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Citations for Promega :
1551 - 1600 of 3625 citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 0.1 µl 1% digitonin (Promega,#G9441), 0.1 µl 10% Tween-20 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... anti-LgBiT (1:500, N7100, Promega), anti-MCPyV LT (1:500 ...
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Plant Biology 2021Quote: ... In vitro transcription and translation to radiolabel the proteins with [35S]-methionine was performed with the TnT® Quick Coupled Transcription/Translation System (Promega, Walldorf, Germany) in reticulocyte lysate ...
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bioRxiv - Microbiology 2021Quote: ... HepG2 or sg25 cells were transfected with a control plasmid or protein expression plasmids together with the luciferase reporter plasmids using Viafect TM Transfection Reagent (Promega, Madison, WI, USA) or Lipofectamine TM 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: The kinase activity of the FLT3-ITD protein was measured by using ADP-Glo™ Kinase Assay Kit (Promega, Madison, Wisconsin, the US). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Biochemistry 2023Quote: The cell free protein expression assays were performed by using the TnT® Quick Coupled Transcription/Translation System (Cat#L1171, Promega, Madison, USA), according to the manufacturer’s guideline with minor modification ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting proteins with carbamidomethyl groups on their cysteines were digested with 300 ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA) and injected on an Easy-nanoLC-1000 system coupled to a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: The caspase-1 activity assay was performed using the Caspase-Glo 1 inflammasome assay from Promega (G9951) as per the manufactures instructions ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 h at 37°C (sample to enzyme ratio 1:20) and then with GluC (Promega) (1:40 ratio ...
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bioRxiv - Biophysics 2020Quote: ... antibodies followed by a 1-hour incubation with the corresponding HRP-conjugated secondary antibodies (1:2000, Promega) at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Mcl-1 and Mcl-1-pomotor fragments were amplified and cloned into pMIR-REPORTM luciferase vector (Promega) to obtain HOXA-AS3-WT ...
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bioRxiv - Pathology 2024Quote: ... at a 1:50 wt:wt ratio alongside Lys-C at a 1:25 wt:wt ratio (Promega, VA1170) at 37 °C for 16 h ...
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bioRxiv - Immunology 2023Quote: Caspase-1 activity was measured in the supernatants using Caspase-Glo® 1 Inflammasome Assay (Promega G9951). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the gel lane was cut into 10 bands and the proteins were subjected to in-gel digestion with trypsin (Promega, Madison, WI, United States) as previously described (Leijon et al. ...
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bioRxiv - Plant Biology 2019Quote: ... Luminescence was measured for 1 sec every 1 min at room temperature using GloMax-Multi+Detection System (Promega). ROS production was expressed in relative luminescence units (RLU).
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of repair template was transfected into 200,000 cells using a 3:1 ratio Fugene6:DNA (Promega), after overnight transfection cells were grown in fresh medium for 6 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of pCMVi and 1 μg of pMDG (gifts from Greg Towers) using Fugene-HD (Promega, E2311). Virus was collected from 4x 10 cm plates 48 ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 mM NaCl, 1% NP-40, 0.5% SDS, 0.2 mM PMSF, 1 mM DTT, 10U RNase inhibitor from Promega, cOmplete Protease Inhibitor Cocktail from Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated for 1 hour at room temperature with an HRP-conjugated secondary antibody (1:1000, Promega). Protein expression was revealed using the Clarity kit (Biorad) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted 1:1 with TEAB buffer and digested by adding 0.1 µg of modified Trypsin (Promega) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega). The reaction was carried out at 42°C for 50 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega) in a total reaction volume of 25 μl ...
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bioRxiv - Genomics 2022Quote: ... After washing with TBST and incubation for 1 hour with anti-Rabbit IgG HRP Conjugate (Promega, 1:2,000) or anti-Mouse IgG HRP Conjugate (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... For immunostaining of the induced neurons following antibodies were used: anti-Tuj 1 (mouse; 1:2000, G7121, Promega), anti-MAP2 (chicken ...
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bioRxiv - Physiology 2022Quote: ... 1:1000 mouse anti-ATP1A1 followed by 1:2500 goat anti-mouse HRP-conjugated secondary (catalog #: W4021; Promega). p-ATP1A1Y260 and ATP1A1 bands were visualized with SuperSignal Pico utilizing the ChemiDoc.
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bioRxiv - Genetics 2020Quote: ... cells were washed with 1× phosphate-buffered saline (PBS) then lysed with 1× passive lysis buffer (E194A, Promega). Cell lysates were assayed in triplicate in a 96-well plate and luciferase activity was quantified using a GloMax microplate luminometer (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:10,000 for WB) or goat-anti-rabbit IgG (W4011, 1:10,000 for WB) were purchased from Promega. APC anti-human CD63 (353008 ...
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bioRxiv - Genomics 2022Quote: ... was resuspended in 1.5 mL wash buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) ...
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bioRxiv - Molecular Biology 2023Quote: ... molar equivalents of the appropriate TF constructs were transfected into cells alongside a 1:1 molar ratio of Luciferase (pGL4.23) to Renilla (pGL4.75, Promega) vectors ...
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bioRxiv - Plant Biology 2024Quote: ... 1 µL of extract was used as a DNA template for 1× Gotaq® Green Master Mix (Promega) 25 μL volume PCRs ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used included goat anti-rabbit IgG HRP-conjugated antibody (1:5,000, #7074S, CST) and goat/rabbit/donkey anti-mouse IgG HRP Conjugate (1:5,000, #W402B, Promega). The membranes for streptavidin blotting were blocked with 3% BSA ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... cDNA was diluted at 1/80 and qPCR were performed using 1 μl of diluted cDNA (GoTaq Promega) and run on QuantStudio 3 Real Time PCR system ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of random hexamer primers (Promega) and 1 μg of RNA in 16.125 μl ...
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bioRxiv - Cell Biology 2020Quote: ... Digested 1:50 v/v Trypsin (Promega) at 37°C o/n ...
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bioRxiv - Genetics 2021Quote: ... and α-βgal (1:100, Promega, Z3781). HRP-conjugated secondary antibodies in conjunction with the TSA system (Molecular Probes ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-Halotag (Promega, G9281, 1:500), rabbit anti-Sox2 (Abcam ...
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bioRxiv - Genomics 2021Quote: 1% of lambda phage gDNA (D1221, Promega) was spiked-into 300ng gDNA to use as an unmethylated internal control ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 unit/µl RNasin Plus (Promega, N2615), 10 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL GoScript reverse transcriptase (A5003; Promega), and 7 μL RNase-free double-distilled H2O (N2511 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-β-galactosidase (Promega, 1:2000); mouse anti-γ-tubulin (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... Rabbit monoclonal anti-pJNK (1:100; Promega) or rat anti-Dilp8 (1:50 ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-β-Galactosidase (1:1500; Promega), mouse anti-β-Galactosidase (40-1a ...
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bioRxiv - Microbiology 2020Quote: ... 1:1000 TuJ1 (monoclonal mouse, G712A, Promega); 1:250 NeuN (polyclonal chicken ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1% NLuc substrate (Nano-Glo, Promega) in the presence of release factors (4 nM eRF1 alone or with 4 nM eRF3a ...
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bioRxiv - Molecular Biology 2021Quote: ... trypsin-digested (1:75 w/w, Promega) and desalted (HLB μElution plate ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µg of sequencing grade Trypsin (Promega) was added ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µg of sequencing grade Trypsin (Promega) was added ...