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Citations for Promega :
1651 - 1700 of 5396 citations for Rat Protein Glutamine Gamma Glutamyltransferase 2 TGM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Digested proteins were finally desalted using a C18 TopTip (PolyLC ...
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1x ProTEV digestion buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Finally ...
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bioRxiv - Cell Biology 2019Quote: Target proteins were in vitro transcribed and translated in rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with 35S-Methionine (IsoLabel L-35S Steady Blue ...
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... The solution was diluted to below 1M Urea using 200mM bicarbonate buffer and an aliquot of approximately 25μg protein were digested using sequencing grade Trypsin (V511A, Promega) at 37°C over-night (Trypsin to protein ratio of approx ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Biochemistry 2020Quote: ... Protein (100 μg) digestion was performed by the FASP method (Tang et al., 2017) utilizing trypsin (Promega, Madison, WI) and 0.1M tetraethylammonium bromide (TEAB ...
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bioRxiv - Cancer Biology 2020Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 0.2 mg of protein was subjected to overnight digestion with 8.0 µg of mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were precipitated with 0.1 M ammonium acetate in 100% methanol and the protein pellets were further digested with sequencing-grade trypsin (Promega). For the analysis involving the rpsAΔ6 mutant missing the last C-terminal OB fold domain of protein S1 ...
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bioRxiv - Microbiology 2021Quote: ... Following the dilution of the sample with 100 mM ammonium bicarbonate to a final urea concentration of 800 mM the proteins were digested further with 0.2 µg/µl trypsin (Promega) (37 °C ...
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bioRxiv - Microbiology 2021Quote: ... The SK-N-SH cell surface proteins with attached TBEV virions were digested off with 1.25 μg trypsin (Promega) and the supernatant collected ...
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bioRxiv - Microbiology 2021Quote: ... 0.025% deoxycholate and 200 µg.mL−1 RNase A at 37°C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin-bound proteins were solubilised by incubation with 1320 U of DNase I (RQ1 RNase-free DNase I, Promega) on ice for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The proteins in the sample were digested with 10 μg/mL sequence grade modified trypsin (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Neuroscience 2020Quote: A total of 100 μg protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation methods [19] ...
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bioRxiv - Genetics 2022Quote: ... Protein-bound beads were washed in 80% ethanol and proteins were digested off the beads by addition of 0.8ug sequencing grade modified trypsin (Promega) in 100mM Ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2022Quote: ... IAA was neutralized by adding 10mM DTT final concentration and proteins were digested with 300ng of trypsin (Promega V5280) for 18h ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). The digested proteins were desalted using AssayMap C18 cartridges mounted on a BRAVO liquid handling system (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
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bioRxiv - Cell Biology 2019Quote: ... the protein sample was digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Indicated amounts of HMGA2 and mutant HMGA2 protein were incubated with 100ng of negatively supercoiled Renilla reporter plasmid (Promega) for 30mins at 37°C in a buffer containing 10mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... In-gel protein digestion was performed overnight at 37°C with trypsin (Sequencing Grade Modified Trypsin, Promega, Madison, USA) at a final concentration of 0.005 µg/µL ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were obtained from in vitro coupled transcription/translation systems (TNT® T7 Coupled Reticulocyte Lysate Systems, Promega). 15 µl of Flag-tagged proteins from TNT assays were pre-incubated on ice for 10 minutes in modified DBB (DBB ...
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bioRxiv - Cancer Biology 2020Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega) overnight at 37°C ...
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bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
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bioRxiv - Plant Biology 2022Quote: ... ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bands corresponding to the protein of interest were cut from the gel and in-gel digestion with trypsin (Promega) was performed essentially as described in 41 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were rinsed using 80% EtOH and proteins were digested with 100 µL of trypsin in 50 mM TEAB pH 7.55 (Promega, protein:enzyme ratio 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... Unlabeled proteins were cell-free-synthesized using the TNT T7 Quick Reaction system according to the manufacturer instructions (Promega) and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... proteins that underwent in vitro reactions were digested in 50 mM ammonium bicarbonate (NH4HCO3) with 0.2 µg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... and αKNL2-CΔDNAb (1,2) fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0 to an urea concentration of 4 M and proteins were digested with 40 µg endopeptidase LysC (Promega) (1/250 ...
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bioRxiv - Plant Biology 2019Quote: ... using 1 - 2 µg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Genetics 2020Quote: ... 2 µg of DNA in 50 µl of DMEM and 50 µl of FuGene6 transfection reagent (Promega) at 1mg·ml-1 in DMEM were incubated separately for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with 1 – 2 μg EphA2-mTurquoise plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...