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Citations for Promega :
1651 - 1700 of 4516 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and immersed in 100 µL of growth medium and 20 µL of CellTiter 96 AQueousOne Solution Reagent (Promega Co.). After another incubation for 3 h at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Cell Biology 2020Quote: Proteins on beads were washed twice with 100 μL of 25 mM NH4HCO3 and we performed on-beads digestion with 0.2 μg of trypsine/LysC (Promega) for 1 h in 100 µL of 25 mM NH4HCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... and harvested at varying timepoints by resuspending the cells in 50-100 μL of 1X Passive Lysis Buffer (Promega). Inductions were staggered so that all timepoints could be harvested simultaneously ...
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bioRxiv - Biochemistry 2022Quote: ... The gel pieces were then first dehydrated with 100 % acetonitrile and then rehydrated with sequence grade trypsin solution (0.6 µg, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... beads were resuspended in 100 µL of 25 mM NH4HCO3 and digested by adding 0.2 µg of trypsin/LysC (Promega) for 1 h at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... cDNA was added as 100 ng/reaction to SYBR green based GoTaq® qPCR Master Mix (Promega, Wisconsin, USA). The primer sequence for CEBPa was F ...
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bioRxiv - Cell Biology 2019Quote: ... were transfected with 1 µg of DNA and 4 µL FuGENE 6 in 100 µL Opti-MEM media for 24-36 hours according to the manufacturer’s instructions (Promega). Cells were fixed with 4% (v/v ...
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bioRxiv - Systems Biology 2020Quote: Frozen fly brains were resuspended in 100 μL of lysis solution containing 0.1% (w/v) ProteaseMAX Surfactant (Promega, USA), 50 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2020Quote: ... Transfection was performed using 100 ng of expression plasmid using 0.3 μl of FuGENE HD Transfection Reagent (Promega E2311) in 20 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein (100 μg) digestion was performed by the FASP method (Tang et al., 2017) utilizing trypsin (Promega, Madison, WI) and 0.1M tetraethylammonium bromide (TEAB ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
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bioRxiv - Cell Biology 2021Quote: ... The 4% PFA was removed from the samples with 3 washes of PBSTriton (0.1% Triton-X-100 (Promega: H5141) in 1x Phosphate Buffered Saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... Dried reduced/alkylated samples were then rehydrated with 20 ng/µl trypsin in 100 mM NH4HCO3 (Promega, Madison WI) on ice for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were precipitated with 0.1 M ammonium acetate in 100% methanol and the protein pellets were further digested with sequencing-grade trypsin (Promega). For the analysis involving the rpsAΔ6 mutant missing the last C-terminal OB fold domain of protein S1 ...
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bioRxiv - Molecular Biology 2021Quote: Proteins on beads were washed twice with 100 μl of 25 mM NH4HCO3 and subjected to on-beads digestion with 0.2 μg of trypsine/LysC (Promega) for 60 min in 100 μl of 25 mM NH4HCO3 ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of guanidium hydrochloride was reduced to 0.5 M by dilution with 100 mM ammonium bicarbonate and the samples were digested with 0.5 μg sequencing grade trypsin (Promega) over-night at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... Trypsin digested peptides were diluted to 200 μL with final concentration of 100 mM NH4HCO3 and 0.02% ProteaseMAX Surfactant prior to the addition of sequencing grade chymotrypsin (Promega) to a final 1:20 protease:protein ratio (w:w) ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were diluted to a final concentration of 2 M urea by addition of 100 mM NH4OAC (pH 6.8) and were digested overnight with sequencing-grade trypsin (Promega) at a 1:100 (enzyme:substrate ...
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bioRxiv - Cell Biology 2022Quote: ... Liquid was removed and gel pieces were dehydrated in 100% ACN at RT for 5 min at 2,000 rpm followed by rehydration in 100 mM NH4HCO3 containing 14 ng/μL trypsin (Promega) on ice for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... 40 μL of the cells’ supernatant containing secreted GLuc were mixed with Renilla luciferase Assay Reagent (100 μl) (Promega). GLuc activity was measured using SpectraMax i3 Multi-mode Detection Platform (Molecular Devices).
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bioRxiv - Cell Biology 2022Quote: ... Plates were then equilibrated to room temperature and lysed by the addition of Bright-Glo reagent (100 µL; Promega) to each well ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were diluted with 100 mM ammonium bicarbonate to a final urea concentration of 1.5 M and followed by digestion using trypsin (Promega) (37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng of starting RNA equivalent cDNA was subjected to PCR reaction with the GoTaq green master mix (Promega) and 30 amplification cycles (94°C for 30 s ...
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bioRxiv - Microbiology 2020Quote: ... alkylated with 100 mM iodoacetamide and proteolytically cleaved over night at 37°C using trypsin (Promega, Madison, WI, USA). Peptides were extracted ...
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bioRxiv - Neuroscience 2020Quote: A total of 100 μg protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation methods [19] ...
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bioRxiv - Genomics 2019Quote: ... samples were transferred to pre-cooled 1.5mL screw-tubes with 300 µL glass beads and supplemented with 100 units of RNAse inhibitor (RNAsin plus, Promega). Cells were lysed using a FastPrep-24 shaker (6.0m/s for 15 seconds ...
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bioRxiv - Plant Biology 2021Quote: ... Purified nucleus were recovered in 300 μl of Nuclei Lysis Buffer (50 mM Tris-HCl pH8; 0.1% SDS; 10 mM EDTA; 100 μM PMSF; RNasin PROMEGA) and 5 cycles (30 s ON – 30 s OFF ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of the total RNA was incubated in oxidization buffer (50 mM NaIO4 and 100 mM NaOAc pH 5.2) supplemented with RNasin (Promega) for 30 min at 25°C to oxidize the nonacylated tRNA 3’ end ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were precipitated overnight with 5 volumes of cold 0.1 M ammonium acetate in 100% methanol and digested with sequencing-grade trypsin (Promega) and each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: ... beads were resuspended in 100 μL of ABC buffer and digested by adding 0.2 μg of trypsine/LysC (Promega) in 100 µL of ABC buffer for 1 hour at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 4 pmol probe mix was combined with 0.5 or 100 ng of total RNA along with 4U µl RNasin Plus (Promega) and Ribohyb buffer ...
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bioRxiv - Genomics 2021Quote: ... ligation was performed at 15°C for 16 hours using 100 ng of the digested DNAs and 4.5 U of T4 DNA ligase (Promega) in a final volume of 100 μl ...
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bioRxiv - Immunology 2022Quote: ... Samples were diluted in 100 mM ammonium bicarbonate and incubated overnight with 0.5 μg/μl sequencing-grade trypsin (Promega) at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were diluted with 100 mM TEAB until containing 0.6 M urea and digested with sequencing grade modified trypsin (Promega) using a 1:150 enzyme-to-substrate ratio at 37°C for 16 hours ...
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bioRxiv - Microbiology 2022Quote: ... and the scrambled EK1 control were combined (~100 nM total final concentration) and translated in 50 µL reactions containing 12.5 µM supplemental tRNA (Promega), 50 U of Superase-In (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: ... The magnetic particles were then resuspended in 100 µL of 25 mM ammonium bicarbonate containing 0.5 µg of MS grade Trypsin/LysC (Promega) (enzyme/protein ratio of 1:20 (w/w) ...
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bioRxiv - Cell Biology 2023Quote: ... we co-transfected 100 ng of pGL4-Dusp13 or pGL4-Dusp27 with 50 ng of pRL-TK vectors (Promega) and varying amounts (0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Trypsin digested peptides were diluted to 200 μL with final concentration of 100 mM NH4HCO3 and 0.02% ProteaseMAX Surfactant prior to the addition of sequencing grade chymotrypsin (Promega) to a final 1:20 protease:protein ratio (w:w) ...
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bioRxiv - Cell Biology 2023Quote: ... Dynabeads-Ab-Ag complexes were resuspended in 100 µl NH4HCO3 and digested by adding 0.2 μg of trypsin-LysC (Promega) for 1 h at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... The size of the amplified PCR products was ascertained by utilizing a 100 bp GeneRulers™ DNA ladder (Promega). PCR products were sent to Genewiz/ Azenta company for DNA sequencing in both directions.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 20 min in 100 nM cell-permeable halotag conjugated to Janelia Fluor 549 dye (Promega) before washing twice with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Based on the migration of the amplified bands in relation to the industry-standard 100-bp DNA ladder (Promega), the size of the bands was further estimated (in nucleotide base pairs).
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bioRxiv - Molecular Biology 2023Quote: ... Beads were rinsed using 80% EtOH and proteins were digested with 100 µL of trypsin in 50 mM TEAB pH 7.55 (Promega, protein:enzyme ratio 1:50 ...
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bioRxiv - Microbiology 2023Quote: Approximately 100 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were then first dehydrated with 100% acetonitrile and subsequently rehydrated with sequence grade trypsin solution (0.6 µg, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pellet was resolved in 100 µL Cell Culture Lysis Reagent (Luciferase Assay System, E1500, Promega, Madison, WI, USA) on ice and cleared by centrifugation (8000 ×g ...
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bioRxiv - Molecular Biology 2023Quote: ... beads were resuspended in 100 μL of 25 mM NH4HCO3 and digested by adding 0.2 μg of trypsine/LysC (Promega) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed once in PBS and lysed by addition of 100 µL of 1X Passive Lysis Buffer (Promega) and incubated on a table-top rocker for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... beads were resuspended in 100 μl of 25mM ABC buffer and digested by adding 0.20 μg of trypsine/LysC (Promega) for 1 hour at 37 °C ...