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Citations for Promega :
101 - 150 of 2997 citations for Vitamin K epoxide reductase complex subunit 1 VKORC1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Cleaved MSP protein was subsequently biotinylated using EZ-Link Maleimide-PEG11-Biotin (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... samples were blocked in 20% normal goat serum (NGS) for 1 h and incubated with the HaloTag® Biotin Ligand (Promega, 200-fold dilution) in PBS supplemented with 5% NGS overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Genetics 2024Quote: ... in ultrapure water with 2.5 µL proteinase K (20 mg/mL; Promega, Southampton, UK). The samples were incubated for a minimum of 60 minutes at 56 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for 24 h with a complex of DNA and FuGENE® HD Transfection Reagent (#E2311; Promega) at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070 ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA encoding for SthCsm-complex and guide RNA was mixed with 1.5 μL of FuGENE HD reagent (Promega), incubated for 15 min ...
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bioRxiv - Biophysics 2022Quote: ... The purified Kif5B protein was incubated with 10 µM HaloTag PEG-Biotin ligand (Promega) for 30 min on ice to produce a final construct of Kif5B homodimer with two C-terminal biotin tags ...
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bioRxiv - Microbiology 2020Quote: ... The peptides were spotted onto streptavidin-coated filter sheets (Promega SAM2 biotin capture membrane) and visualized by phosphorimaging on Typhoon FLA 7000 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subjected to a pulse with 5 µM biotin-HaloTag ligand (G828A, Promega) diluted in complete medium for 3 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HaloTag Alexa Fluor 488 ligand (G1001) and HaloTag Biotin ligand (G8281) were from Promega, USA ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000, Promega). Chemiluminiscent signals were detected with an ECL Prime Western blotting detection kit (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were stopped by adding a buffer containing 50 μg/mL of proteinase K (Promega) in a buffer of 3 mM CaCl2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Systems Biology 2023Quote: Methyltransferase activity of the NSP10-NSP16 complex and inhibitor screening was performed using the MTase-Glo assay (Cat. No. V7601, Promega) (Hsiao et al ...
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bioRxiv - Microbiology 2023Quote: The glycosyltransferase activity of the CslA-GlxA complex was quantitatively measured by using the UDP-GloTM glycosyltransferase assay kit (Promega). This assay detects the content of free UDP ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Secondary antibody (donkey α-goat HRP, Promega, 1:10,000). For His-tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti- mouse IgG antibody (1:1,000, #S3721, Promega).
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: anti-mouse-IgG (Promega, W4028, 1:2500) and anti-rabbit-IgG (Promega ...
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bioRxiv - Biophysics 2024Quote: ... we also performed a control experiment by blocking the HaloTag with HaloTag Biotin Ligand (Promega). Bacteria in 100 μL sucrose buffer were first incubated with 2.5 μM HaloTag Biotin Ligand (stock stored at -20 °C in DMSO ...
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Microbiology 2020Quote: ... 85 ul of 10% SDS solution and 40 ul of Proteinase K (15mg/ml, MC500B Promega) were added and samples were incubated for 30 minutes at 60° C ...
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bioRxiv - Microbiology 2022Quote: ... 200 mL of the cell suspension was treated overnight with 80 μg/mL proteinase K (Promega) in 1x proteinase K digestion buffer (10mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... proteinase K digestions were stopped by 10 mM phenylmethanesulfonyl fluoride (final concentration) (Protease Inhibitor Cocktail, Promega) on ice for 20 min ...
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bioRxiv - Genomics 2023Quote: ... 500 μl of CTAB lysis buffer and 10 μl of Proteinase K (20 mg/ml; Promega) were added to the sample and incubated at 65ºC for 90 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Cellular proteins were then degraded with 20 µl of 20 mg/mL Proteinase K Solution (Promega) and incubation at 56°C for 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a secondary antibody (Gpx4 1:10000 or 4HNE 1:10000, W4011, Promega and Gapdh 1:10000 or ActB 1:5000, W4021, Promega), diluted in 10% TBS blocking buffer (Licor ...
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bioRxiv - Cell Biology 2023Quote: Cells grown under confluency were incubated in CM+bFGF media containing 1μM cell permeable Janelia Fluor® 549 HaloTag ligand complex (Promega, GA1110) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... transfected with luciferase reporter constructs or containing the relevant promoter regions using FuGENE HD Transfection Reagent Complex (Promega, Madison, Wisconsin, USA). pCMV-NFIA1.1 (a kind gift from Richard Gronostajski ...
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bioRxiv - Developmental Biology 2021Quote: ... and HRP-labelled secondary antibodies (RRID:AB_430833; RRID:AB_430834; Promega; 1:2,000) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... Appropriate HRP-conjugated secondary antibodies were used (1:10000 Promega). Chemiluminescent signals were detected and digitally analyzed ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies include anti-NLuc rabbit polyclonal (1:6,000) (Promega), anti-mCherry rabbit polyclonal (1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... HRP conjugated secondary antibodies were purchased from Promega (1:5000) or Novus (1:5000).
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bioRxiv - Cell Biology 2022Quote: ... Anti-Halo antibody was purchased from Promega (G9211; 1:1000). Anti-actin-HRP antibody (C4 ...
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bioRxiv - Biophysics 2021Quote: ... Biotin-containing handles were made with a PCR on pBluescript SK+ (stratagene) with GoTaqG2 (Promega, M7845), in the presence of 1/5 biotin-16-dUTP (JenaBioscience ...
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bioRxiv - Neuroscience 2024Quote: Anti-HaloTag primary antibody (rabbit, 1 mg/ml, dilution 1:500; catalog no. G928A, Promega) and iFluor 647-conjugated anti-rabbit secondary antibody (goat ...
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bioRxiv - Cancer Biology 2021Quote: ... containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega). Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 5.5μL of a mix containing 5% SDS and 0.25M EDTA and deproteinized with proteinase K (Promega) for 1hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... containing Proteinase K and target regions were amplified by PCR using the GoTaq G2 PCR mastermix (Promega). Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Genetics 2023Quote: ... Additional 140 uL of lysis buffer and 40uL of proteinase K (20mg/ml, Promega, Cat. No. MC5005) were added ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We extracted genomic DNA from tail tissue using proteinase K digestion followed by the Maxwell RSC (Promega) DNA extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... The A7r5 cells and iPSC-SMCs (from 31.3 iPSC clone) were transfected respectively using FuGENE HD Transfection Reagent Complex (Promega, Madison, Wisconsin, USA) and Amaxa Basic Nucleofector kit for primary mammalian SMCs (LONZA ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μl of each reaction was spotted onto a streptavidin-coated SAM2 Biotin Capture Membrane (Promega, #TB547). The membrane was air-dried ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were 1:5,000 HRP-conjugated anti-rabbit (W4011; Promega) or mouse (W4021 ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...