Labshake search
Citations for Promega :
1351 - 1400 of 3625 citations for Recombinant HIV 1 GP120 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... import assays of the comparatively higher molecular weight ACE2 and S proteins (50 μL reactions) were both performed using the TNT® Coupled Transcription/ Translation system (Promega) for 90 min at 30°C as described by the manufacturer (∼50 ng/μL cDNA,1 μM Ipom-F or an equivalent volume of DMSO ...
-
bioRxiv - Plant Biology 2021Quote: ... In vitro kinase assays were performed by co-expressing the substrate together with a gate-keeper engineered phot1 (phot1GK) using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) in the presence of 10 μM FMN ...
-
bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... Further 50ug in 100 µl protein sample was added into vials and for ATP measurement luminescence was measured through luminometer (Promega glowmax).
-
bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
-
bioRxiv - Physiology 2022Quote: ... In-gel digestion of proteins was performed overnight at 37°C using trypsin (Promega Madison, WI, USA; 40 ng per band), and the resulting peptides were extracted twice (2 x 45 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... subjected to SP3 protein clean up as previously described (87, 88) and eluted in AmBic 50mM prior digestion with 300ng trypsin (Promega V5280). For the on-chromatin interactors ...
-
bioRxiv - Cell Biology 2022Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1 hour ...
-
bioRxiv - Biochemistry 2020Quote: ... mRNA for each chimeric Lep protein was produced from plasmids using the RiboMAX RNA production system in accordance with the manufacturer’s instructions (Promega, Madison, WI). Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega ...
-
bioRxiv - Synthetic Biology 2021Quote: All wheat germ cell-free expression used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Myc tagged full-length and truncated ARL13B fragments were generated by in vitro translation using TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning ...
-
bioRxiv - Plant Biology 2022Quote: ... PtaMYC2.2 and PtaJAM1.1 were cloned into pIXHALO (ABRC, Ohio State University) and expressed in vitro using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). We used empty vector pIXHALO as control ...
-
bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
-
bioRxiv - Molecular Biology 2023Quote: ... Then, the proteins were purified by acetone precipitation and digested with LysC (FUJIFILM Wako, Osaka, Japan) and trypsin (Promega, Madison, WI). The resulting peptides were desalted on InertSep RP-C18 columns (GL Sciences ...
-
bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... The quantity of the reporter protein was examined by measuring its enzymatic activity through the Renilla Luciferase Assay System (Promega, Germany), as per manufacturer’s instruction ...
-
bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
-
bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
-
bioRxiv - Biochemistry 2023Quote: ... Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega, cat. no. L4140) according to the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2023Quote: The FASP method[7] was used to digest urine protein with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA). One hundred micrograms of urine protein was added in the membrane of a 10KD ultrafiltration tube (Pall ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant (700 μL) was transferred to a sterile Eppendorf tube and mixed with 50 μL protein precipitation solution (Promega, USA) by inverting it ten times before centrifugation (10 min ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assessment of total cellular HiBiT-GCase protein abundance was performed using the Nano-Glo® HiBiT Lytic Detection System (Cat.#N3040, Promega), according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA (1 µg) was treated with RNase-free RQ1 DNase 1 (cat. # M6101, Promega) with a murine RNase inhibitor (cat ...
-
bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
-
bioRxiv - Neuroscience 2021Quote: ... and 1 μg of Lys-C and 1 μg of Glu-C (Promega, U.S.A.) were used for the sample for quantifying S182 phosphorylation.
-
bioRxiv - Microbiology 2022Quote: ... caspase-1 activity was measured using the Caspase-Glo 1 Inflammasome Assay kit (Promega) per manufacturer’s instructions.
-
bioRxiv - Genomics 2020Quote: ... pCGL-1-SNORD96A or empty pCGL-1 plasmid using Fugene (Promega, Madison, WI, USA). Cells were harvested 24- and 48-hours post-transfection for RNA isolation (TRIzol ...
-
bioRxiv - Molecular Biology 2022Quote: ... for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate) (Promega) overnight at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... The cell suspension was mixed 1:1 with ONE-GloTM Luciferse Assay Buffer (Promega), and the luminescence was measured with the Synergy HTX Multi-Mode Reader ...
-
bioRxiv - Molecular Biology 2023Quote: The PD-1/PD-L1 immune checkpoint bioassay (PD-1/PD-L1 Bioassay, Promega) was performed according to the manufacturer’s manual ...
-
bioRxiv - Microbiology 2023Quote: ... 200 µl elution buffer (1 M urea, 100 mM NH4CO3, 1 µg Trypsin (Promega)) was added to the beads and incubated at 1000 rpm on a thermomixer at 27°C for 45min ...
-
bioRxiv - Cell Biology 2023Quote: ... Subsequent digestion was performed with Trypsin/LysC solution [100ng/μl 1:1 Trypsin (Promega):LysC (FujiFilm ...
-
bioRxiv - Microbiology 2024Quote: ... Briefly, 100 µl elution buffer 1 (100 mM ammonium bicarbonate, 1 µg trypsin (Promega)) was added to each sample ...
-
bioRxiv - Developmental Biology 2021Quote: α-βgal (1:100, Promega, Z3781) was used to visualize rP298.lacZ ...
-
bioRxiv - Systems Biology 2022Quote: ... 0.5μl 1% Digitonin (Promega G9441), 9.5μl H2O ...
-
bioRxiv - Neuroscience 2019Quote: ... and RNasin (Promega, 1:1000) to obtain a final volume of 1.5 ml ...
-
bioRxiv - Cell Biology 2019Quote: ... and 1 μl dNTP (Promega). Minus RT blanks were prepared from 10 μl of the same RNA sample under the same conditions without the addition of reverse transcriptase ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 µg trypsin (V511A, Promega) was added to both samples (−/+ Triton ...
-
bioRxiv - Neuroscience 2020Quote: ... fLuc (1:1000, G7451, Promega), eIF2D (1:2000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Tuj1 (1:1000, Promega G7121), H4K8ac (1:1000 ...
-
bioRxiv - Zoology 2021Quote: ... 1 μl 10mM dNTPs (Promega), 2.5 μl of each primer at 10 μM ...
-
bioRxiv - Biochemistry 2020Quote: ... and 1% nanoluciferase substrate (Promega), were incubated in 384-well plate (Nunc ...
-
bioRxiv - Systems Biology 2020Quote: ... 1 mM UDP-Gal (Promega) for B3GALT2 ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.5 µl 1% Digitonin (Promega), 0.5 µl 10% Tween-20 (Sigma) ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.5 µl 1% Digitonin (Promega) (resuspension buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 U/μl RNasin (Promega) and 1 mM ATP at 16°C overnight ...
-
bioRxiv - Cell Biology 2021Quote: ... Furimazine (1:20 dilution, Promega) was then added and the plate promptly transferred to a Flexstation 3 plate reader at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate (1:10,000) (Promega). The experiments were repeated three times independently with similar results.
-
bioRxiv - Neuroscience 2021Quote: ... 1 μL of RNaseOUT (Promega) and 1 μL of reverse transcriptase (M-MLV RT ...