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Citations for Promega :
51 - 100 of 2997 citations for Vitamin K epoxide reductase complex subunit 1 VKORC1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Transcend™ Biotin-Lysyl-tRNA (Promega, Cat.L5061), RNasin® Ribonuclease Inhibitors (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products ...
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bioRxiv - Biochemistry 2020Quote: ... bacmid DNA containing all four subunits was transfected into Sf9 cells using FuGENE® HD (Promega) per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 0.25 mg/mL Proteinase K (Promega V3021) was applied to the leaflet powder as described in Wales et al ...
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bioRxiv - Zoology 2023Quote: ... and 3% 20 mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
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bioRxiv - Biochemistry 2023Quote: ... 1μl of Protease K (Promega, 20 mg/ml) was added and digested for 30 mins at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... PA14pvdF supernatant was incubated with Proteinase K (Promega) (working concentration ...
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bioRxiv - Zoology 2024Quote: ... and 3% 20 mg/ml Proteinase K (Promega). This method removed cellular material while preserving spicules and the spongin network (if present) ...
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bioRxiv - Developmental Biology 2020Quote: ... HaloTag® PEG-Biotin Ligand (Promega Corporation, G8591) was added to pre-cleared 6His-Halo-MBP PRDM9 to 1 μM final concentration and the mixture was incubated 2 hrs at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM HaloTag PEG-biotin ligand (G859A; Promega) or an equivalent amount of DMSO was added ...
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bioRxiv - Genomics 2024Quote: ... We amplified the Cytochrome c oxidase subunit I (COI) for individual abdomen DNA using the GoTaq (Promega) protocol ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Microbiology 2020Quote: ... and 20 μl Protease K (Promega, Madison, WI USA). The sample was incubated for 30 min at 56°C ...
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bioRxiv - Genomics 2022Quote: ... 15 μL of 20 mg/mL Proteinase K (Promega) was then added to the mixture and incubated at 65°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... We identified a common tracer probe (K-4, Promega), suitable for all mutants ...
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bioRxiv - Systems Biology 2022Quote: ... and 40 μl of Proteinase K (Promega, Madison, USA) were vortexed and heated at 95°C for 5 min ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5ul “home-made” Tn5 transposase adapter complex and 0.4mg/ml digitonin (Promega, #G9441) at 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... SAM2® Biotin-Capture Membrane was obtained from Promega, Wisconsin ...
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bioRxiv - Immunology 2022Quote: ... The constructs encoding EROS/CYBC1 or gp91phox/CYBB fused to the reporter subunits were custom made by Promega and consist of the following ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Genomics 2022Quote: ... Single nuclei were sorted into 384 well plates containing 1 µl M-Digestion Buffer containing proteinase K and ~0.05 pg Lambda DNA isolated from dcm+ E.Coli (Promega #D1501).
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bioRxiv - Genetics 2023Quote: ... embryos were rehydrated and treated with Proteinase K (Promega; V3021). Following re-fixation and prehybridization ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Cell Biology 2024Quote: ... Aliquots were then transferred to SAM2 biotin capture membrane (Promega), which was washed ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% SDS with 100μg/μl proteinase K (Promega, Madison, WI, USA), at 65°C for 30min.
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bioRxiv - Genomics 2021Quote: ... washed three times with PBS and digested with Proteinase K (Promega) at a final concentration of 10 ug/mL for 3 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinks were reversed by addition of 2 uL Proteinase K (Promega) and incubation at 65 °C for 12-16 hours in a Thermomixer ...
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bioRxiv - Molecular Biology 2024Quote: ... with one being treated with 50 μg/mL proteinase K (Promega) for 20 min on ice ...
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bioRxiv - Genomics 2023Quote: ... The reaction was stopped with the addition of 150 µL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and 2 µL Proteinase K (Promega, Cat # MC5005), then incubated at 65 °C overnight to reverse crosslinks ...
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bioRxiv - Genomics 2023Quote: ... The reaction was stopped with the addition of 150 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and 2 μL Proteinase K (Promega, Cat # MC5005), then incubated at 65 °C overnight to reverse crosslinks ...
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bioRxiv - Plant Biology 2021Quote: ... We added 300μl of Proteinase K Buffer 2X (200 mM Tris-HCl pH7.5; 100 mM NaCl; 20 mM EDTA; RNasin PROMEGA 20 U/μl; 0.04 mg/ml Proteinase K) and incubated for 1 h at 55 °C ...
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bioRxiv - Physiology 2022Quote: ... 1.15 μg of purified AQP2 or AQP2 S256A COOH-tail peptide were mixed with either RSK (16 nmol) or PKA catalytic subunit (5.6 nmol) (Promega, Madison, WI) in a total volume of 12 μl reaction buffer containing 40 mM Tris pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then treated with 10 µg/ml Proteinase K (Promega V3021) in PTw for 30min ...
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bioRxiv - Genomics 2023Quote: ... 20 μl of Proteinase K (Promega Wizard HMW DNA Extraction Kit A2920) was added and the samples mixed by inverting 10 times and incubated 15 minutes at 56°C followed by chilling on ice for 1 minute ...
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bioRxiv - Microbiology 2024Quote: ... proteinase K (50µg/ml) and 0.5% tween 20 solution] (Promega corporation, USA) for 2 hour at 55°C after vortexing thoroughly and placed in a heat block (DAIHA Scientific ...
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bioRxiv - Biophysics 2020Quote: ... antibodies followed by a 1-hour incubation with the corresponding HRP-conjugated secondary antibodies (1:2000, Promega) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
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bioRxiv - Cell Biology 2020Quote: ... The medium was then replaced with fresh medium in the incubator and protein release was induced by biotin addition (100 µM final) and following Furmazin (Promega, 1/500) addition ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used are anti-HaloTag monoclonal antibody (Promega, G921A, 1:1000), and polyclonal rabbit antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-oligo d(T) (Promega, #Z5261, 0.2 μmol/L for mRNA FISH); Diethyl Pyrocarbonate (DEPC)-treated water (Invitrogen™ ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used: ßgal (1:100, Promega), pMad (1:800 ...
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bioRxiv - Genomics 2021Quote: ... Sorted cells were then unfixed overnight in 50 μg/mL proteinase K (Promega) plus 150 mM NaCl at 65°C ...
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bioRxiv - Genetics 2023Quote: ... and 0.45% Tween-20) with Proteinase K (20mg/ml, Promega, Cat. No. MC5005) added just before use ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting pellet was resuspended and incubated in 2.5mL proteinase K (Promega) at 50ug/mL in 0.5%
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bioRxiv - Immunology 2020Quote: ... The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega) and following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 200 nM of biotinylated Arr3_ýC·IP6 complex was immobilized on 250 µl Streptavidin magnetic beads (Promega, Cat No: Z5482) and incubated with 100 μl of a phage library E (Miller et al. ...
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bioRxiv - Biochemistry 2020Quote: ... the biotin-streptavidin roadblock was prepared by adding streptavidin monomer (Promega, Madison, WI) to 100 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads-biotin-probes-RNA adducts were captured by magnets (Promega, Madison, WI, USA) and washed five times with the Wash buffer (2X SSC ...