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Citations for Promega :
1451 - 1500 of 4408 citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cell were lysed according to the ONE-Glo™ luciferase assay system (Promega, Madison, US) and light emission was recorded using a Berthold® TriStar LB941 luminometer ...
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bioRxiv - Cell Biology 2020Quote: ... the MTS reagent (20μl, CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, USA) was added to wells for the D0 timepoint and incubated for 2.25 h in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... Whereas one aliquot of cells was lysed immediately with luciferase lysis buffer (Promega, Madison, WI), two additional similar aliquots (second and third ...
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bioRxiv - Cancer Biology 2020Quote: Cell viability was determined by MTS (CellTiter 96 Aqueous One Solution Reagent, Promega, Wisconsin, USA) as previously described[65] ...
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bioRxiv - Genomics 2021Quote: ... A Quantus fluorometer and a QuantiFluor™ ONE dsDNA System (Promega Corporation, Madison, WI, USA) were used to estimate the DNA concentration.
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bioRxiv - Developmental Biology 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega). Quantitative RT-PCR was performed in triplicate with custom-designed oligos using the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... luciferase-based reporter assays were performed using the ONE-GloTM Luciferase Assay System kit (Promega). COS7 cells were transfected with bacterial plasmid constructs expressing luciferase under the control of the ligand-binding domain for LXRα or LXRβ ...
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bioRxiv - Neuroscience 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega), thereafter qRT-PCR was performed in triplicate with custom designed oligos (Table S3 ...
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bioRxiv - Molecular Biology 2022Quote: One-hundred μl protein expression reaction of TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIXHALO-bZIP plasmids and 1500 ng pIXSBP-bZIP plasmids were incubated for 3 h at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... the infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130), a Bright-Glo luciferase assay system (Promega ...
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bioRxiv - Microbiology 2022Quote: ... the infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130), a Bright-Glo luciferase assay system (Promega ...
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bioRxiv - Cancer Biology 2023Quote: Luciferase activity was determined using the One Glo Luciferase Assay System (Promega, Walldorf, Germany, #E6110). For each replicate ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation assay was measured with CellTiter 96 AQueous One Solution (MTS, Promega, #G3580). The assay was performed according to the methods described in the manufacturer’s manual ...
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bioRxiv - Biochemistry 2023Quote: ... the transfection potency of mLuc was measured using the One-Glo Luciferase Assay System (Promega), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with CellTiter 96® AQueous One Solution Cell Proliferation Assay reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Viable cells were detected after the addition of CellTiter 96 Aqueous One Solution reagent (Promega) by measurement of absorbance at 490 nm ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130), a Bright-Glo luciferase assay system (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... Lactate dehydrogenase release was detected using the CytoTox-ONE Homogenous Membrane Integrity Assay Kit (Promega), as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured using CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 µL of lysate was then mixed with 25 µL of ONE-Glo (Promega # E6120) or 25 µL of prepared Nano-Glo Luciferase Assay System (Promega # N1120 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, G3582).
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bioRxiv - Synthetic Biology 2024Quote: Signal of the Fluc reporter was quantified with the ONE-Glo Luciferase Assay System (Promega) and Nluc reporter was quantified with Nano-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... at one week and at two weeks and measured by CellTiter-Glo (Promega #PR-G7573) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by measuring luciferase activities using ONE-Glo™ EX Luciferase Assay System (Promega E8110) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cell proliferation was measured using CellTiter 96 AQueous One Solution Cell Proliferation Assay (G3582, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and one day later the cells were transfected with pISRE-luc and pTK-RL (Promega) using TransIT®-LT1 Transfection Reagent ...
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bioRxiv - Molecular Biology 2024Quote: MTS assays were undertaken using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega) or Abcam MTS assay kit (ab197010) ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability and proliferation was assayed using CellTiter 96 Aqueous One Solution Reagent (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... 4 μg of RNase A (Promega), and 40 units of RNase ONE (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 4 µl RT buffer 5x (Promega), 2.5 μl dNTPs 2.5 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Microbiology 2020Quote: ... The protein mixture was diluted four times and digested with LysC at 1:50(w/w) for four hours and then with Trypsin at 1:40 (w/w) (Promega, http://www.promega.com/) overnight.
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bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were digested with 1 μg of lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg of trypsin (Promega, Tokyo, Japan) overnight at 37 °C in a shaking incubator ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Microbiology 2021Quote: ... Standard RNA was synthesized from a partial region of the GPC gene using the forward (5’-TAATACGACTCACTATAGGGCCAACCTTTTTGCAGGAGGC-3’) and reverse (5’-AGCTTCTTCTGTGCAGGATCTTCCTGCAAGCGCTAGGAAT-3’) primers and the T7 RNA polymerase (Promega), as previously described (Pemba et al. ...
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bioRxiv - Microbiology 2022Quote: ... The region of recombination was amplified using primers PV3-F2 (5′-CTCCAAAGTCCGCATTTACA-3′) and PV1-R2 (5′-ATCAGGTTGGTTGCTACA-3′) and Taq polymerase (Promega) with an initial denaturing at 95°C for 2 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The following day activity of caspases 3 and 7 was measured using the Caspase-Glo® 3/7 Assay (Promega), according to the manufacturer’s instruction.
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bioRxiv - Cell Biology 2019Quote: ... The full-length coding sequence of murine Tmem98 was amplified from a mix of murine embryonic and murine keratinocyte cDNA using forward 5’-AAAAAGCTTGCCATGGAGACTGTGGTGATCGTC-3’ and reverse 5’-TTTTGAATTCTTAAATGGCCGACTGTTCCTGCAGGAAGC-3’ primers and cloned into pSP72 (Promega) as a HindIII/EcoRI fragment ...
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bioRxiv - Microbiology 2021Quote: ... Apoptosis was quantified by measuring caspase 3/7 activation of a luminescent signal using Caspase-Glo 3/7 assay (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... The resulting 3’UTR-Wild and 3’UTR-Mutant genes were inserted into the XbaI restriction site of pGL3-control vector (Promega) expressing firefly luciferase ...