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Citations for Promega :
151 - 200 of 1074 citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Recombinant bacmid DNA was isolated using the SV genomic DNA isolation kit (Promega) using manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: Ni-NTA beads were firstly digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Biochemistry 2021Quote: ... treated with 3 mM dithiothreitol (DTT) (molecular grade, Cat. 3483-12-3, Promega), and incubated for 30 min at 37°C in 5% CO2 in serum-free RPMI media ...
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bioRxiv - Microbiology 2020Quote: ... 500 ng human genomic DNA (Promega) or 5 μl of untreated or RNase-treated nasal swab nucleic acid isolates ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled human male genomic DNA (Promega) was randomly sheared (500-800bp ...
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bioRxiv - Systems Biology 2021Quote: ... a human cDNA pool (Promega Corporation), or obtained as synthesized double-stranded DNA fragments (gBlocks ...
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bioRxiv - Genomics 2021Quote: ... 50ng human genomic DNA (Promega, G304A), 2µM assembled T7-Tn5 transposase or Tn5 transposase was incubated at 55 °C for 7minutes ...
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... human Female genomic DNA (Promega #G1521), as a reference ...
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were resuspended in reaction buffer [RNase inhibitor (0.2U/μL Recombinant RNAsin (Promega, PAN2515), 1% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
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bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Immunology 2020Quote: ... The following day the cells were transfected with plasmids encoding the indicated FLAG-tagged protein (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h cells were harvested and washed 3 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Ubiquitin enrichment was performed using Halo-tagged MultiDsk TUBE (Wilson et al., 2012) preincubated with HaloLinkTM resin (G1913, Promega) for 1 hour with rotation at RT in binding buffer (100mM Tris (pH 7.5) ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged CXCR2/CXCR3/CXCR7 and 3.5μg of F22 (Promega, Cat. no: E2301). 14-16h post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase-3 and -7 activity was measured by Caspase-Glo 3/7 Assay (Promega) according the manufacturer’s instructions using a BioTek Synergy H1 plate reader.
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bioRxiv - Cancer Biology 2021Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega) and caspase activity was normalized to cell number by performing the CellTiter-Glo Luminescent Cell Viability Assay on the duplicate plate.
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega), and caspase activity was normalized to cell number by performing the CellTiter Glo Luminescent Cell Viability Assay on the duplicate plate ...
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bioRxiv - Cancer Biology 2022Quote: ... caspase-3 and -7 activities were measured by Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2020Quote: ... Caspase-3/7 activities were measured using Caspase-Glo 3/7 Assay kits (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μg of purified RNA was incubated with 3 units of RQ1 DNase (Promega) at 37°C for 30 mins ...
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bioRxiv - Bioengineering 2021Quote: Caspase 3/7 activity was measured using the Caspase-Glo 3/7 assay (Promega). Caspase 3/7 assay solution was mixed 1:1 with αMEM without phenol red (ThermoFisher Scientific ...
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Class IIa HDACs inhibit cell death pathways and protect muscle integrity in response to lipotoxicitybioRxiv - Cell Biology 2023Quote: ... caspase 3 activity was measured using Caspase-Glo 3/7 assay (Promega, Alexandria, Australia). Quantification of viable cells was performed by staining with 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase 3/7 Glo (Promega) was used to measure caspase 3/7 cleavage ...
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bioRxiv - Biochemistry 2022Quote: ... 3 units/mL RNasin (Promega). Depending on the experiments (see results) ...
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bioRxiv - Microbiology 2020Quote: ... caspase-3/7 activity was quantified using the Caspase-Glo-3/7 assay system (Promega). Luminescence was quantified using plate reader Synergy H1 Hybrid Reader (BioTek).
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bioRxiv - Cancer Biology 2021Quote: ... Caspase-3/7 activity was measured using Caspase-Glo 3/7 Assay Systems (G8091, Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... caspase-3/7 activity was quantified using the Caspase-Glo-3/7 assay system (Promega). Luminescence was quantified using plate reader Synergy H1 Hybrid Reader (BioTek).
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bioRxiv - Immunology 2019Quote: ... RAC2 3’UTR-: 5’-TTGCGGCCAGCGGCCGCTCTCCAAACTTGAATCAATAAATTT-3’ and inserted into a dual luciferase psiCHECK2 backbone (Promega). RAC2 mutant 3’UTR constructs were generated using Infusion HD cloning kit (Clontech ...
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bioRxiv - Immunology 2021Quote: Caspase 3/7 activity was measured using Caspase-Glo® 3/7 Assay (Promega, G8090) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase 3/7 activity was assessed using the Caspase-Glo 3/7 assay reagent (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The intracellular caspase-3/7 activity was measured using Caspase-Glo 3/7 Assay (Promega). For this ...
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bioRxiv - Cell Biology 2022Quote: ... Caspase 3/7 activity was measured by adding Caspase-Glo 3/7 Assay Reagent (Promega), incubating for 45 min at room temperature and measuring luminescence in an EnVision 2104 Multilabel plate reader (PerkinElmer).
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bioRxiv - Microbiology 2022Quote: ... for Calu-3 cells and CellTiter-Glo 3-D Cell Viability Assay Kit (Promega, Germany) for hBAECs according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Caspase-3/7 activity was detected using the Caspase-Glo 3/7 Assay Kit (Promega) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 cleavage activity was measured by Caspase-Glo 3/7 Assay Kit (Promega). 1 ×104 cells/well were plated in a pre-coated 96-well plate ...
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bioRxiv - Biochemistry 2019Quote: ... Dimeric constructs are based on VY208 and were created by artificially dimerization through an N-terminal GST-tag (Reck-Peterson et al, 2006) and tagged with a HaloTag (Promega) at the C-terminus as well as a GFP at the very N-terminus ...