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Citations for Promega :
151 - 200 of 1553 citations for 6 Methyl 5 propyl 4 1H pyrimidinone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were developed with a commercial solution of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) according to the manufacturer (Promega, Madison, WI), in alkaline developing solution ...
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bioRxiv - Bioengineering 2024Quote: ... 10μM of 2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one (Ly294002: Promega, #V120A), 50ng/ml of Activin A (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell numbers were quantified using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... washed again in DPBS and stained with 1mg/ml X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside, Promega, Madison, WI, US) in DMF (Dimetil formamide ...
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bioRxiv - Immunology 2020Quote: Activity of the inflammatory caspases 1/4/5 was measured using a commercially available Caspase-Glo® 1 Inflammasome Assay (Promega, WI, USA) from HFFs seeded in 96-well plates (2×104 cells per well) ...
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bioRxiv - Biochemistry 2023Quote: ... MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (#G3581) was purchased from Promega (Charbonnières-les-Bains, France).
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bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were seeded in a 6-well plate and transfected with 750 ng of each of the AT1R-TANGO plasmids using FUGENE 6 Transfection (PROMEGA). The following day ...
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bioRxiv - Immunology 2020Quote: ... Plasmid transfections of IFITM1 3’ UTR reporter plasmids (500 ng per single well of a 6-well plate) were performed using the FuGENE 6 (Promega), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μg of total DNA (1:10 ratio of the Cre plasmid to the fusion protein plasmid) were mixed with 6 μl of Fugene 6 transfection reagent (Promega) in 100 μl of Opti-MEM ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed twice with TPBS and incubated for 1h at room temperature with the secondary antibody conjugated with horseradish peroxidase (Promega, #W401B, 1:5,000 dilution) followed by three more washes ...
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bioRxiv - Cell Biology 2020Quote: ... Transfections were carried out using Fugene 6 (Promega) at a ratio of 3µl reagent per µg DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... for retroviruses using 20 µl FuGENE 6 (Promega) in 400 µl DMEM per plate ...
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bioRxiv - Cell Biology 2020Quote: Plasmid transfection was performed using FuGENE 6 (Promega) according to the manufacturers’ protocol 24 hours before experiments whereas HiPerfect (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... plasmids using Fugene 6 Transfection Reagent (Promega, #E2311). 72 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... Cells were treated with 6 μM EnduRen (Promega), a substrate for Renilla luciferase (RL) ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected using FuGene-6 (Promega #E2692) with the following constructs ...
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bioRxiv - Genomics 2023Quote: hCNCCs were transfected with FuGENE 6 (Promega, E2691) immediately after passaging ...
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bioRxiv - Molecular Biology 2022Quote: ... H9 hESCs were transfected using FuGENE 6 (Promega) with a targeting construct containing Blasticidin selection cassette ...
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bioRxiv - Cell Biology 2022Quote: The Fugene 6 transfection reagent (Promega, WI, USA) was used to transfect WT and mutant E and 3a plasmids for patch-clamp ...
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bioRxiv - Biochemistry 2024Quote: ... Fugene 6 was purchased from Promega (Madison, WI). Multiple plasmids were ordered or generated for this study ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were transfected using FuGENE 6 (Promega) with complementary DNA (cDNA ...
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bioRxiv - Microbiology 2023Quote: ... 6 μL S30 premix without amino acids (Promega), plus water to a final reaction volume of 15 μL were incubated for 1 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... using the FuGENE®6 transfection reagent (Promega), and virus particles were harvested after 7 d of infection ...
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293 using FuGENE 6 transfection reagent (Promega). Culture supernatant containing lentiviral particles was harvested after 24-48h incubation and passed through a 0.45 μm filter ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were transfected with Fugene 6 (Promega). All other cell lines were transfected using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... the FuGENE 6 transfection reagent (Promega, WI, USA) was used to transfect wild-type (WT ...
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bioRxiv - Molecular Biology 2024Quote: ... hESCs were transfected using Fugene 6 (Promega, E2691) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 6 µL FuGENE HD (Promega, Walldorf, Germany) transfection reagent ...
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bioRxiv - Systems Biology 2024Quote: ... 6 µg of Trypsin Gold (Promega, Cat#V5280) were added to each sample (1 µg Trypsin ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was achieved by adding 6 μg of plasmid DNA in 18 microliters of FuGENE® 6 Transfection Reagent (Promega # E2691); the obtained mixture was incubated in plain media for 20 minutes and then added to the pre-seeded culture dishes ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 24-well plate or 6-well plate and transfected next day using FuGENE® 6 (Promega, E2691). For luciferase assay ...
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bioRxiv - Immunology 2020Quote: ... After a migration time of 4 h at 37°C (5 % CO2) lower compartment were analyzed for cell content by Cell-Titer-Glo® assay (Promega, Madison, WI, USA). Percentages of migrated cells were calculated as described before45 and normalized to the CXCL12-only control.
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bioRxiv - Molecular Biology 2024Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium (MTS) assay (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega, Madison, WI, USA). Ramos cells were seeded in 96-well plates at density of 105 cells/well and incubated for 48 h prior to experimental treatments ...
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bioRxiv - Genomics 2020Quote: ... 4 µL RNase-A (4 mg-mL-1, Promega, Madison WI) was incubated at 37 °C for one hour vortexing every 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly 70-90% confluent cells in 6-well plated were transfected with 1 µg CRISPR/Cas9 plasmids per well by using FuGENE 6 (Promega, Madison, Wisconsin). After 24 h post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with 36 µL FuGENE 6 (Promega) and 12 µg of retroviral plasmid DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... Transfection was performed with FuGENE 6 transfection reagent (Promega). All the cell lines were regularly confirmed to be free of contamination (e.g ...
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bioRxiv - Molecular Biology 2021Quote: HEK-293T cells were transfected using Fugene 6 (Promega), Lipofectamine LTX ...
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bioRxiv - Biochemistry 2020Quote: ... and 6 ng/μl of sequencing grade trypsin (Promega) for 1 h at 50 °C ...
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bioRxiv - Cell Biology 2021Quote: ... pKIF1CP176L-GFP or pKIF1CR169W-GFP using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Cell Biology 2021Quote: hTert-RPE1 cells were transfected using FuGENE 6 (Promega) according to the manufacturer’s instructions at a ratio of 1:3 ...
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bioRxiv - Cancer Biology 2021Quote: ... or the FuGENE 6™ (Promega, Fitchburg, WI, USA) transfection reagents according to the suppliers’ instructions for 1-2 days before the experiments ...
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bioRxiv - Neuroscience 2022Quote: ... then 6 μL FuGENE HD Transfection Reagent (Promega, USA) was added and vortexed to obtain a 3:1 reagent-to-DNA ratio ...
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bioRxiv - Immunology 2022Quote: ... by transfection using Fugene 6 (Promega, catalog no. E2692) and a combination of S plasmid ...
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bioRxiv - Immunology 2022Quote: ... and p MD.2G using Fugene 6 (Promega, PAE2693) in HEK293T cells ...
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bioRxiv - Genetics 2022Quote: ... The transfection mix was prepared using Fugene 6 (Promega) at 3µl/ug DNA in 500µl (total volume ...
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bioRxiv - Bioengineering 2020Quote: ... and 42 μL FuGENE 6 (Promega, Cat. No. E2691). The tube was gently flicked to mix the plasmids before and after the addition of FuGENE 6 ...