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Citations for Promega :
1451 - 1500 of 5340 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... RPS18 and UBE2D2 using the corresponding PCR master mix (Promega GmbH, Germany) on a CFX96 Detection System (Bio-Rad Laboratories GmbH ...
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR reactions were performed using GoTaq Green Master Mix (Promega, M7123) with 0.25 ng total cDNA per reaction ...
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bioRxiv - Genetics 2023Quote: ... PCR products were gel-purified and cloned into pGEM-T Easy (Promega) for Sanger sequencing ...
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bioRxiv - Immunology 2023Quote: ... Quantitative PCRs (qPCRs) were performed with GoTaq ® qPCR Master mix (Promega) following the standard cycling conditions suggested by the manufacturer in a StepOnePlus real time PCR system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... was added and the mixture was incubated for 30 min at room temperature followed by 30 min incubation with detection reagent (Promega MTase-Glo™ Methyltransferase Assay). Luminescence was detected using a SpectraMax iD5 (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... After washing with 150 μL of binding buffer four times the samples were subjected to proteolytic digestion using 1.2 μg trypsin (sequencing grade, Promega) for 2h at 47°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was measured at the indicated time points by the addition of CellTiter-Glo 2.0 reagent (Promega #G9242) and luminescence was measured (POLARstar Optima plate reader ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were allowed to grow for two doubling times and proliferation was measured by CellTiter-Glo® (Promega G7571) or CyQuant (Invitrogen C3511 ...
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bioRxiv - Genomics 2021Quote: ... Cell viability was measured at indicated times post-electroporation using the CellTiter-Glo luminescent cell viability assay (Promega G7570).
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bioRxiv - Cancer Biology 2019Quote: ... cells were seeded in 5.000 for well in a 96 well plate and treated with the indicated compound and time points and cell viability was evaluated by Cell Titer-Glo 2.0 (Promega) according to manufacturer instruction ...
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bioRxiv - Systems Biology 2023Quote: ... Cell viability was measured at indicated times post-electroporation using the CellTiter-Glo luminescent cell viability assay (Promega G7570).
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bioRxiv - Cell Biology 2022Quote: ... Three hundred micrograms of each sample were diluted 10 times with 20 mM Tris and digested with 1.5 μl of Mass Spectrometry Grade Trypsin Gold (#V5280, Promega) at 37 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... Time-dependent bioluminescence was measured after glutamine addition using Nano-Glo® Dual-Luciferase® reporter assay system (Promega). Cell culture plates were equilibrated at 22 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell number was measured at the indicated time intervals with CellTiter-Glo® Luminescent Cell Viability Assay (Promega #G7571) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Beads were washed 6 times with 50 mM ammonium bicarbonate and then treated with TPCK-treated modified trypsin (Promega) for 16 hours at 37°C on an end-over-end rotator ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 5 times with wash buffer (PBS with 1% BSA (Capricorn Scientific) and 0.05% Tween-20 (Promega)) ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system.
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Cell Biology 2019Quote: ... The obtained PCR-product was cloned into the pGEM-T Easy vector (Promega). Several plasmid clones were Sanger sequenced and plasmid clones with 26 bp XBP1 intron were found ...
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bioRxiv - Genetics 2021Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega), cloned into the pGEM-T Easy vector (Promega) ...
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Relationship between True Digestibility of dietary Phosphorus and Gastrointestinal Bacteria of GoatsbioRxiv - Microbiology 2019Quote: ... The amplicons were purified with a PCR Clean-Up system (Promega, Madison, USA) by a purification kit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... the PCR products were subcloned into pGEM-T Easy (Promega, Madison, WI USA) for sequencing analyses using a Genetic Analyzer (ABI 3130 ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR-based genotyping (GoTaq Green MasterMix, Promega, and C1000 Touch Cycler, Bio-rad) was used to detect the genes ...
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bioRxiv - Molecular Biology 2020Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) or MinElute® PCR Purification Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaned with Wizard SV Gel and PCR Clean-Up system (Promega, Madison, WI) and cloned into One Shot TOP10 cells with TOPO-TA cloning kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100ng of gDNA was amplified by PCR with GoTaq DNA polymerase (Promega). Sequences and specifications of the primers are shown in Table S1.
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bioRxiv - Molecular Biology 2021Quote: ... and 100ng of gDNA was amplified by PCR with GoTaq DNA polymerase (Promega). Sequences and specifications of the primers are shown in Table S1.
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... The PCR product was cloned into the pGL3-promoter luciferase-based plasmid (Promega) at the cloning site between KpnI and XhoI ...
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bioRxiv - Evolutionary Biology 2019Quote: ... These regions were PCR-amplified with GO taq Colorless Master Mix by Promega using primers designed for regions conserved across the six mitogenomes assembled in this study (data in S1 Text) ...
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bioRxiv - Plant Biology 2019Quote: ... 12.50 μl of 2× PCR Green master mix (GoTaq DNA polymerase; Promega, USA) and 8.50 μl nuclease free water ...
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bioRxiv - Neuroscience 2019Quote: ... were PCR-amplified and subcloned into the pGEM-Teasy vector (Promega, Madison, WI). The resultant constructs were used to generate digoxigenin (DIG)-labeled cRNA probes for npba ...
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bioRxiv - Bioengineering 2021Quote: ... with PCR and cloned into the pCDH-CMV-MCS-EF1-Puro vector (Promega). For the reporters containing disease-relevant mutations ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR fragments were cloned into the pGEM-T Easy vector (Promega, USA) and the amplified sequences were confirmed by direct sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... and the PCR product was subcloned into pGEM®-T Easy Vector (Promega). The plasmid was purified with Plasmid Midi Kit (QIAGEN ...
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bioRxiv - Neuroscience 2020Quote: ... and these PCR products were used as templates for in vitro transcription (Promega). Antisense riboprobes were labeled with digoxygenin-dUTP or fluorescein-dUTP (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: SqRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, TM318), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... the individual PCR products were directly ligated into the pGEM-T Easy (Promega) vector yielding level I vectors LI A-B pNPH3 and LI C-D NPH3 ...
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bioRxiv - Cell Biology 2021Quote: ... Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega) spiked with α-32P-deoxycytidine triphosphate (dCTP) ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR analysis was performed using GoTaq® qPCR Master Mix (Promega, WI) with Light Cycler 96 (Roche Life Science ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of 2× SYBR Green PCR Master Mix (Promega, Madison, WI, USA) and sterile milliQ water to a final volume of 15 μl per tube ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting PCR products were cloned into the pGEM-T easy vector (Promega) and transformed into E ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR product was first cloned in the pGEM T Easy vector (Promega) and sequenced ...
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bioRxiv - Molecular Biology 2022Quote: ... Screening of the colonies was performed by colony PCR using GoTaq Polymerase (Promega). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed with GoTaq Green Master Mix (Promega Corp., Madison, WI, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Relative quantitative PCR analysis was performed using the GoTaq qPCR SYBR mastermix (Promega) on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Developmental Biology 2022Quote: ... and purified using a Wizard SV Gel and PCR Clean-up System (Promega). mRNA was generated from linearized plasmid using an SP6 mMessage mMachine Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR validation system was set up using GoTaq Green Master Mix (Promega) with the following components ...
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bioRxiv - Neuroscience 2021Quote: PCR products were subcloned into pGEM-T easy vectors (Promega, Madison, WI, USA). The resultant plasmids were sequenced commercially (Fasmac ...