Labshake search
Citations for Promega :
1701 - 1750 of 2468 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... The total RNAs were isolated from the leaf tissues using SV Total RNA Isolation System (Promega, Madison, WI, USA). A thousand ng of total RNAs were used for cDNA synthesis using the SMART MMLV Reverse Transcriptase (Takara Bio ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Amplicons that showed positive cleavage from the T7 endonuclease Ⅰ assay were subcloned into the pGEM-Teasy vector (Promega) through TA cloning ...
-
bioRxiv - Developmental Biology 2020Quote: His-P150-CC1 (Courtois et al., 2012) was expressed in and purified from BL21(DE3)pLysS competent cells (Promega) as previously described (Courtois et al. ...
-
bioRxiv - Systems Biology 2022Quote: ... Sample concentrations were measured by incubating 1μl of cDNA from each well with 49μL of a fluorescent dsDNA dye (Quantifluor dsDNA kit, Promega E2670) and measured on a plate reader with fluorescent detectors (504nM Excitation/531nM Emission ...
-
bioRxiv - Microbiology 2022Quote: ... DNA from the saliva samples (n=67, one missing) was extracted using the ReliaPrep Blood gDNA Miniprep (Promega, USA). For microbiome profiling ...
-
bioRxiv - Molecular Biology 2020Quote: RNA from chicken samples was isolated by Reliaprep™ RNA Tissue Miniprep System according to manufacturer instructions (Promega, USA), followed by cDNA synthesis using GoScript Reverse transcription mix (Promega ...
-
bioRxiv - Physiology 2020Quote: ... Transfection efficiency was measured by normalization to Renilla luciferase activity expressed from a co-transfected pTK-RL vector (Promega).
-
bioRxiv - Genomics 2020Quote: ... The nitrite concentrations were detected in culture supernatants using Griess reaction and a commercial kit from Promega (WI, USA) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from high titer lysates using the Wizard® Clean-Up Kit (cat. # A7280, Promega, WI, USA). Sequencing libraries were constructed with the NEBNext® UltraTM II DNA Library Prep kit (New England Biolabs ...
-
bioRxiv - Bioengineering 2020Quote: ... the Nbs-virus mixture was removed from Vero E6 cells followed by 500 μL of DMEM with 2% FBS and 0.9% carboxymethyl cellulose (Promega) was overlaid ...
-
bioRxiv - Cancer Biology 2020Quote: ... The psiCheck2 reporter construct containing dual Renilla and Firefly luciferase reporters was purchased from Promega (Promega, Madison, WI, USA). The DDX5-bound sequences identified by eCLIP IDR were cloned into a multiple cloning site located downstream of the Renilla translational stop codon ...
-
bioRxiv - Cell Biology 2021Quote: ... The 4% PFA was removed from the samples with 3 washes of PBSTriton (0.1% Triton-X-100 (Promega: H5141) in 1x Phosphate Buffered Saline (PBS) ...
-
bioRxiv - Plant Biology 2020Quote: ... total RNA was extracted using Maxwell® RSC Plant RNA or Maxwell® 16 miRNA Tissue Kit from Promega according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... pH 8.0) for four hours at room temperature and digested in 0.4 µg/µL of trypsin (both purchased from Promega) overnight at room temperature ...
-
bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from cultures using the ReliaPrep gDNA tissue Miniprep system following the manufacturer’s guidelines (Promega, USA). This DNA was screened for Brucella DNA using Brucella specific primers (Table 1 ...
-
bioRxiv - Plant Biology 2021Quote: ... Undigested bands from the restriction enzyme digestion were purified and cloned into the pGEM-T easy vector (Promega, USA) or using Zero Blunt™ TOPO™ PCR Cloning Kit (Invitrogen™ ...
-
bioRxiv - Plant Biology 2020Quote: ... Absolute quantification was obtained with DNA fragment from the Map gene cloned in pGEM®-T Easy vector (Promega) with 10 to 108 copies of the plasmid added on each PCR plate ...
-
bioRxiv - Cell Biology 2021Quote: RNA extraction from FFPE specimens of chRCC was performed using the Maxwell® 16 Tissue DNA Purification Kit (Promega). RNA quality was assessed with the RNA Qubit™ RNA HS Assay Kit (ThermoFisher) ...
-
bioRxiv - Cell Biology 2022Quote: The S1PR1 3’UTR sequences and mutant sequences (200 bp upstream and 200 bp downstream of the binding site from NM_007901.5 transcript) were synthesized and cloned into wildtype plasmid pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega, USA) by Generay (China) ...
-
bioRxiv - Neuroscience 2022Quote: ... wherein the 3’ UTR of Prr7 (NM_0010302964) was amplified from mouse DNA and cloned into the pmiRGLO dual-luciferase expression vector (Promega). Mutations in miRNA binding sites conserved across mammals were introduced by site-directed mutagenesis using Pfu Plus! DNA Polymerase (Roboklon).
-
bioRxiv - Developmental Biology 2022Quote: ... Tails were dissected from the injected embryos at 23-24 hpf and lysed with passive lysis buffer (E1941, Promega) for subsequent Dual-Luciferase assay following the manufacturer’s instructions (E1910 ...
-
bioRxiv - Systems Biology 2022Quote: ... NanoBRET™ Nano-Glo® Detection System and CellTiter-Glo® Luminescent Cell Viability Assay was purchased from Promega.
-
bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
-
bioRxiv - Developmental Biology 2020Quote: ... The cDNA was synthesized from 1 μg of the total RNA using the ImProm-II Reverse Transcription System (Promega) and oligo (dT)23 primers.
-
bioRxiv - Genomics 2020Quote: DNA was extracted from a single colony of each isolate with a Wizard® Genomic DNA Purification Kit (Promega), and the quantity and quality were determined with a Quantus Fluorometer (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 400 ng of RNA using ImProm-II Reverse Transcription system kit and random primers (Promega). Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) ...
-
bioRxiv - Immunology 2020Quote: ... A total of 0.8 μg RNA from different treatments was reversely transcribed into cDNA using M-MLV reverse transcriptase (Promega) with an oligo (dT ...
-
bioRxiv - Neuroscience 2020Quote: ... The first strand cDNA was synthesized from 2 μg RNA with GoScript™ Reverse Transcriptase (Promega, Madison, WI, USA).
-
bioRxiv - Microbiology 2020Quote: ... Viral DNA was extracted from the concentrated and purified viral fraction using Wizard DNA Clean-Up System (Promega A7280) to remove PCR inhibitors (John et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
-
bioRxiv - Immunology 2021Quote: ... RNA was isolated from formalin-fixed and paraffin embedded tissue with the use of Maxwell RSC RNA FFPE (Promega) according to manufacturer recommendations ...
-
bioRxiv - Bioengineering 2020Quote: ... and an absorbance LDH assay (Lactate dehydrogenase assay, CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit from Promega, G7890) was used to determine the level of cytotoxicity caused by membrane compromisation ...
-
bioRxiv - Microbiology 2022Quote: ... First-strand cDNA generation from total RNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Relative mRNA quantitation was done using the SYBR green quantitative real-time PCR (qRT-PCR ...
-
bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
-
bioRxiv - Cell Biology 2020Quote: ... Reverse transcriptase reaction was performed from 2 µg of total RNA by using ImProm-IITM Reverse Transcription System (Promega). Aliquots of cDNA obtained were amplified in PCR and quantitative real-time PCR reactions ...
-
bioRxiv - Pathology 2020Quote: Cells isolated from the whole constructs were used for RNA isolation using ReliaPrepTM RNACell Miniprep System (Promega, Madison, WI) following manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and were frequently tested for absence of mycoplasma and cell lines were authenticated using STR profiling kit from Promega.
-
bioRxiv - Synthetic Biology 2020Quote: ... LucN luminescence was detected from the same sample by adding 30 μL NanoDLR™ Stop & Glo® Reagent (Promega). After incubation for 10 min at room temperature ...
-
bioRxiv - Zoology 2020Quote: ... Other sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
-
bioRxiv - Molecular Biology 2020Quote: gDNA samples for library preparation were extracted from overnight LB cultures using the Wizard Genomic DNA Purification kit (Promega) as described above ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells from each well were lysed using the Bright-Glo Luciferase Assay System (Cat#: E2610, Promega, Madison, WI, USA), and the cell lysates were transferred to 96-well Costar flat-bottom luminometer plates ...
-
bioRxiv - Immunology 2020Quote: ... was reverse transcribed from RNA extracts using GoScript reverse transcriptase with random primers according to the manufacturer’s instructions (Promega). qPCR reactions were carried out on a Stratagene Mx3005p thermal cycler (Agilent Technologies) ...
-
bioRxiv - Molecular Biology 2020Quote: ... ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A) (Promega). ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
-
bioRxiv - Microbiology 2020Quote: ... fLuc activity was measured using a luciferase assay system kit and GloMax® Navigator Microplate Luminometer (both from Promega).
-
bioRxiv - Microbiology 2021Quote: ... and the lentiviral packaging plasmids pVSV-G, pΔVPR, pAdvant (gifts from Dr. Jan Carette, Stanford University) using FuGENE HD transfection reagent (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: Cell pellets from trypsinised cells underwent RNA extraction with DNase digestion using Reliaprep™RNA Cell Miniprep System (Promega). RNA quantitation was performed using a NanoDrop Spectrophotometer (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... qPCR was then performed to detect viral gene ORF26 and luciferase from the carrier plasmid using GoTaq polymerase (Promega) and the ΔΔCT method ...
-
bioRxiv - Microbiology 2021Quote: ... seriolae gDNA was extracted from 5-day old cultures using the Wizard® Genomic DNA Purification Kit (Promega, USA) as per the manufacturer’s instructions ...