-BRG1) was generated by digesting the pHTN HaloTag® CMV-neo vector (G7721, Promega) with PvuI and XbaI ...
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bioRxiv - Biophysics 2023Quote: C-terminal HaloTag fusion construct for BAF57 (pBAF57-HTC) was generated by amplifying the HaloTag backbone from the pHTC HaloTag® CMV-neo vector (G7711, Promega) and digesting it with AgeI and KpnI ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells seeded in 12-wells plates coated with poly-D-lysine were transfected with 500 ng of empty vector pHTN HaloTag CMV-neo (Promega G7721) or pCEFL-HaloTag-P-Rex1 constructs and co-transfected with 500 ng of SRE-firefly luciferase and 50 ng of Renilla luciferase plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... Cloning of the mNeonGreen fused shelterin proteins was performed by Gibson assembly into pRK2 backbone (original pHTN HaloTag CMV-neo, Promega #G7721).
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bioRxiv - Microbiology 2024Quote: ... preceding an immunoglobulin gene heavy chain variable region that is commonly found in commercial vectors such as in the pRL-CMV plasmid from Promega (Fig 1A) [53–55].
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bioRxiv - Cell Biology 2024Quote: ... The DLRTM assay is comprised of a Notch Response Element (NRE) Firefly luciferase reporter (p10xRbpj-luc)49 to measure NOTCH3 signalling activity and a constitutively expressed pRL-CMV (Promega, WI, USA) construct as an internal control for cell transfection efficiency and cell viability ...
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bioRxiv - Molecular Biology 2022Quote: ... Retroviral supernatants were obtained from 293GP cells co-transfected with pVSV-G and pBABE-puro-hTERT or pBABE-hygro-KRAS-V12 constructs (kind gifts from Jerry Shay) using FuGENE HD (Promega) and passed through a 0.45 um filter ...
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bioRxiv - Biochemistry 2023Quote: ... by transfection of the pSpCas9(BB)-2A-Puro (PX459) plasmid containing A3B gRNAs with FuGENE 6 Transfection Reagent (E2691; Promega). 16h after transfection ...
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bioRxiv - Biophysics 2023Quote: ... PB-mClover3-H2B::PGK-BSD and PB-mRuby3-NPM1::PGK-Puro were co-transfected with CAG-PBase into HeLa S3 using a FUGENE HD transfection kit (Promega). Stable clones were obtained by sequential Puromycin and blasticidin selection ...
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bioRxiv - Molecular Biology 2024Quote: ... A3B KO-derivative knockout cell lines were generated by transfecting cells with the pSpCas9(BB)-2A-Puro (PX459) plasmid containing gRNAs targeting A3B with FuGENE 6 Transfection Reagent (E2691; Promega). 24h after transfection ...