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Citations for Promega :
1851 - 1900 of 5396 citations for Rat Protein Glutamine Gamma Glutamyltransferase 2 TGM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... dithiothreitol was added to a final concentration of 50 mM and proteins were digested overnight with sequencing grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:50 at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Plant Biology 2021Quote: ... Halo fusion proteins were produced from 1.5 µg plasmid DNA in a 15 µL reaction using TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega). Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using FASP methods [24] ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted 10-fold in 50 mM Tris-HCl and digested with Sequencing Grade Modified Trypsin (Promega - V5111) at a Protein:Trypsin ratio 50:1 overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2M urea and digested with trypsin (Promega, UK), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of HiBiT proteins in the concentrated EV fraction was measured using the Nano Glo HiBiT Lytic Detection System (Promega). EV marker proteins in the concentrated EV fraction were detected by western blotting as described above.
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 150 ng trypsin (Trypsin Gold, Promega) for 90 min at room temperature in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... Remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 200 ng trypsin (Trypsin Gold, Promega) at 37 °C ON ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Biochemistry 2022Quote: Radiolabeled proteins were synthesized in the presence of 35S-methionine and 35S-cysteine using either coupled reticulocyte lysate system (TNT, Promega) or uncoupled reticulocyte lysate system (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... The Halo fusion proteins were produced using 1.2 μg plasmid DNA of the respective pIX-HALO construct in a volume of 15 μl using the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega). Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... The trapped proteins were washed four times with the methanol TEAB buffer and then digested using 1 µg Trypsin (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells expressing the auxin-responsive F-box protein Oryza sativa TIR1 (OsTIR1) under the control of a Tet promoter were transfected using FuGENE HD (Promega) with a CRISPR/Cas9 plasmid targeting nearby the 1st ATG codon of the RIF1 gene (5’-TCTCCAACAGCGGCGCGAGGggg-3’ ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Bone Morphogenetic Protein 4 (BMP4, 10 ng/ml, Bio-Techne) plus phosphoinositide 3-kinase inhibitor Ly294002 (10 µM, Promega). After 42 h incubation at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
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bioRxiv - Biochemistry 2019Quote: ... in vitro translated hnRNP M protein fragments (5 μl) produced by rabbit reticulocyte lysate using TNT Quick Coupled Transcription/Translation Systems (Promega). Proteins were incubated at 4°C for 2 hours in the binding buffer above ...
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bioRxiv - Molecular Biology 2021Quote: ... PGC-1α full-length and protein domain were translated in vitro in the T7 transcription and translation coupled reticulocyte system (Promega) supplemented with 35S-Methionine ...
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bioRxiv - Neuroscience 2019Quote: ... Immuno-precipitated proteins were released from the resin by on-beads digestion for 15min at 37°C using 200ng of trypsin (Promega). The supernatants were collected and subjected to conventional in-solution tryptic digestion (overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega). The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega) ...
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bioRxiv - Plant Biology 2020Quote: ... ZCCHC8B and RBM7 proteins were obtained by in vitro transcription and translation using the TNT T7 coupled wheat germ extract system (Promega) following the protocol supplied by the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: The linear dynamic range for measurement of complex peptide mixtures on the Lunatic was determined on a group of eight solutions prepared from a 1.0 μg/μl stock of a commercial yeast protein digest (Promega, V7461), dissolved in loading solvent (0.1% TFA in acetonitrile/water ...
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bioRxiv - Cancer Biology 2020Quote: Selected protein spots were excised from the gel and enzymatically digested in-gel essentially as previously described(4) using trypsin (Promega). Gel pieces were washed with 50% acetonitrile to remove SDS ...
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bioRxiv - Molecular Biology 2020Quote: ... The HaloTag-CLA4 fusion protein was expressed using the TNT® Coupled Wheat Germ Extract Systems (Promega, Fitchburg, WI, USA) and Magne® HaloTag Beads (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... v/v) and dehydration in ACN, proteins were digested overnight at 37 °C with trypsin (1:50, w/w) (V5280, Promega). Peptides were extracted from the gel in 50% ACN/0.1% formic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were digested either with trypsin in a trypsin/protein ratio of 1/50 (w/w) (Promega Cat. No. V5111) and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: Urinary proteins (100 μg of each sample) were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins on the filter unit were digested with 40 μl of trypsin solution (trypsin [V5111, Promega] in 50 mM NH4HCO3) at 37°C for 18 h ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was labeled with either 1.75 µM AlexaFluor 660 or 2.5 µM TMR HaloTag Ligands (Promega, G8471 and G8251) for 2 hr on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were on-bead digested using 5 μl of Sequencing Grade Trypsin (100 ng/μl in 10 mM HCl, Promega). The digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were rinsed with urea and ammonium bicarbonate solutions and digested overnight at 37°C using trypsin gold (Promega) at a ratio of 1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The 90% buffer was removed and protoplasts were flash-frozen in liquid nitrogen to quench the protein degradation process followed by luciferase activity assay (E1500, Promega) to determine the relative amount of RAP2.12(1-28)-luciferase chimera protein remaining at various time points ...
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bioRxiv - Developmental Biology 2022Quote: A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Molecular Biology 2022Quote: ... a total of 225 μg of protein extract isolated using the aforementioned hypotonic buffer (additionally supplemented with 100 U/ml RNasin Plus RNase Inhibitor (Promega) and 2 U/mL Turbo DNase (Ambion) ...
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bioRxiv - Plant Biology 2022Quote: ... were reduced with DTT and then alkylated with iodoacetamide and digested overnight using Lys-C/Trypsin (1:50, enzyme to protein; Promega). After terminating the digestion with 1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2023Quote: ... the samples were digested by trypsin with 1/80 (w/w) trypsin/total protein ratio (Sequencing Grade Modified Trypsin, Promega) according to the manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega, G9211) and anti-SBPTag mouse anti-human antibody (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein precipitate was air-dried and resuspended in 200ul of 25mM ammonium bicarbonate solution and 3ul of trypsin (Promega) and digested overnight at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Wells containing single cell colonies were duplicated into 12-well plates and screened for HiBit expression by mixing crude lysates with purified LgBit protein (Promega) and measuring luminescence in the presence of 5 μM furimazine ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellets were resuspended in 50 μl 100 mM TEAB and digested with 200 ng trypsin (Promega sequencing grade) overnight at 37°C.
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...