Labshake search
Citations for Promega :
151 - 200 of 1023 citations for Polystyrene Latex Beads 5 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2022Quote: ... The washed beads-proteins were then released into the trypsin (Promega, V5111)-containing deepwells at a 50:1 (w/w ...
-
bioRxiv - Microbiology 2022Quote: ... The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega) and verified by Western blotting with the anti-HaloTag antibody (Promega) ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were on-bead digested using 500 ng Sequencing Grade Trypsin (Promega). The digestion was carried out at 37°C overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant without beads was digested with 150ng trypsin (Trypsin Gold, Promega) in 1.5µl 50mM ammonium bicarbonate followed by incubation at 37°C for 5 hours ...
-
bioRxiv - Genomics 2023Quote: ... The cDNA was then purified with ProNex magnetic beads (Promega, WI, USA) according to the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences ...
-
bioRxiv - Biochemistry 2023Quote: The pull-down samples were treated on-beads by trypsin/LysC (Promega). The resulting peptides were loaded and separated on a C18 columns for online liquid chromatography performed with an RSLCnano system (Ultimate 3000 ...
-
bioRxiv - Cell Biology 2023Quote: Lysates were digested from the beads with 100 ng of LysC (Promega) in 25mM HEPES pH 8.5 overnight at 37 °C with shaking at 1200 RPM in a thermomixer ...
-
bioRxiv - Neuroscience 2024Quote: ... Bound antigen-antibody complexes were precipitated using streptavidin-coated magnetic beads (Promega) for at least 1 hr at 4°C ...
-
bioRxiv - Plant Biology 2024Quote: ... Soluble fractions of lysate were added to triplicates of MagneGST beads (Promega) suspended in equal volumes of 0.5M D-mannitol PBS and incubated at 4°C for 1.5hrs undergoing end-over-end mixing to bind GST-tagged transcription factor proteins ...
-
bioRxiv - Neuroscience 2024Quote: ... On-bead digestion was performed with 0.25 µg sequence-grade trypsin (Promega) in 100 mM Tris ...
-
bioRxiv - Genomics 2024Quote: ... the library was purified with ProNex beads (Promega, Madison, WI; Cat. # NG2002) for two PCR amplification conditions at 15 cycles each then another ProNex beads purification ...
-
bioRxiv - Cancer Biology 2024Quote: ... The beads were then incubated with sequencing grade trypsin (Promega, Madison, WI) overnight at room temperature to release tryptic peptides ...
-
bioRxiv - Biochemistry 2021Quote: ... and then cells were pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
-
bioRxiv - Biochemistry 2021Quote: ... and then cells are pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The 5’ UTR of mouse 5’-tRFCys targets or non-target Gapdh were cloned into psiCHECK2 (Promega). Synthetic 5’-tRF-Cys antisense or a scrambled control sequence embedded in the tough decoy backbone (Haraguchi et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... gDNA was amplified using primers 5’-AGTCCCTTCCTTGTCACTTAGT-3’ and 5’-ATCTCACAAGAAAGCGAAATCC-3’ and GoTaq DNA Polymerase (Promega), then digested using EcoRV (New England Biosciences) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The primer pair (5′- accagacggagtttgagcgcgtcttcTGAGGAGGATCCGGTGGAGCTAGCGGAAGA-3′ and 5′-ctccaccgagtcgtactgcttcgccatACCAGAATTCCCACCGCTCGAGCCA-3′) and pBit3.1-N (Cat. No. N2361, Promega) were used to clone the vector-containing fragment ...
-
bioRxiv - Genetics 2024Quote: ... using primers 5’-TCCCTTCCTTCAAGGCTACA-3’ and 5’-GTTAGGAGCCAGAGCAGCAC-3’ and the Go-Taq Flexi DNA Polymerase (Promega), according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 5 µL of cell supernatant was mixed with 5 µL of a nanoglo substrate:buffer solution (Promega, N1110) at a 1:50 ratio in a black 384-well plate and analyzed using a multiplate reader ...
-
bioRxiv - Developmental Biology 2020Quote: Proteins were digested on-bead with 0.5 ug Sequencing Grade Modified Trypsin (Promega) overnight at 37°C with agitation ...
-
bioRxiv - Neuroscience 2021Quote: ... proteins were directly digested on the beads by trypsin (Promega, Madison, WI, USA) overnight at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
-
bioRxiv - Immunology 2022Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described (35 ...
-
bioRxiv - Biochemistry 2020Quote: ... Reacted sequences were isolated by pull-down with Streptavidin MagneSphere paramagnetic beads (Promega) and eluted with formamide/EDTA ...
-
bioRxiv - Plant Biology 2021Quote: ... The protein expression reaction was mixed with HaloTag-ligand conjugated magnetic beads (Promega) to pull down the HaloTag-fused TF ...
-
Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... The proteins enriched by neutravidin beads were digested with MS-grade trypsin (Promega). The on-beads digested peptides were collected and desalted by the in-house prepared reversed phase C18 Stage-tip ...
-
bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with 6.7 μM HaloTag-TMR ligand (Promega) for 15 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... Beads-biotin-probes-RNA adducts were captured by magnets (Promega, Madison, WI, USA) and washed five times with the Wash buffer (2X SSC ...
-
bioRxiv - Immunology 2020Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described (27 ...
-
bioRxiv - Plant Biology 2022Quote: ... beads were flash frozen in liquid nitrogen and trypsinized via standard protocols (Promega). Peptides were labeled with 8-plex iTRAQ66 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The reaction was then mixed with 10 μl Magne HaloTag Beads (Promega, G7282) and 50 μl wash buffer (PBS+0.05% NP40 ...
-
bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
-
bioRxiv - Genomics 2023Quote: ... Long cDNA (> 2kb) fragments were selected with ProNex magnetic beads (Promega, WI, USA), and at least 80 ng were used to prepare the cDNA SMRTbell library ...
-
bioRxiv - Molecular Biology 2023Quote: Ni-NTA beads were firstly digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
-
bioRxiv - Systems Biology 2023Quote: ... Halo-fusion proteins were purified with Magne® HaloTag® Beads (Promega, USA) and then incubated with 50 ng ampDAP library ...
-
bioRxiv - Immunology 2023Quote: ... The supernatant without beads was digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µL 50 mM ammonium bicarbonate followed by incubation at 37°C for 5 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... Eluate obtained above was then incubated with Magne® HaloTag® Beads (Promega) at 4°C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... The eluted fraction thus obtained was next incubated with Magne HaloTag Beads (Promega) at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... and IgG captured and eluted from Magne Protein A beads (Promega, Madison, WI) as previously described 18,19 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The supernatant without beads was incubated with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µl 50 mM ammonium bicarbonate at 37°C for 5 h ...
-
bioRxiv - Plant Biology 2024Quote: ... HALO-TF protein was subsequently incubated with 10 ug of MagneHALO beads (Promega) for 1 hour rotating at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... In-solution on-bead digestion of proteins was performed using Immobilized trypsin (Promega, sample-enzyme solution ratio of 94 (v/v) ...
-
bioRxiv - Microbiology 2024Quote: ... On-bead digest was performed with 0.25µg Trypsin (Gold, Mass Spectrometry Grade, Promega) and 0.25 µg Lys-C (Wako ...
-
bioRxiv - Plant Biology 2024Quote: ... The HALO-fused CBF proteins were purified thanks to Magne HaloTag beads (Promega). A control sample corresponding to HaloTag protein alone was used (referred to as pIX-HALO empty) ...
-
bioRxiv - Plant Biology 2024Quote: ... Halo-fusion proteins were purified with Magne® HaloTag® Beads (Promega, USA) and then incubated with 50 ng ampDAP library ...
-
bioRxiv - Molecular Biology 2024Quote: ... The fusion proteins were directly purified using Magne Halo Tag Beads (Promega, USA). HaloTag–transcription factor fusion proteins were incubated with an adaptor-ligated genomic DNA library ...
-
bioRxiv - Biochemistry 2024Quote: ... the beads were suspended with passive lysis buffer (PLB) (Promega, Madison, WI, USA). Aliquots were taken and luciferase activities were measured using a Nano-Glo® Luciferase assay reagent kit (Promega ...
-
bioRxiv - Cell Biology 2024Quote: ... The beads were resuspended in 100 mM Tris pH 8.0 and trypsin (Promega) was added to each sample in an estimated 50:1 protein:enzyme ratio for overnight digest at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: ... Biotinylated HLA-B*73:01 was immobilized onto streptavidin-coated paramagnetic beads (Promega) for the selection process ...
-
bioRxiv - Cell Biology 2024Quote: ... Beads were then washed in NP40 lysis buffer and DNaseI (20 U, Promega) was added for 15 min at 37°C following addition of 0.1% SDS and 0.5 mg/ml Proteinase K (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were incubated ∼18 hours at 37°C and individual white colonies screened for insert by colony PCR using primers M13_F (5’-ACGACGTTGTAAAACGACGGCCAGT-3’) and M13_R (5’-ATTTCACACAGGAAACAGCTATGACCA-3’) GoTaq DNA Polymerase (Promega). Reactions were separated by gel electrophoresis ...