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Citations for Promega :
1451 - 1500 of 2468 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the sense robo2 probe was synthesized from pBlueScript-robo2 linearized with XhoI using T7 RNA polymerase (Promega). Probes were hydrolyzed in 0.6M sodium carbonate and 0.4M sodium bicarbonate at 60°C for 11min to yield 300-500bp fragments ...
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bioRxiv - Neuroscience 2020Quote: ... The antisense robo2 probe was synthesized from pBlueScript-robo2 linearized with EcoRI using T3 RNA polymerase (Promega); the sense robo2 probe was synthesized from pBlueScript-robo2 linearized with XhoI using T7 RNA polymerase (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA was isolated from bacterial cells using commercial Wizard Plus SV Minipreps DNA Purification kit (Promega) or the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from leaves using the Maxwell® 16 Instrument LEV simplyRNA Purification Kits (Promega). All reverse transcription reactions were carried out using random primers and the High-capacity cDNA reverse transcription kit (Applied Biosystems®) ...
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bioRxiv - Microbiology 2020Quote: Single cell suspensions obtained from tissues or infected cells were lysed in 1X Passive Lysis Buffer (Promega) for 5 min at 37°C and lysates were added to white-bottom 96-well flat-bottom plates (Costar ...
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bioRxiv - Microbiology 2021Quote: ... The firefly luciferase signal from infected cells was determined using the Bright-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Assay conditions were adopted from previously described methods incorporating the P450-Glo™ Assay (Promega, Madison, WI).23,100-104 Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... to remove genomic DNA from sample and protected against RNA degradation using RNasin® Ribonuclease Inhibitor (Promega). RNA concentration and quality were then measured using a NanoDrop™ 2000 spectrophotometer.
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bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial DNA was extracted from 1 ml of culture using the Wizard genomic DNA purification kit (Promega). Evolved population DNAs were sequenced at the GeT-PlaGe core facility (https://get.genotoul.fr/) ...
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bioRxiv - Molecular Biology 2022Quote: ... the HIV 5’ LTR from the pNL4-3 isolate (Genbank AF324493) was cloned into pGL3-Basic (Promega) via Gibson assembly (NEB 2X HiFi ...
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bioRxiv - Molecular Biology 2020Quote: ... Activities from firefly and Renilla luciferases were assayed with the Dual-Luciferase Reporter Assay system (Promega Corp.) on a Mithras LB940 (Berthold Technologies Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA from each sample was treated with RQ1 RNase-Free DNase (Promega Co.) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 300 ng from each RNA extraction was treated with DNAse (Promega, Madison, WI, USA) and reverse transcribed with Superscript II reverse transcriptase (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and were reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: Genomic deoxyribonucleic acid (gDNA) was isolated from each mutant using the Wizard® gDNA purification kit (Promega). Illumina NextSeq was then performed by the Genomic Services Facility at Indiana University Center for Genomics and Bioinformatics ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from 2µg of DNA free RNA using MMLV reverse transcriptase and random primers (Promega). Quantification of the cDNA was carried out in the Rotor gene 3000A machine (Corbett ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the dCK gene was PCR-amplified from pDNR-Dual_dCK (DNAsu HsCD00000962) using Pfu DNA polymerase (Promega, Germany) and gene-specific primers (Eurofins Genomics ...
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bioRxiv - Biochemistry 2021Quote: Histones extracted from HeLa cells were dissolved in 50 mM NH4HCO3 buffer and digested with trypsin (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... blaTEM and blaOXA were cloned from strain 48 into the cloning vector pGEM-T easy (Promega, UK) using the HiFi assembly kit (New England ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from ear homogenates and reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega). Real-time PCR was performed on an ABI PRISM 7500 sequence detection system (Applied Biosystems ...
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Spatial control of gene expression in flies using bacterially derived binary transactivation systemsbioRxiv - Genetics 2020Quote: ... the Passive Lysis Buffer 5X from the Dual-Luciferase® Reporter Assay System kit (Promega, Cat.# E1910) was diluted in distilled H2O at 1:5 to make a 1x lysis buffer ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA was isolated from EDTA blood samples using the Maxwell RSC Whole Blood DNA kit (Promega).
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bioRxiv - Genetics 2020Quote: ... purified from agarose gels using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA) and sent for sequencing (to GATC Services-Eurofin Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... ImProm-II™ Reverse Transcription System and GoTaq® DNA polymerase were purchased from Promega (Madison, WI). The RNA Clean & Concentrator™-5 was purchased from Zymo Research (Irvine ...
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bioRxiv - Physiology 2021Quote: ... Complementary DNA (cDNA) was synthesized from total RNA (300 ng) using M-MLV Reverse Transcriptase (Promega, #M1701) as per supplier’s protocol (1hr at 37°C followed by 10 min at 70°C) ...
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bioRxiv - Cell Biology 2021Quote: ... RNA from laser-microdissected areas were extracted with a ReliaPrep RNA cell Miniprep kit (Promega, Madison, USA) according to the manufacturer’s protocol and quantified/qualified with Nanodrop and Agilent 2100 Bioanalyzer apparatus (RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was prepared from bacterial cultures grown at 37 °C using PureYield™ Plasmid Midiprep System (Promega) according to manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Total genomic DNA was extracted from the pelleted cells using Wizard DNA purification kit (Promega, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega, UK, Cat. A5003). Primers were designed using NCBI BLAST primer design or ...
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bioRxiv - Microbiology 2020Quote: ... The radiolabeled probe was random primed from this DNA amplicon using Prime-a-Gene Labelling System (Promega) and 32P-dCTP (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction from harvested tissues was carried out using a Maxwell® RSC simplyRNA Tissue Kit (Promega) and cDNA generated using ABI High Capacity cDNA Reverse Transcription Kits (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was extracted from the different clones using the Wizard SV Genomic DNA Purification System (Promega). The DAP5 locus was PCR amplified and Sanger sequencing of the targeted genomic regions indicated two frameshift mutations in exon 10 (172 bp deletion in exon/intron 10 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was produced from 1 μg of RNA sample using M-MLV reverse transcriptase (Promega; M-1302). Real time qPCR was preformed using a QuantiNova SYBR Green kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... isotypes from patients were pooled at equal concentrations and concentrated using Wizard PCR Clean-up kits (Promega) according to manufacturer’s instructions with 30 μl of elute ...
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bioRxiv - Genomics 2021Quote: ... Mini-promoter and mCherry (miniP-mCherry) fragments were obtained from modified pGL4.23 luciferase reporter vector (Promega, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... after isolating DNA from mouse tissue samples using a tissue DNA extraction kit (Maxwell® 16, Promega), the purified DNA was incubated and hydrolyzed enzymatically (EpiQuik ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells from the host shutoff assays above were harvested and lysed in passive lysis buffer (Promega) for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... minigenome replication was evaluated by measuring the luminescence of NanoLuc from the minigenome using Nano-Glo (Promega).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pTargeT mammalian expression vector and P450-Glo CYP3A4 Assay (Luciferin IPA) were obtained from Promega (Madison, WI). RNAiso and random hexamers were from Takara (Shiga ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated from the DS-12 isolate using the Wizard gDNA Purification Kit (Promega A1125) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were transfected with the respective constructs using Fugene® HD from Promega (Madison, Wisconsin, USA) or Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Physiology 2023Quote: ... Thirty μg of total protein extract was isolated from the VSMCs using 1X passive lysis buffer (Promega) and was mixed with radio-labeled oligo for binding ...
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bioRxiv - Genetics 2023Quote: ... from HEK293A cells transfected with CROPseq-EFS-SpCas9-P2A-EGFP DNMT3B sgRNA plasmid using Fugene HD (Promega). PCR fragment for SURVEYOR assay was amplified using Q5 high-fidelity polymerase using the primers F ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA was then extracted from each sample using the Wizard genomic DNA purification kit (Promega, A1120). Genomic sites of interest were amplified from purified genomic DNA and amplicons were sequenced on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Genomics 2023Quote: Genomic DNA was extracted from 16-day-old seedlings with the Wizard Genomic DNA Purification Kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Tol2-mpx:mCherry-2A-rac2 [43] (a gift from Anna Huttenlocher) was cut with NheI and SalI (Promega) to remove the mpx:mCherry-2A-rac2 insert ...
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bioRxiv - Molecular Biology 2022Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a RT-PCR kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA from leaves was extracted using a Maxwell 16 LEV Plant RNA Kit (Promega, WI, USA). The amount of RNA was determined using Quant-iT RNA Assay Kit ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots using an RNA Purification kit as described by the manufacturer (Promega), followed by DNase I treatment ...