Labshake search
Citations for Promega :
101 - 150 of 159 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... and bioluminescence activity of each well was measured using D-luciferin (Luciferase Assay System, Promega) and Varioskan™ microplate reader (Thermo Scientific) ...
-
bioRxiv - Developmental Biology 2022Quote: ... LUC activity was evaluated two days after agroinfiltration in leaves sprayed with D-Luciferin (Promega) using the NEWTON 7.0 CCD imaging system (Vilber) ...
-
Hepatic iNKT cells facilitate colorectal cancer metastasis by inducing a fibrotic niche in the liverbioRxiv - Immunology 2024Quote: ... 20 minutes after intraperitoneal injection (i.p.) of 150 mg/kg D-Luciferin in PBS (Promega). The reagents and equipment used for ex vivo bioluminescence are listed in table S1 and table S2.
-
bioRxiv - Microbiology 2021Quote: ... and was reverse transcribed into cDNAs using oligo-d(T) and M-MLV reverse transcriptase (Promega). Real-time PCR was performed with the specific primers listed in Table 2 using the LightCycler 480 (Roche ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fifty microliters of D-luciferin (30 mg/mL in saline) was injected subcutaneously in mice (Promega). Twenty minutes after the injection ...
-
bioRxiv - Microbiology 2023Quote: ... Then 50 µL of lysates were mixed to 50 µl D-luciferine (Luciferase assay system, Promega) and the Firefly luciferase activity was determined for each cell lysate with an Infinite 200 Pro (Tecan ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice received intraperitoneal injection of 50 μl 30 mg/ml d-luciferin (in PBS) (E1603, Promega). Continuous administration of isofluorane gas was provided to maintain anaesthesia of animals during imaging ...
-
bioRxiv - Molecular Biology 2024Quote: ... mice received an intraperitoneal injection of 150 µg g-1 of D-luciferin (Promega, Charbonnières France), and were then anesthetized (isoflurane 4 % for induction and 1.5 % for maintenance ...
-
bioRxiv - Immunology 2024Quote: ... TNF-α (human; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). For THP-1 viability assays ...
-
bioRxiv - Plant Biology 2021Quote: ... For multiple day measurements plants were sprayed daily with 1 mM D-luciferin (Promega, Fitchburg, Wl, USA) at 10 am ...
-
bioRxiv - Synthetic Biology 2021Quote: 1μl of 50μM oligo d(T)20 (IDT) and 1μl of 10mM dNTP mix (Promega cat #U1240) were combined with 1 μg of RNA before adding dd H20 up to a final volume of 13μl ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 μg of dsRNA was transfected into 1x106 D-Mel cells using Transfast transfection reagent (#E2431, Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice received an intraperitoneal injection of 100 µl D-luciferin (stock of 40 mg/mL, Promega, E160). Mice were allowed to move freely for five minutes post injection prior to initiating anesthesia via isoflurane ...
-
bioRxiv - Biochemistry 2024Quote: ... Mice were anaesthetized by vaporized isoflurane and injected subcutaneously (s.c.) with D-luciferin (2.5 mg/mouse Promega) in 100 μl PBS vehicle ...
-
bioRxiv - Cancer Biology 2024Quote: ... mice were anesthetized with isoflurane and injected intraperitoneally with D-luciferin potassium salt (Promega, Madison, WI, USA). Ten to fifteen min after injection ...
-
bioRxiv - Cell Biology 2024Quote: ... Tumor growth was monitored using bioluminescence imaging by injecting D-luciferin (150 mg/kg i.p.; Promega, Madison, WI) and subsequently imaging mice on an IVIS Spectrum Imaging System (PerkinElmer ...
-
bioRxiv - Cancer Biology 2024Quote: ... Animals were injected with an intraperitoneal dose of 100 mg/kg D-luciferin (Promega, Charbonnières-les-Bains, France) and after five minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tumor growth was monitored using bioluminescence imaging by injecting D-luciferin (150 mg/kg i.p.; Promega, Madison, WI) and subsequently imaging mice on an IVIS Spectrum Imaging System (PerkinElmer ...
-
bioRxiv - Cell Biology 2020Quote: ... and 50µl of 0.1mM D-luciferin K+ salt before measuring luminescence in a GloMax® multi microplate reader (Promega).
-
bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The half maximal inhibitory concentrations (IC50 ...
-
bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
-
bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
-
bioRxiv - Cancer Biology 2022Quote: ... Leukaemia propagation was monitored by bioluminescence using an IVIS imaging system (Caliper) following intraperitoneal (i.p.) injection of 150mg/kg D-luciferin (Promega). For LNPs treatment of adult RG mice ...
-
bioRxiv - Molecular Biology 2022Quote: ... selected Δku80 and Δku80_Δpks1 strains were plated on Sabouraud and YPD agar paltes supplemented with 0.4 mM of d-luciferin potassium salt (Promega) to confirm the expression of a functional luciferase by bioluminescence imaging using ChemiDoc MP (Bio-Rad).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell viability was assessed in uninfected PBMC incubated with each PROTAC under the same conditions (1.0 µM, 4 d) using the CellTiter-Blue assay (Promega).
-
bioRxiv - Microbiology 2023Quote: ... Imaging was performed 3 min after intraperitoneal (i.p.) injection of 150 mg/kg D-Luciferin (Beetle Luciferin Potassium Salt, Promega) in the IVIS® Spectrum In Vivo Imaging System under 2.5% isoflurane inhalation anesthesia using 15 min exposure ...
-
bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5 and 6 d using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (G3582; Promega, Madison, WI). Cells were incubated with the MTS reagent 3-4 h ...
-
bioRxiv - Cancer Biology 2020Quote: ... Leukemia burden was assessed using non-invasive bioluminescence imaging by injecting mice intraperitoneally with 75 mg/kg d-Luciferin (Promega), anesthetizing them with 2–3% isoflurane ...
-
bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase expression was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). Percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
-
bioRxiv - Microbiology 2022Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
-
bioRxiv - Microbiology 2020Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by a DNase I treatment and a subsequent reverse transcription with oligo d[T]18 using the GoScript RT kit (Promega). One-step RT-qPCR was performed using the SYBR FAST Mix optimized for LightCycler 480 (KAPA ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 40,000 cells per well were seeded into poly-D-lysine-coated 96-well plates (Brand, 781965) and the Halo-ligand was added (1:2,000; Promega, G980A). For each transfection ...
-
bioRxiv - Biochemistry 2022Quote: ... RNA was isolated 24 hours after transfection and the pattern of alternative splicing was assessed by RT (with oligo-d(T) / random hexamer) and AMV Reverse Transcriptase (Promega) and PCR with GoTaq (Promega ...
-
bioRxiv - Neuroscience 2023Quote: ... luminescence photons were collected by accumulating the image for 60 s in the presence of 2 mM D-luciferin (Promega), and the luminescence signal was measured from the same varicosities as the corresponding fluorescence image ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein for the 4-methylumbelliferyl-b-D-glucuronide (MUG) and luciferase (LUC) assays was extracted in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40 mL of protein extract to 100 mL of MUG assay buffer [2 mM MUG ...
-
bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Bioluminescent imaging was performed to verify disseminated tumor establishment in mice by injecting D-luciferin subcutaneously at 75 mg/kg (Promega) and imaged with the IVIS Spectrum Imaging System (Perkin Elmer) ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were then plated onto 35 mm glass coverslips pre-coated with poly-D-lysine (100 g/mL) and transfected with 300 ng of each plasmid using FuGene transfection reagent (Promega) or Lipofectamine 2000 (ThermoFisher) ...
-
bioRxiv - Microbiology 2024Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using d-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of nanobody normalized to luciferase readout in the absence of nanobody ...
-
bioRxiv - Immunology 2024Quote: ... Supernatants were then assayed for IL-1β (human; DuoSet; R&D) and relative LDH levels (as a surrogate for pyroptosis) using a CytoTox 96 Kit (Promega). For the THP-1 LPS signaling assays ...
-
bioRxiv - Immunology 2024Quote: ... Supernatants were then transferred to a 96-well plate for storage and assayed for IL-1β (human; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). Once supernatants were removed ...
-
bioRxiv - Immunology 2024Quote: ... Supernatants are then transferred to a fresh 96-well plate for storage and assayed for IL-1β (canine, monkey, porcine, rabbit or rat; DuoSet; R&D) and relative LDH levels using a CytoTox 96 Kit (Promega). Once supernatants are removed ...
-
bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
-
bioRxiv - Cell Biology 2022Quote: The number of viable cells inside HTM hydrogels subjected to the different treatments for 10 d was measured with the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (MTS; Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
-
bioRxiv - Systems Biology 2023Quote: ... and 22 days post-surgery on a Lago X (Spectral Instruments Imaging) after isoflurane anesthesia and intraperitoneal administration of 150 µg D-luciferin (Promega, E1605) per gram body weight to monitor bioluminescence ...
-
bioRxiv - Biochemistry 2024Quote: HEK293T cells seeded in 12-wells plates coated with poly-D-lysine were transfected with 500 ng of empty vector pHTN HaloTag CMV-neo (Promega G7721) or pCEFL-HaloTag-P-Rex1 constructs and co-transfected with 500 ng of SRE-firefly luciferase and 50 ng of Renilla luciferase plasmids ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40,000 cells per well were seeded into white poly-D-lysine-coated 96-well plates (Brand, 781965) in presence of Halo-ligand (Promega, G980A) at a ratio of 1:2,000 ...