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Citations for Promega :
1 - 50 of 234 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Molecular Biology 2021Quote: ... S1 nuclease (Promega) or an equivalent amount of 1x S1 nuclease reaction buffer was added ...
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bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... S1 nuclease (M5761, Promega), Mung Bean nuclease (M4311 ...
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bioRxiv - Microbiology 2023Quote: ... and random 9-mers in the presence of RNasin (Promega). qPCR was then performed on a Quantstudio 3 using the indicated targets and with iTaq Universal SYBR Green (BioRad) ...
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bioRxiv - Bioengineering 2019Quote: For S1 Nuclease (Promega, Cat. # M5761) digestion ...
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bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
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bioRxiv - Molecular Biology 2023Quote: ... 1ng Luciferase Spike-In RNA (Promega), and 1X protease inhibitor cocktail ...
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... or HEK293 cells using Fugene 6 (Promega) following manufacturer’s instructions and studied 48-72 hours post-transfection.
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bioRxiv - Developmental Biology 2022Quote: ... and Klf4 vectors were co-transfected with pCL-Eco (Addgene ID 12371)149 in HEK293 cells with FuGENE6 (Promega) using low volume transfection protocol (Steffen et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... spike-in λ-DNA (Promega, Cat D150A) was added at a mass ratio of 1/200 ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were transfected with Fugene 6 (Promega). All other cell lines were transfected using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293 using FuGENE 6 transfection reagent (Promega). Culture supernatant containing lentiviral particles was harvested after 24-48h incubation and passed through a 0.45 μm filter ...
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bioRxiv - Microbiology 2021Quote: ... anti-Halo-tag (Promega), anti-FLAG (M2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Halo-Tag from Promega, USA (G9211) ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Microbiology 2022Quote: ... and a spike-expressing plasmid (pCAGGS-SARS-CoV-2-spike) were co-transfected into HEK293T cells using Fugene HD transfection reagentia (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with an GFP expression vector together with either a control expression vector (no spike) or a SARS-CoV-2 spike expression vector using Fugene (Promega). Two hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: HEK293 autophagy reporter cells (HiBiT-HaloTag-LC3, Promega #GA1040) were grown in in DMEM (ThermoFisher #31053-028 ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 were transiently transfected using Fugene HD (Promega, U.K.) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells were transiently transfected using Fugene 6 (Promega) with GFP-Tnf 3’UTR reporter constructs and a pGL3-mCherry control construct ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-Tag (Promega, G9211, mouse), and HSP90 (Santa Cruz Biotechnologies ...
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bioRxiv - Plant Biology 2024Quote: ... Magne Halo Tag Beads (Promega) were used to purify and capture the produced protein ...
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bioRxiv - Developmental Biology 2021Quote: ... Spike-in RNA (polyadenylated kanamycin mRNA #C1381, Promega, USA) was added to the Cell Lysis Buffer with a final concentration 107 molecules/ml ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 cells cotransfected with pFN21A HaloTag CMV Flexi vector (Promega) expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... an anchor-oligo(dT) primer (Table S1) and random hexamers (Promega). The reverse anchor and forward nested degenerated PGM primers derived from the oyster Crassostrea gigas and human Pgm-1 sequences were then used to perform the upstream amplification of cDNA fragments (see Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... The probes (Table S1) were 32P-labeled with T4 PNK (Promega) and purified using Oligo Clean and Concentrator columns (OCC-5 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with S1 nuclease (20 U/ml) (Promega M5761) in S1 buffer for 60 min at 37 °C
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... An N-terminal HALO tag (Promega) for fluorescent labelling was fused to the coding sequence through a GGSGGSLQ linker ...
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bioRxiv - Cell Biology 2022Quote: ... into pHTN Halo-Tag vector (Promega). mCherry-WT-p62 ...
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bioRxiv - Molecular Biology 2023Quote: ... Halo Tag Alexa Fluor 660 (Promega) was used ...
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bioRxiv - Cell Biology 2021Quote: HEK293 stably expressing pcDNA3.1(+)_SSF-GCGR cells transfected with GloSensor20F (Promega) were lifted and resuspended in imaging media (DMEM without phenol red supplemented with 30 mM HEPES ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were co-transfected using Fugene 6 transfection reagent (Promega) with MAC-tag (600ng ...
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bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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bioRxiv - Cell Biology 2020Quote: ... Expression plasmids (Table S1) were transiently transfected using Fugene6 (Promega, Madison, WI) according to the manufacture’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40- pGEM-T Easy and MERS-CoV UpE-pGEM-T Easy were first linearized by Sal I enzyme (Promega) creating a 5’overhang ...
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bioRxiv - Immunology 2021Quote: ... and HHEX (NM_002729) attached to a FLAG tag at the 5’ end and HiBit tag (Promega, Madison, WI) at the 3’ end ...
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bioRxiv - Developmental Biology 2021Quote: ... purified genomic DNA (with 1% unmethylated lambda DNA spike-in, Promega) was sheared to a fragment size of 100 - 700 bp (primary size 250 bp) ...
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bioRxiv - Biophysics 2021Quote: ... and Halo-Tag (O4) ligand (Promega, P6741). To avoid non-specific interaction the glass chambers were washed with blocking buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... JF646-conjugated Halo Tag ligand (Promega, GA1120) was added to culture at a final concentration of 200 nM and cells were incubated for 30 mins before live-imaging ...
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bioRxiv - Biophysics 2022Quote: ... Then Halo-Tag (O4) ligand (Promega, P6741) were passed and incubated overnight at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... Halo Tag Alexa Fluor 488 Ligand (Promega) was diluted with moderate amount of PBS ...
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bioRxiv - Microbiology 2023Quote: ... Mouse anti-HiBiT tag mAb (30E5, Promega) and goat anti-mouse IgG H&L conjugated with Alexa Fluor 568 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were rinsed and lysed with Passive Lysis Buffer (PBL, Promega), protein concentrations determined using the DC protein assay (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... retroviral plasmid was transfected to HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
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bioRxiv - Molecular Biology 2022Quote: HCT116 cells were transfected using JetPrime(Polyplus) and HEK293 using Fugene (Promega) using a standard protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmid DNAs were transfected into HEK293 cells with ViaFect Transfection Reagent (Promega) according to manufacturer’s instruction.