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Citations for Promega :
151 - 200 of 234 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Cancer Biology 2022Quote: ... Pull down was performed as described in the Halo tag technical manual TM342 (Promega). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... SEPT6-HALO was made by cloning the HALO tag from pFN21K (Promega cat# G2821) onto the N-terminus of SEPT6 from SEPT6-GFP-pLVX-IRES-puro ...
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bioRxiv - Biochemistry 2019Quote: ... containing no tandem repeats of rare codons was PCR amplified from Eh genomic DNA with the specific primers EhTopoIIfgSBamHI and EhTopoIIfgASXhoI (S1 Table) using High Fidelity Taq polymerase (Thermo Fischer Scientific, USA) and subcloned into pGEMT-Easy (Promega, USA) via TA ligation ...
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bioRxiv - Pathology 2019Quote: ... Then the full DNA-A of TYLCV-C85S was amplified using the primers listed in S1 Table and inserted into the pGEM-T Easy (Promega, USA) vector to produce pGEM-1A-C85S ...
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bioRxiv - Plant Biology 2022Quote: A 1531 bp segment upstream of the transcription start site was isolated from Arabidopsis genomic DNA with specific oligonucleotides (Supplementary Table S1) and cloned into the pGEM®-T Easy (Promega). After that ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug combinations were performed by treating cells with varying concentrations of the drugs/inhibitors (Table S1) and cell viability was measured by CellTiterGlo assay (Promega, G7572).
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the ORF8 plasmid together with the transposase coding plasmid (1/10) with Fugene HD (Promega, Germany) and after one day selected with 3 µg/ml puromycin for three days ...
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bioRxiv - Neuroscience 2021Quote: Luminescence assays to monitor cAMP levels were performed as we described previously (Siuda et al., 2015). Briefly, HEK293 cells (ATCC, cat. # CRL-1573) were co-transfected with PPO-Venus and pGloSensor-22F (Promega E2301) plasmids using JetPrime reagent (Polyplus ...
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bioRxiv - Molecular Biology 2023Quote: ... NLS-NSD2-mVenus WT and T1150A plasmids were transfected into 70 % confluent HEK293 SETD2 knockout cells using FuGENE® HD Transfection Reagent (Promega). NLS-mVenus empty vector was used as a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... a mixture of the 3 Cas9-sgRNA plasmids (150ng/μl) was transfected into HEK293 cells at 70% confluency in fresh medium using FuGENE® HD Transfection Reagent (Promega). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC-aCM or HEK293 cells were incubated with a labeling solution containing both cell-impermeant HTL-Alexa488 (Promega G1001, 1 µM) and Cellmask Deep Red Plasma Membrane stain (Invitrogen C10046 ...
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bioRxiv - Biochemistry 2021Quote: ... the aqueous supernatant was transferred to a new tube to which 250 pg of a luciferase control RNA spike-in (luc, Promega). RNA was precipitated overnight at −20°C by the addition of 1 volume of isopropanol and 2 μL of GlycoBlue (15 mg/mL ...
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bioRxiv - Microbiology 2021Quote: Replication-deficient SARS-CoV-2 Spike pseudotyped lentivirus particles were generated by co-transfecting GFP-luciferase vector and ancestral4 or Beta18 spike expression constructs with lentivirus packaging and helper plasmids into 293T cells using Fugene HD (Promega) as previously described 22 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Electroporated Halo-CENP-T was labelled using the Halo Tag Oregon Green fluorescent ligand (Promega) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... the MBP-tag was cleaved off the fusion proteins by ProTEV Plus (Promega, Cat# V6102). Cleavage efficiency was typically ~70-90% at 30 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... and (ii) HALO tag was incubated with primary Anti-HaloTag® Monoclonal Antibody (Promega #G9211) diluted 1:1 000 with secondary antibody Anti-Mouse IgG (Fc specific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with the Janelia Fluor 646 Halo tag ligand (JF646, 1μM, Promega #GA1120) in media at 30°C or 39°C for 7 min followed by washing (5X volume ...
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bioRxiv - Genomics 2023Quote: ... we followed a CRISPR knock-in protocol to endogenously insert a HiBiT tag (Promega 88) at the 3’ terminus of the IFNAR2-S mRNA (Fig ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized with primers specific for each strand separately at 50 °C (Supplemental Table S1) using the ImProm-II™ Reverse Transcription System (Promega, A3800) and subjected to qPCR with primers specific for the viral N-protein (Supplemental Table S1).
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were transfected with a PPRE-firefly luciferase reporter plasmid together with phRL-TK Renilla luciferase control vector (Promega, Madison, WI), pCMX-PPARα ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products amplified by gene-specific RT-qPCR primers listed in Supplemental Table S1 were cloned in pGEM-T Easy vector (Promega, Madison, Wisconsin, USA) prior to their sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... Barcodes were amplified using forward primer MWV 486 and a 5’-biotinylated reverse primer MWV 358 (Table S1) using standard PCR conditions with Gotaq G2 flexi DNA polymerase (Promega, Leiden, the Netherlands). The biotinylated PCR products were then hybridised to a pool of MagPlex-TAG microspheres (Table S3 ...
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bioRxiv - Biophysics 2023Quote: ... cells expressing mGluR3 with Halo tag in ICL2 were labeled with 1 µM CA-JF549 (Promega) and 3 µM CA-JF646 (Promega) ...
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120; Promega, Madison, WI, USA) for PCR amplification of a 535 bp region flanking the sgRNA cleavage site ...
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bioRxiv - Biochemistry 2021Quote: ... selections were performed using biotinylated c-CDCP1-Fc captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Biophysics 2023Quote: ... was generated by PCR-cloning the HaloTag coding sequence (primers details in Table S1, Integrated DNA Technologies) from pFN21K HaloTag® CMV Flexi® Vector (Promega, Madison, WI), followed by ligation in place of the mApple sequence in the mApple-CD36-C-10 vector (Addgene ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with cell-permeant TMR red Halo-tag ligand (1:2,000 for 15 min, Promega) 24 hours post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured via the HiBiT tag using Nano-Glo® HiBiT Extracellular Detection System (Promega) on a FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Biophysics 2023Quote: ... prior to incubation with the primary anti-Halo-Tag antibody (Mouse monoclonal antibody, Promega, Cat. No.: G9211). For incubation with the primary anti-RBPJ antibody (Rat monoclonal antibody ...
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bioRxiv - Immunology 2023Quote: ... stable HEK293 EBNA cell lines were generated using the sleeping beauty transposon system.91 Expression constructs were co-transfected with a transposase plasmid (10:1) into the HEK293 EBNA cells using FuGENE® HD transfection reagent (Promega GmbH, Madison, USA) in DMEM/F12 supplemented with 6% FBS ...
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bioRxiv - Biochemistry 2019Quote: ... The N-terminal 312 aa Halo tag sequence was PCR amplified from His6HaloTag® T7 Vector pH6HTN (Promega) as a 5’ SpeI ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Microbiology 2022Quote: ... The GFP and mEOS2 tags were amplified by PCR from peGFP and pmEOS2 using Pfu Polymerase (M7741, Promega), dNTP mix (10297018 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with 0.7 nM Janelia fluor 646 dye conjugated to Halo-tag ligand (JF646; Promega) in the dark at 30°C for 15 min and washed with plain PYE to remove excess dye ...
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bioRxiv - Cell Biology 2023Quote: ... The Halo tag sequence was amplified from the pFC20A (HaloTag®) T7 SP6 Flexi® vector (Promega, USA). All amplified fragments were then combined using Gibson assembly (Gibson assembly cloning kit ...
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bioRxiv - Biochemistry 2022Quote: ... The scFv gene was then ligated into pCSE2.6-hIgG1-Fc-XP vector (43) using T4 Ligase (Promega) and transformed into E ...