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Citations for Promega :
1501 - 1550 of 4405 citations for Mouse Vasoactive intestinal polypeptide receptor 2 VIPR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Expression vectors encoding Foxp3 and Ikzf1 and/or Ikzf1 mutants were transfected into HEK293T cells (2 × 105) with FuGENE HD (Promega). 48h after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... cells were starved by 50 μL Hank’s balanced salt solution for 30 min and then incubated in 50 μL CO2-independent media containing 2% GloSensor cAMP Reagent (Promega) for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Microbiology 2023Quote: ... Specific adapters 1 and 2 described in Table B were self-hybridized and then ligated to the ‘A’ tail ends by T4 ligase (Promega). After purification with AMPure XP purification kit (Agencourt) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were diluted with 50 mM NH4HCO3 to a final concentration of 2 M urea and digested with trypsin (Promega), at an enzyme-to-substrate ratio of 1:20 ...
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bioRxiv - Neuroscience 2023Quote: ... luminescence photons were collected by accumulating the image for 60 s in the presence of 2 mM D-luciferin (Promega), and the luminescence signal was measured from the same varicosities as the corresponding fluorescence image ...
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bioRxiv - Biochemistry 2023Quote: ... then washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg/μl sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off with spin columns (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... First-strand complementary DNA (cDNA) synthesis was performed using 2 μg of total RNA with M-MLV reverse transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2023Quote: The activity of EBV miRs-BART 7-3p and 9-3p was evaluated in Akata-EBV/Cas9 cells by luciferase reporter gene assay with constructs based on the psiCheck-2 backbone (Promega). 3’-UTR sequences with miRNA-binding sites (Supplementary Material ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNAs were detected with a YFP long probe (primers listed in Supplemental Table 2) labeled with 32P-dCTP prepared according to the manual of the Prime-a-Gene Labeling System (Promega). The blot was hybridized overnight at 42 °C with the probe before being washed with 2xSSC ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma], 20 U ml-1 RNase inhibitor [Promega] ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.
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bioRxiv - Evolutionary Biology 2023Quote: ... reverse transcription was performed using Promega’s Go-Taq 2-Step system with oligo(dT) and randomized primers as per manufacturer’s instructions (Promega, Madison, WI).
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 4% FBS and 100 µL were seeded per well in 96-well plates at a density of 2×105 cells/mL in the presence or absence of 0.1 mM HaloTag NanoBRET™ ligand (Promega). The following morning ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours followed by another overnight digestion with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... Intracellular fluorescence was monitored after 2 and 3 h of nanoalgosome incubation by spectrofluorimetric analysis using a microplate reader (Glomax, Promega). As vehicle-control ...
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bioRxiv - Microbiology 2024Quote: ... Tat and Rev and plasmid encoding spike of SARS-CoV-2 variants Delta or Omicron were transfected in HEK 293T cells using Fugene6 reagent (Cat. No. E2691, Promega) following manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Biochemistry 2023Quote: ... pLH3/DTX3L or pLH3/DTX3LΔN) and accessory plasmids pMD2g and psPAX2 (ratio 2:1:1) using ViaFect transfection reagent (Promega E498A). After cell incubation at 37°C for ∼16 h ...
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bioRxiv - Systems Biology 2023Quote: ... The panel of ssDNA-labelled antibodies (250 ng/ml for each antibody) was mixed with 2 U/µl RNAsin Plus (Promega) and 0.1% Triton X-100 in PBS:PFBB (1:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR fragment was directionally cloned downstream of the Renilla luciferase ORF in the psiCHECK-2 vector (Promega, Madison, US) using the Quick Ligase kit (M2200 ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Pathology 2024Quote: ... The samples were diluted with 50 mM TEAB buffer to a final Urea concentration of 2 M before adding trypsin (Promega) in an enzyme:substrate ratio of 1:75 and incubating at 25 °C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... 48 hours later the media was removed and replaced with 25 µL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 µg/mL to a final concentration of 0.014 µg/mL and 25 µL of antibody dilution was added to the plate ...
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bioRxiv - Neuroscience 2024Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21ºC overnight ...
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bioRxiv - Cell Biology 2023Quote: ... dilute with 4 volumes of 20 mM Tris-HCl pH8 / 2 mM CaCl2 before overnight digestion at room temperature with 100 ng sequencing grade trypsin (Promega). Samples were centrifuged ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: All Plasmids were purified using the Midiprep kit (QIAGEN or Promega)
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bioRxiv - Cancer Biology 2021Quote: Dual-luciferase report assay was determined with the kit (Promega, E1910) following manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... The luciferase activity were measured using a luciferase assay kit (Promega) and subtracted with the background level obtained from co-cultures in the absence of mAb.
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bioRxiv - Cell Biology 2020Quote: ... used for the Luciferase reporter assay (Steady Glow Luciferase Kit, Promega) and quantified as Relative Light Unit (RLU ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts were analyzed using the Dual-Luciferase Reporter Assay Kit (Promega). The ratio of Luciferase/Renilla was calculated and normalized to experimental control (promoter in absence of experimental plasmid).
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bioRxiv - Cell Biology 2020Quote: Luciferase assays were performed with the dual luciferase assay kit (Promega). Cells were lysed using passive lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Luciferase expression levels were analyzed using the Dual Luciferase kit (Promega) in a DTX880 Multimode detector (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with the M-MLV reverse-transcriptase kit (Promega) and diluted 1 in 5 before further application ...
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bioRxiv - Genetics 2021Quote: ... PCR were performed with the kit GoTaq® Flexi (Promega, USA) using 20 ng DNA ...
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bioRxiv - Neuroscience 2020Quote: ... and transcribing using the T7 RiboMAX RNA Production kit (Promega-P1320). Cas9 was transcribed from the plasmid pCS2-Cas9 (Addgene ...
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bioRxiv - Genomics 2021Quote: ... and Maxwell® RSC ccfDNA Plasma Kit (Promega, Leiden, the Netherlands). DNA was eluted in elution buffers provided by the kits used (QIAamp kit ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using the Maxwell Cell tissue kit (Promega). The primers ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: Cell viability was assayed using the CellTiter-FluorTM kit (Promega, G6081) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity was measured by Luciferase Assay kit (Promega, WI, USA). The 50% neutralization titer (NT50 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The CellTiter-Glo® Luminescent Cell Viability Assay Kit (Promega, # G7570) was used as recommended in the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... with an adenosine triphosphate (ATP) content assay kit (catalog 6016949, Promega).
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was reverse-transcribed using GoScript Reverse Transcriptase Kit (Promega). Second ...
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bioRxiv - Cell Biology 2022Quote: ... A549 cells were solubilized using the Dual-Luciferase Assay Kit (Promega), and luciferase activity was quantified using a microplate dual-injector luminometer (Veritas ...
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bioRxiv - Immunology 2022Quote: ... cDNA was then synthesized using the reverse transcription kit from Promega. Transcriptional levels of PD-L1 (Forward ...