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Citations for Promega :
1801 - 1850 of 2468 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CytoTox-OneTM homogeneous membrane integrity assay kit and Caspase-Glo® 3/7 assay system kit were from Promega, WI ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (M1705, Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Immunology 2022Quote: 5 million THP-1 expressing HA-Ubiquin (from pTRIP-SFFV-Blast-2A-HA-Ubiquitin) were plated in 5ml of fresh medium containing 5µg/ml digitonin (Promega) per well in a 6 well plate the day of the stimulation ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA from peripheral blood leukocytes was extracted using the Maxwell® RSC automatic extractor (Promega, Madison, Wisconsin, WI, USA), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... twice and 20 µL of a 1:19 mixture of substrate:buffer from the Nano-Glo Live Cell Assay (Promega) were added to 80 µL of OptiMEM per well ...
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bioRxiv - Molecular Biology 2022Quote: The plasmid pEGFP-C1-human Rab29 was generated by inserting human Rab29 sequence from pFN21A-Halo-Rab29 (Promega, #FHC08084) into Bgl II - Eco RI site of pEGFP-C1-rat Rab29 plasmid that was used previously11 ...
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bioRxiv - Biochemistry 2022Quote: ... containing NanoLuc substrate was added to the living cells (40 µl/well, containing 1.0 µl of NanoLuc substrate stock from Promega). Thereafter ...
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bioRxiv - Cell Biology 2022Quote: ... pX459-derived plasmids encoding both Cas9 and the gRNA were transfected using Fugene 6 (cat. no. E2692 from Promega). 24 h post-transfection puromycin was used to select for transfected cells ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Genomics 2022Quote: ... Single nuclei were sorted into 384 well plates containing 1 µl M-Digestion Buffer containing proteinase K and ~0.05 pg Lambda DNA isolated from dcm+ E.Coli (Promega #D1501).
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bioRxiv - Microbiology 2022Quote: ... PCR amplification of the whole hsdS locus (3.7 kb) was carried out from extracted genomic DNA using GoTaq DNA polymerase (Promega) with primers App_T1_For_FAM and App_T1_Rev (Supplementary Table S3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted from 293T cells using an Eastep Super Total RNA Extraction Kit (LS1040, Promega, WI, USA). Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800 ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from leaves or bacterial liquid cultures via phenol/chloroform extraction and treated with DNase 1 (Promega) to remove gDNA contamination ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNA was isolated from 50 μl of cell suspension with the Wizard SV Genomic DNA purification system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... LucN luminescence was detected from the same sample by adding 30 μL NanoDLR™ Stop & Glo® Reagent (Promega). After incubation for 10 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... gBlocks or eBlocks were synthesized (IDT) ranging from 150-400 bp in length and cloned into pGL4.26 (Promega #8441) for enhancer screening or CMV-Enh-Luc (Addgene #45968 ...
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bioRxiv - Developmental Biology 2023Quote: ... Ghost Database KY21.Chr1.1715) we extracted genomic DNA from local Norwegian animals using the Wizard Genomic DNA Purification Kit (A1120, Promega). We then used the purified gDNA at a concentration of 100-150ng/μl ...
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bioRxiv - Genomics 2023Quote: ... The platform also permits a high-throughput automated genomic DNA isolation from 48 samples in 40 minutes (Promega, 2020) making this system compatible with larger sample sets.
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bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was isolated from overnight cultures of Ag1 and Ag2 using the Genome Wizard kit (Promega, WI), following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Females from each progeny were then pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check the frequency of the dsxFΔ11 allele in the offspring ...
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bioRxiv - Developmental Biology 2024Quote: ... containing the promoter region of spia-1 (1.23 kb upstream from the start ATG) and the entire genomic region into the pGEMT vector (Promega). A transcriptional construct (pSO22) ...
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bioRxiv - Cell Biology 2024Quote: ... 20 μl per sample resulting from PCR were mixed with Blue/Orange loading buffer loading dye 6x (PROMEGA, G190A) and loaded onto gel ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The dye was released from cell inserts using 30% acetic acid and absorbance was measured using GloMax Explorer (Promega) microplate reader at 590 nm as a readout for cell migration.
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was prepared from 1 μg of total RNA using Random Primers and M-MLV Reverse Transcriptase (both Promega). Primers were allowed to bind to template RNA for 5 minutes at 70°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Aliquots (2.5 μL) from each reaction were added to a 50 μL mixture containing the luciferase assay reagent (E1483 Promega) in a luminometer microplate ...
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bioRxiv - Microbiology 2024Quote: ... Samples were prepared according to the directions from the Wizard HMW DNA Extraction kit for Gram-negative Bacteria (Promega).56 The genomic DNA was rehydrated in DNA Rehydration Solution and Illumina sequenced (SeqCoast) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The dissected tissues were directly collected in LB+TGA lysis buffer from Reliaprep RNA Tissue Miniprep System (Promega, USA), and homogenized using a Biomasher Standard homogenizer (Takara Bio Inc. ...
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bioRxiv - Microbiology 2024Quote: ... 35 uL of infected (and uninfected) cell culture supernatants were collected from each well and LDH quantified as per manufacturer’s instructions (Promega), with percent cytotoxicity calculated as a proportion of lysed control cells and blank media ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then transfected with pSG5 SV40 large T antigen plasmid (a gift from David Huang) with FuGeneHD (Promega). Cells were maintained in culture until their growth was stable and consistent ...
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bioRxiv - Molecular Biology 2024Quote: Both wt PACT and lear-5J constructs in pCDNA3.1-were in vitro translated using the TNT-T7-coupled rabbit reticulocyte system from Promega while incorporating an 35S-Methionine radiolabel and the dsRNA binding ability was measured using poly(I:C ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from each thrombus using a ReliaPrep RNA tissue MiniPrep System (Z6110, Promega, Madison, WI, USA) and from whole blood using a PAXgene Blood RNA Kit (762164 ...
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bioRxiv - Molecular Biology 2024Quote: ... The coding sequence of NanoLuc was amplified by PCR using the primers 5-NanoL and 3-NanoL from pNL1.1.CMV plasmid (Promega). The resulting fragment was digested with BamHI and NotI enzymes and cloned into pEGFP-N1 digested with the same enzymes ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse HARE5 fragments were amplified from genomic DNA of individual species and inserted into the pNL1.1 vector (Promega) by KpnI and EcoRV enzyme sites ...
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bioRxiv - Microbiology 2024Quote: Samples of Pst DC3000 subpopulations were collected from colonies grown on swimming medium using 0.13 x 0.33 inch gel extractors (x-tracta™, Promega) and resuspended in TriZol reagent (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and cDNA was synthesized using MMLV (M1705) and amplified with GoTaq qPCR Master Mix (A6002) from Promega (Hampshire, UK) with the primers listed previously [35] ...
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bioRxiv - Cell Biology 2024Quote: ... Tracer 8 (20X) was prepared from a 400 μM stock solution in DMSO with tracer dilution buffer (Promega, #N291B) and 20% DMSO ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 µL and 25 µL of the trypsin solution (100 ng µL-1 of trypsin sequencing grade from Promega) were added to EV and WC samples ...
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bioRxiv - Plant Biology 2024Quote: ... The coding sequence of the target genes were PCR-amplified from cDNA using the GoTaq Green Master Mix (Promega) and gene-specific primers (Supplementary Table 1).
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bioRxiv - Biochemistry 2024Quote: ... The DNA template was produced from a PCR reaction of pBS117 plasmid (23) using Taq DNA polymerase (M7122, Promega), followed by gel purification of the products with a Wizard SV Gel and PCR Clean-Up System kit (Promega) ...
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A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cellbioRxiv - Systems Biology 2024Quote: NanoLuciferase protein expression was measured from triplicate collections of EEEV FL93 nLuc infected cell lysates by the manufacturer’s protocol (Promega) as previously described in [152] on a Biotek Synergy LX plate reader.
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bioRxiv - Biochemistry 2024Quote: ... The 10 mM stock solution of UDP was taken from the UDP-Glo™ Glycosyltransferase Assay (Promega, Part #V698A).
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bioRxiv - Biophysics 2024Quote: ... The NF-κB luciferase reporter vector (pGL4.32-luc2P/NF-kappaB-RE/Hygro) and the Renilla luciferase reporter vector (pGL4.74-hRluc/TK) were purchased from Promega.
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bioRxiv - Cancer Biology 2024Quote: ... FGFR2-ACHYL1 and FGFR2-BICC1 were C- terminally tagged with Small BiT or Large BiT derived from NanoLuc (Promega). HEK293T cells were stably or transient transfected ...
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bioRxiv - Cell Biology 2024Quote: ... Reagents 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) were from Promega (Madison, WI). The inhibitors of protein synthesis cycloheximide (CXH) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the aqueous phase was collected in EtOH and RNA lysis buffer from the SV Total RNA Isolation kit (Promega), and the manual instruction was followed ...
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bioRxiv - Plant Biology 2020Quote: ... The template was obtained from shoot material extracted as described above and processed into cDNA by use of RQ1-DNase (Promega) and SuperScript III Reverse Trascriptase kit (Thermo-Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.