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Citations for Promega :
1801 - 1850 of 4877 citations for Human Gap Junction Alpha 8 Protein CX50 GJA8 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Peptide mass spectrometry was completed after reduction with 10 mM DTT and alkylation with 55 mM iodoacetamide and protein digestion overnight at 37 °C at a 1:50 ratio of trypsin (Promega, UK). Tryptic peptides were analysed by nano-scale capillary LC-MS/MS with an Ultimate U3000 HPLC (ThermoFisher Scientific Dionex ...
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bioRxiv - Neuroscience 2021Quote: ... Further 50ug in 100 µl protein sample was added into vials and for ATP measurement luminescence was measured through luminometer (Promega glowmax).
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bioRxiv - Cell Biology 2021Quote: ... and 40 μg of proteins/sample was loaded onto S-trap columns (Protifi) and digested overnight with 1 μg sequencing grade modified trypsin (Promega, V5111) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Physiology 2022Quote: ... In-gel digestion of proteins was performed overnight at 37°C using trypsin (Promega Madison, WI, USA; 40 ng per band), and the resulting peptides were extracted twice (2 x 45 min ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 1 M with 50 mM ABC and the proteins digested with trypsin (Promega, V511c) at 1:100 mass-ratio at 37 °C overnight.
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bioRxiv - Immunology 2022Quote: ... Samples were diluted two-fold with 100 mM of triethylammonium bicarbonate (TEAB) and proteins were digested during 3 hours with 1 µg of trypsin (Promega V5111) and 1 µg of LysC (Wako 129-02541 ...
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bioRxiv - Cancer Biology 2022Quote: ... subjected to SP3 protein clean up as previously described (87, 88) and eluted in AmBic 50mM prior digestion with 300ng trypsin (Promega V5280). For the on-chromatin interactors ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... mRNA for each chimeric Lep protein was produced from plasmids using the RiboMAX RNA production system in accordance with the manufacturer’s instructions (Promega, Madison, WI). Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: All wheat germ cell-free expression used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Myc tagged full-length and truncated ARL13B fragments were generated by in vitro translation using TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30 min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37° C for 12 hours ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
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bioRxiv - Plant Biology 2022Quote: ... PtaMYC2.2 and PtaJAM1.1 were cloned into pIXHALO (ABRC, Ohio State University) and expressed in vitro using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). We used empty vector pIXHALO as control ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration in the supernatant was measured using a NanoDrop and 1:200 (w/w) of trypsin (Promega, Mass Spectrometry grade) was added to 200 µg of sample ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assessment of total cellular HiBiT-GCase protein abundance was performed using the Nano-Glo® HiBiT Lytic Detection System (Cat.#N3040, Promega), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega, cat. no. L4140) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant (700 μL) was transferred to a sterile Eppendorf tube and mixed with 50 μL protein precipitation solution (Promega, USA) by inverting it ten times before centrifugation (10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Then, the proteins were purified by acetone precipitation and digested with LysC (FUJIFILM Wako, Osaka, Japan) and trypsin (Promega, Madison, WI). The resulting peptides were desalted on InertSep RP-C18 columns (GL Sciences ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The quantity of the reporter protein was examined by measuring its enzymatic activity through the Renilla Luciferase Assay System (Promega, Germany), as per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Biochemistry 2023Quote: The FASP method[7] was used to digest urine protein with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA). One hundred micrograms of urine protein was added in the membrane of a 10KD ultrafiltration tube (Pall ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method with Trypsin/Lys-C mix (Promega, USA). Next ...
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bioRxiv - Microbiology 2024Quote: ... the protein pellets were resuspended in 100 µL 0.6M urea in 100mM TEAB buffer and 4 µg trypsin (Promega, Cat#V511B) was added for digestion at 1:25 enzyme:protein at 500 rpm at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... The FLAG-tagged VRS5 and GFP were expressed in-vitro using 2 µg plasmid and TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). Expressed FLAG-tagged proteins were bound with prepared DNA libraries for 2 hours at RT followed by immobilization by anti-FLAG magnetic beads (SIGMA) ...
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bioRxiv - Molecular Biology 2024Quote: ... The band corresponding to streptavidin was discarded and the remaining proteins were digested in-gel using modified trypsin (Promega, sequencing grade) as previously described (51) ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro expression of Halo-BnC07MYB3a fusion proteins was performed using the TNT SP6 Coupled Wheat Germ Extract System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The iodoacetamide was discarded and the gel bound proteins were digested with 0.5 ml of 20 ng/µl trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate buffer at 37 °C with constant mixing for 12 h ...
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bioRxiv - Immunology 2024Quote: ... Beads were resuspended in ammonium bicarbonate and proteins digested on the same day using 0.5 ug Sequencing Grade Modified Trypsin (Promega, Madison, WI) overnight at 37°C with agitation ...
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bioRxiv - Genomics 2024Quote: ... A total of 10 µL at a minimum concentration of approximately 100 ng/µL of each PCR amplicon was used to express TF proteins in vitro using the TnT T7 Quick for PCR DNA System (Promega L5540) with an incubation at 30⁰C for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Cloning of the mNeonGreen fused shelterin proteins was performed by Gibson assembly into pRK2 backbone (original pHTN HaloTag CMV-neo, Promega #G7721).
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bioRxiv - Neuroscience 2021Quote: ... using CalPhos™ Mammalian Transfection Kit (Takara Bio or Promega) on DIV7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were cloned using the pGEMT kit (Promega): 3 μl PCR products were ligated to 50 ng pGEMT vector in the presence of 3 units T4 ligase overnight at 18°C (final volume 10 μL) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Locusta cDNA was synthesized using the cDNA Synthesis Kit (Promega). 2.5 ng of cDNA was used in each qPCR reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... An ImProm-II™ Reverse Transcriptase System kit (Promega, USA) was used to synthesize the first strand cDNA using 1.5 μg of the DNA-free RNA sample as a template and each reaction was carried out according to the supplier’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA was prepared using Promega kit (REF 017319, Promega, USA). Primers were designed according to the sequence information from the NCBI database using Primer3 Input (version V ...
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bioRxiv - Genomics 2020Quote: ... Assays were performed using the dualluciferase reporter assay kit (Promega) and a Veritas Microplate Luminometer (Turner Biosystems) ...
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bioRxiv - Immunology 2021Quote: The T Cell Activation Bioassay kit was purchased from Promega. The Jurkat-NFAT cells were thawed and cultured in RPMI (supplemented with 10% FBS and 5% pen/strep ...
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bioRxiv - Immunology 2021Quote: ... cDNA was then synthesized using AMV reverse transcriptase kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using the Dual-Glo Luciferase Assay System Kit (Promega, US) as substrate ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then synthesized using AMV reverse transcriptase kit (Promega) with Oligo-dT primer ...
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bioRxiv - Bioengineering 2021Quote: ... and collagen content via a QuantiFlour dsDNA assay kit (Promega), a modified 1,9-dimethylmethylene blue (Sigma-Aldrich ...