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Citations for Promega :
1901 - 1950 of 4516 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... The sample was diluted with 100 mM tris to a final urea concentration of 1.5 M urea and digested with trypsin (Promega, Madison, WI) overnight at room temperature (1:50 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then co-transfected with 100 ng luciferase vector and 20 ng of pGL4-hRLuc-TK (Renilla, Promega, Madison, WI). 24 hours later ...
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bioRxiv - Microbiology 2020Quote: HPV infected or pGL3 transfected cells were washed once with PBS and lysed in 100 μL reporter lysis buffer (Promega E3971). Luciferase activity was measured on a DTX-800 multimode plate reader (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... The standard curve was generated by cloning a short 100-bp sequence of mitochondrial 16S rRNA gene into pGEM®-T Easy Vector (Promega). The 16S_rRNA gene sequence was amplified with Taq Polymerase (Supreme NZYtech II master mix ...
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bioRxiv - Plant Biology 2021Quote: ... and 100 μl sample volumes were assayed in triplicate for ATP content using the BacTiter-Glo bioluminescence kit (Promega, Mannheim, Germany). After 5 min incubation in the dark to allow for the decay of plate autofluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... Further 50ug in 100 µl protein sample was added into vials and for ATP measurement luminescence was measured through luminometer (Promega glowmax).
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of each supernatant were transferred into new 96 well plates and equal volume of BacTiter-Glo™ Reagent (Promega) was added per well ...
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bioRxiv - Microbiology 2022Quote: ... washing two times with 100 µl PBS and then lysed with 1x lysis buffer (made from 5x Promega Firefly Lysis Buffer). Plates were frozen for at least 24 hours and then thawed for 2 hours before analyzing with Promega Firefly Luciferase Kit on a luminometer.
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bioRxiv - Epidemiology 2019Quote: ... A band of about 350 base pairs (bp) in size was confirmed using a Ready-LoadTM 100 bp DNA ladder (Promega, USA). We submitted the positive PCR products for sequencing to Macrogen Inc (Seoul ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Infected SupT1 cells were cultured for another 48 h and directly lysed with 100 uL of Bright-Glo Luciferase Reagent (Promega, #E2610). Cell lysate was measured for luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were then reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40 °C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40 °C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 40 °C ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... MBP-LIC1 (50 pmol) and either BICD2N-Halo (50 pmol) or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281 ...
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bioRxiv - Immunology 2021Quote: ... 20 Nb L3 were collected with 100 μl of PBS and 100μl of the Celltiter-Glo Luminescent reagent (Promega, Madison, WI) was then added ...
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bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Loss of cell viability reflecting the efficiency of viral infection was measured 3 days after infection by adding 100 μL Cell Titer-Glo reagent to each well (CellTiterGlo Luminescent Cell Viability Assay, Promega #G7571), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40°C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40°C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 hours at 40°C ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 100 μl fresh TDB and HaloTag ligand (either in-house labelled Alexa647 ligand or Halo-Tetramethylrhodamine (TMR, Promega, USA) was added to a final concentration of 500 nM (Alexa647 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were lysed in 100 μl lysis buffer and subjected to promoter activity assay using the dual luciferase reporter assay system (Promega, USA). The luciferase enzymatic activity was measured using a PE Enspire Multilabel Reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: ... Northern blot was performed using poly(A)+ mRNA isolated from 100 µg of total CaSki RNA with PolyATtract mRNA Isolation Systems (Promega, #Z5310) or 10 µg of total CaSki RNA as described previously 8 ...
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bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
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bioRxiv - Bioengineering 2023Quote: ... 1 well-1 with 100 μL fresh cell culture media) and mixed with 100 μL reaction solution of CellTiter-Glo 3D cell viability assay kit (G9681; Promega, WI). The 96-well plate was gently rocked for 5 min and stabilized at room temperature for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pretreated with indicated antagonist for 1hr before treatment with 10 nM of PGF2a or 100 nM PGE2 overnight before glucose uptake was measured by Glucose Uptake-Glo™ Assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... HEK-293 PDE4A4 cell media is further supplemented with 500 μg/ml G418 (Promega). Culture conditions were 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and after adding 10 μL of 0.1 mg/mL GluC protease (Promega) incubated overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Digestion was performed by adding 10 μL of 0.2 mg/mL Trypsin Gold (Promega) and overnight incubation at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and dissociated in 50% Ham’s-12/DMEM medium containing 0.075 mg/mL liberase (Promega) for 30 minutes at RT ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... and rehydrated in 50 mM NH4HCO3 containing 10 μg / ml trypsin (sequence grade; Promega). After incubation overnight at 37 °C peptides were extracted and collected in a new tube ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were rehydrated in 60 μL of 20 μg/mL Trypsin (Promega) in 50 mM ammonium bicarbonate solution and incubated for 2h at 55°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... We rehydrated slices with 12.5 μg/ml modified porcine trypsin (Promega, Madison, WI, USA) in 50 mM AMBIC and digested overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... DNA samples were treated with a final concentration of 0.2mg/ml RNaseA (Promega #A7973) and 0.2mg/ml Proteinase K (NEB #P8107S ...
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bioRxiv - Immunology 2023Quote: ... droplets were resuspended in 30 ml of room temperature 6X SSC (Promega, Madison, WI) and 1 ml perfluorooctanol (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... grown up overnight in a 500 ml culture and maxiprepped (PureYield Maxiprep Kit, Promega). 400 μg of calmodulin and myosin-5 expression vectors (at a 1:6 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were dried and rehydrated with 12.5 ng/mL trypsin (Promega, Madison, WI) solution in 25 mM ammonium bicarbonate on ice for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Mice were intraperitoneally injected with 200 μl of 40 mg/ml D-luciferin (Promega) in PBS and anesthetized with isoflurane ...
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bioRxiv - Cell Biology 2020Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using random hexamers and MMLV reverse transcriptase (Promega). The cDNA was used as a template for quantitative PCR performed using Syber Green reagent (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
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bioRxiv - Molecular Biology 2021Quote: ... before cells were permeabilized using 0.2% Triton X-100 PBS for 10 min at room temperature, then blocked for 30 min (2% BSA / 0.5% fish skin gelatine / PBST.5, 2U/µL RNAsin Plus (Promega) at room temperature) ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 μl of f/2 and 10X concentrated substrate (10 mM firefly luciferin, 1,000X dilution of NanoLuc Dual-Glo reagent, Promega) were added to the respective wells.
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bioRxiv - Cancer Biology 2019Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Genetics 2021Quote: ... 2-3 μl of the clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... parental HeLa cells were transfected with 2 μg plasmid containing a GFP cassette and 4 μl FuGENE HD (Promega) in a six-well cluster ...
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bioRxiv - Bioengineering 2020Quote: ... the Nbs-virus mixture was removed from Vero E6 cells followed by 500 μL of DMEM with 2% FBS and 0.9% carboxymethyl cellulose (Promega) was overlaid ...
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bioRxiv - Genomics 2021Quote: ... Variant or variant haplotype fragments were amplified in African American heterozygous individuals carrying the variants of interest located in the middle of the fragments (Supplementary Table 2 and 3) and cloned into the enhancer reporter vector pGL4.24 (E8421, Promega). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was inserted into the multiple cloning site (MCS) of the psicheck-2 vector (supplied by Promega) downstream of the Renilla luciferase gene according to the manufacturer’s instructions ...