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Citations for Promega :
101 - 150 of 1074 citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... by PCR and cloned into the Halo-tagged pFN21A vector (Promega, Madison, WI, USA). The AMPKγ1 mutant vector wherein Ser260 and Thr262 were replaced with alanine was created using parent-template backbone mouse AMPKγ1- full-length vector (# 15996 ...
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bioRxiv - Biochemistry 2023Quote: ... 10 nmol of HALO-tagged UBDs were immobilized on 100 μl HALOLink resin (Promega) in 500 μl HALO-coupling buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and 75 μg of Rho-tagged OR in the pCI mammalian expression vector (Promega) were transfected 18 to 24 h before odorant stimulation using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and 75 μg of rho-tagged OR in the pCI mammalian expression vector (Promega) were transfected 18–24 h before odorant stimulation using Lipofectamine 2000 (11668019 ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: HPV16 integration into the IRF4 gene was confirmed by PCR using GoTaq Long PCR Master Mix kit (Promega U.S.), with 5’-AAGCATGTCAGACACGCAGA- 3’as a forward primer ...
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bioRxiv - Microbiology 2019Quote: ... The 1400 bp long DNA fragments were purified (Wizard®SV Gel and PCR Clean-Up System, Promega, Germany) and sequenced using the same 27f and 1492r primer pair ...
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bioRxiv - Microbiology 2023Quote: ... UTI-59 DNA for long-read sequencing only was extracted using the Wizard HMW DNA Extraction Kit (Promega, USA) following the manufacturer’s instructions excepted eluted in molecular grade water ...
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bioRxiv - Immunology 2020Quote: ... were pre-coated with recombinant luciferase (0.4μg/ml, Promega, #E1701) or spike protein (2μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
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bioRxiv - Bioengineering 2023Quote: ... several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve ...
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bioRxiv - Cell Biology 2022Quote: ... After 3 days Caspase-Glo 3/7 (Promega) and CellTiterGlo (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... The 3xFlag-m-PKD1-2xMyc/His insert was subcloned into pCI vector (Promega). To produce CTF expression constructs of human (h ...
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bioRxiv - Molecular Biology 2020Quote: A Myc-tagged construct was synthesized by Genewiz and cloned into the psiCHECK2 vector (Promega), replacing the synthetic Renilla Luciferase gene ...
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bioRxiv - Biochemistry 2022Quote: ... with or without RFP-tagged full-length Caskin1 using ViaFect Transfection Reagent (Promega, Madison, WI) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Myc-tagged DHX36 was constructed by inserting the ORF of DHX36 into pCMV-Myc (Promega). pHAGE-WT and -D126A DDX21 were constructed by inserting the flag-tagged WT and D126A DDX21 into pHAGE-bsd ...
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bioRxiv - Biochemistry 2022Quote: ... The preparation of Halo-tagged NEMO and its covalent attachment to Halo-Link Resin (Promega) have been described (Emmerich et al. ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-tagged TFs were expressed with TnT® Coupled Wheat Germ Extract System (Promega, USA) using 2 µg plasmid DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... purified Halo-tagged MultiDsk (15 μg per condition) was pre-incubated with HaloLink resin (Promega) for 1 h with rotation at room temperature in binding buffer (100 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we implemented a cell-free assay with purified recombinant luciferase (Promega) and luciferin (BrightGlo Luciferase Assay System ...
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bioRxiv - Bioengineering 2020Quote: ... The standard curve was prepared by diluting recombinant ATP (Promega, P1132). For both assays ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7 and digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant bacmids were transfected into Sf21 cells using Fugene6 (Promega E2693). Supernatant containing viruses (V0 ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease Inhibitor (1 uL/ml, Promega, Cat#N2511). The components of the high salt wash buffer are as follows ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant baculovirus was generated using FuGENE HD Transfection reagent (Promega) in Sf9 cells using Insect X-press (Lonza ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with plasmids encoding fluorescently tagged proteins using FuGENE® HD Transfection Reagent (Promega), following manufacturer’s protocol and assayed 1-3 days after transfection ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged SPUD in vitro expression was done with Transcend Non-Radioactive Translation Detection System (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
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bioRxiv - Immunology 2022Quote: ... 40% glycerol and 0.4 U/μl recombinant RNase inhibitor (Promega, Cat# N2615) and then incubated at room temperature for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 50 ng of plasmids encoding the HiBiT-tagged proteins using FuGENE HD (Promega). Cells were trypsinized (Gibco ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Caspase-Glo 3/7 Reagent (Caspase-Glo® 3/7 Assay, Promega) was added to the wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was detected using Caspase-Glo 3/7 (Promega). Annexin V/Propidium Iodide staining was performed using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... caspase-3/7 levels were examined using Caspase-Glo 3/7 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... or 3) Pepsin (Promega). 1 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL RNasin (Promega) and 100 µL lysis buffer for a total volume of 300 µL for IP by rotation for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and recombinant plasmids were isolated using the Pure Yield Plasmid Miniprep System (Promega). Sizes of purified plasmids were verified by agarose gel electrophoresis ...
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bioRxiv - Physiology 2020Quote: ... using recombinant active SGK1 as a positive control following the manufacturer’s instructions (Promega). The kinase and ATPase reactions of SGK1 were carried out at 30°C for 1 hr using 400 μM ATP and a designed peptide (Murray et al. ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease inhibitor (1 uL/ml; Promega, Madison, WI; Cat#N2511). These washes were followed by an additional wash with 500 μl each of both wash buffer and high salt wash buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease inhibitor (1 ul/ml; Promega, Madison, WI; Cat#N2511) with a mechanical homogenizer followed by addition of TURBO DNase (2 μl ...