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Citations for Promega :
451 - 500 of 1023 citations for Polystyrene Latex Beads 5 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and digested on-bead using a filter-aided sample preparation (Wiśniewski et al., 2009) with sequencing grade-modified porcine trypsin (Promega). Tryptic peptides were trapped and eluted on 3.5um CSH C18 resin (Waters ...
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bioRxiv - Biochemistry 2024Quote: ... was added to 0.5 mL 1% bovine serum albumin (BSA) in IP/lysis buffer (Pierce, 87788) and incubated with MagneHis beads (Promega, 32 µL suspension ...
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bioRxiv - Cell Biology 2021Quote: To analyze the 5’ UTR variants we used the pGL3-Basic (Promega), amplification of the 1.4 kb of the 5’ UTR region was carried out using Phusion High Fidelity polymerase (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µl of 5X GoTaq Flexi reaction buffer (Promega, Madison, WI, USA), 2 mM of MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... the concentrated peak fractions were incubated with 5 μM Halo-Alexa488 (Promega) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 5 μM coelenterazine h (Promega, Madison, WI, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of 50 μM stock solution of TMR dye (Promega G8251) was added to the concentrated culture ...
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bioRxiv - Microbiology 2022Quote: ... 1 complete protease inhibitor tablet EDTA-free and 5 µl DNaseI (Promega), lysed using a cell disruptor and the insoluble fraction removed by centrifugation (15,000 xg ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl of 5 ng μl−1 trypsin (sequencing grade, Promega®) in 50 mM AHC plus 1 mM CaCl2 were added to each well and incubated for 20 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1.0 μM 431R2 [5’-CTCTTCACAACAGTCATGTGCG-3’] and 1.0 U GoTaq2 polymerase (Promega). Cycling conditions were 30 s at 98°C ...
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bioRxiv - Cell Biology 2021Quote: ... GST-dimerized dynein constructs were labeled with 5 mM Halo-TMR (Promega) in the column for 10 min at room temperature and the unbound dye was washed away with TEV buffer at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 5 μl/well of CellTiter 96 Aqueous One Solution (Promega, Madison, WI) was added to the bottom wells and the absorbance at 490 nm (A490 ...
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bioRxiv - Molecular Biology 2022Quote: ... One to 5 µg of RNA were digested with DNase (Promega M6101) and reverse-transcribed to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems 4368813) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5 μl of 1.5 mM GoTaqFlexi DNA polymerase Mix (Promega no. M8305), 1 μl of genomic DNA and 2.4 μl of H2O ...
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bioRxiv - Microbiology 2024Quote: ... which included a 5 µl nucleotide mix containing 2.5 mM NTPs (Promega). For each reaction ...
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bioRxiv - Biochemistry 2023Quote: ... and cells were chased with HaLo-Tag TMR ligand (5 µM, Promega) in Opti-MEM for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... before introduction of 2 μl seeds (diluted 1:5) using MultiFectam (Promega), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... PSC proliferation was followed for 5 days with CellTiter-Glo (G7572; Promega) with measurements every 24 hours.
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bioRxiv - Cancer Biology 2023Quote: ... Viability was determined after 5 days using CellTiterGlo reagent (Cat #G9683, Promega) and detected using FluoStar Optima Plate Reader (21999 ...
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bioRxiv - Bioengineering 2023Quote: ... 5 µL reactions were made by adding 4 µL LgBiT (Promega, N1120), 0.5 µL 100 µM p86-R4 (Vivitide ...
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bioRxiv - Cancer Biology 2023Quote: ... The viability was measured after 5 days with CellTiter Glo 2.0 (Promega). For more details ...
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) and Ndc80c was fluorescently labeled with HaloTag TMR ligand (Promega) for 2 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... or 5 µL qPCR plasmid pJEB029 (diluted serially in lambda DNA (Promega)) were added ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μL culture were added to 45 μL NanoGlo reagent (1x Promega lysis buffer and NanoGlo reagent ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 µL 1:25 diluted Nano-Glo luciferase assay substrate (Promega, N113A) in PBS was dispensed into each well and quickly mixed ...
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bioRxiv - Microbiology 2024Quote: ... On-column digestion occurred using 5 ug trypsin (Promega sequence-grade trypsin) in 50 mM TEAB for 3 hours at 47C ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were incubated with 5 µM of Coelenterazine-h (CTZ-h, Promega) for 5 min before 2 pre-read measurements were taken ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed 5 days later using CellTiter-Glo 3D (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Indexed libraries were then cleaned using AMPure XP beads and quantified on the Quantus Fluorometer using the QuantiFluor ONE dsDNA System (Promega). Amplicons were purity-checked and sized on a TapeStation using D1000 ScreenTape System (Agilent) ...
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
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bioRxiv - Cell Biology 2021Quote: ... which were washed with a series of harsh washes before cross-linker reversion and on bead digest with Trypsin Lys-C (Promega). Peptides were then desalted and prepared for mass spectrometry ...
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bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... On-bead digestion was performed by overnight incubation at 37°C with 2 μg of mass spectrometry grade trypsin (Promega). The following morning an additional 2 μg of trypsin was added to each sample and incubated at 37°C for 4-6 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... mix was used at a concentration of 100 μg/μl for overnight “on-bead” digestion using LysC-trypsin mix (Promega)2,3 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Genomics 2022Quote: ... Then the gel beads were washed with DPBS three times and 200 μL of proteinase K solution (1 mg/mL proteinase K [Promega, Madison ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Microbiology 2021Quote: The hydrolytic activity of immunoprecipitated PDEs bound to anti-HA magnetic beads was measured using the PDE-Glo assay (Promega), a bioluminsescence-based assay which quantifies the amount of cAMP or cGMP hydrolysed by a given PDE ...
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bioRxiv - Bioengineering 2020Quote: Phage selections were done according to previously established protocols.30 Selections were performed using biotinylated antigens captured with streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Pathology 2020Quote: The cellular ATP levels were quantified in 100 μL ATP assay buffer or from 20-30 mg wound skin harvested in 200 μL assay buffer and lysed with the bead shocker following the manufacturer’s instructions (CellTiter-Glo luminescent ATP assay kit, Promega, Madison). The protein content from identical treated cells or tissues were determined by BCA Protein Assay kit for normalization (Young ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein complexes were directly digested on-beads by the addition of 2 μg of a Trypsin/Lys-C mixture (Promega). Peptides were isolated on C18 resin tips according to the manufacturer’s instructions (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... enriched proteins were digested at 37 °C overnight on agarose beads in RapiGest SF solution (Waters, 186001861) supplemented with 0.75 μg trypsin (Promega, V511A). The reaction was stopped using 1% TFA (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was removed and tryptic peptides were eluted from the beads by overnight digestion in 50 µl trypsin solution (V5111, Promega, 0.01 µg/µl in 100 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were processed using the SP3 protocol (Hughes et al.2014) and on-bead digested with trypsin (sequencing grade, Promega), which was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... beads were resuspended in 100 μL of 50 mM TEAB and enzymatically digested overnight using sequencing-grade trypsin (Promega, V5111). Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2022Quote: ... 120 μg of fragmented total RNA was added to the antibody-bound beads in 250 μl IP buffer supplemented with RNasin Plus RNase inhibitor (PRN2615, Promega), and the mixture was incubated at 4 °C for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... the washed beads were re-suspended in 150 µl trypsin digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...