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Citations for Promega :
51 - 100 of 159 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and real-time polymerase chain reaction (RT-PCR) conducted using the Techne Prime Pro thermal cycler with GoTaq qPCR Master Mix (Promega). RT-qPCR reactions were carried out in 10 μL volumes containing 3.5 mM MgCl2 ...
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bioRxiv - Bioengineering 2020Quote: ... Luminescence from the activity of luciferase was measured 48 h post-infection using a TECAN infinite M200 pro plate reader after applying Bright-Glo reagent (Promega) on cells.
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bioRxiv - Genetics 2021Quote: ... all clippings from both hands and feet were used to extract genomic DNA with DNA IQ Casework Pro Kit (#AS1240, Promega) and Tissue and Hair Extraction Kit (#DC6740 ...
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bioRxiv - Microbiology 2024Quote: ... and luminescence from the activity of luciferase was measured at 48 h post-infection using a Tecan Infinite M200 Pro plate reader after applying Bright-Glo reagent (Promega) to the cells.
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bioRxiv - Neuroscience 2023Quote: ... and luciferase reported activity was measured with an Infinite M200 Pro Microplate Reader luminometer using the Dual-Luciferase assay system (Promega). Background luminosity was subtracted from un-transfected cells and luciferase readings were normalized by dividing the reported gene firefly luciferase by the reference Renilla luciferase activity ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with 2% 1-thioglycerol using a Bio-Gen PRO200 homogenizer (PRO Scientific, Oxford, USA) and then extracted RNA using the Maxwell RSC simplyRNA Tissue Kit (Promega). We quantified extracts using a Quant-IT RNA Assay Kit ...
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bioRxiv - Developmental Biology 2020Quote: His-P150-CC1 (Courtois et al., 2012) was expressed in and purified from BL21(DE3)pLysS competent cells (Promega) as previously described (Courtois et al. ...
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bioRxiv - Microbiology 2021Quote: ... Restricted fragments to be cloned were ligated into the EcoRI and SacI restricted pBAD/His using T4 Ligase (Promega) before being heat shock-transformed into chemically competent E ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... D-luciferin (beetle) monosodium salt (#E464X, Promega, Walldorf, Germany), Pluronic® PE 10500 (#500053867 ...
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bioRxiv - Neuroscience 2021Quote: ... after which D-luciferin-specific Quantilum Recombinant Luciferase (Promega) was added to a final concentration of 25 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM isopropyl β-d-1-thiogalactopyranoside (IPTG; Promega) was added to each RNAi well for induction of dsRNA synthesis and plates were incubated for 3-4 hours at 30°C and 155 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μg/ml D-luciferin (Promega, Madison, WI, USA) was added before bioluminescence quantification using a Victor luminometer (PerkinElmer ...
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bioRxiv - Immunology 2024Quote: ... 2-3 minutes after 150mg/kg D-luciferin (ProMega) intraperitoneal administration to allow for circulation ...
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bioRxiv - Plant Biology 2024Quote: ... leaves were sprayed with 1 mM D-luciferin (Promega) and incubated in dark for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Biochemistry 2024Quote: ... samples transferred to a fresh plate containing an equal volume of CETSA detection reagent – assay buffer supplemented with 100 nM of purified LgBiT-His protein and 0.5X furimazine (Nano-Glo® luciferase assay system, Promega). Samples were then incubated at room temperature for 30 minutes before transfer to a 384-well white plate (Greiner-One ...
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bioRxiv - Physiology 2020Quote: ... (15mg/kg) with D-luciferin potassium salt (Promega, Madison, WI) dissolved in sterile phosphate buffered saline (30mg/10mL ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then 150 mg/kg D-luciferin (VivoGlo, Promega, Madison, WI) was injected intraperitoneally ...
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bioRxiv - Cancer Biology 2020Quote: ... Mice were intraperitoneally injected with D-luciferin (150mg/kg) (Promega, USA) and anesthetized with pentobarbital sodium ...
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bioRxiv - Physiology 2021Quote: ... with D-luciferin potassium salt (15 mg/kg, Promega, Madison, WI) in sterile phosphate-buffered saline ...
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bioRxiv - Molecular Biology 2022Quote: ... then supplied with 0.4 μl of 100 mM D-luciferin (Promega) per well ...
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bioRxiv - Microbiology 2024Quote: ... Correct protein size of 41.4 kDa for recEmTDH and 40.3 kDa for recMmTDH was confirmed by western blot using a mouse monoclonal anti-His tag antibody and an anti-mouse IgG (h+l) ap conjugate as secondary antibody (Promega, Dübendorf, Switzerland). Finally ...
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bioRxiv - Evolutionary Biology 2024Quote: ... diameter 35 mm) were transfected with 1□µg of each plasmid DNA (pIZ/V5-His having Hmmasc) using FuGENE HD (Promega, WI, USA), as described in Katsuma et al ...
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bioRxiv - Cancer Biology 2020Quote: ... pT7-IRES His-T7-HCF-1VIC was in vitro transcribed/translated using the TnT Quick Coupled Transcription/Translation System (Promega, Madison, Wisconsin, L1171). Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001) ...
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bioRxiv - Microbiology 2021Quote: ... The linearized vector and the slpMh-6x His fragment were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation according to Gibson et al ...
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bioRxiv - Immunology 2021Quote: ... 1 mM L-Glutamin and 250 μM Luziferin D (Promega, Madison, USA). Cells were incubated 12-16 hours in a humidified incubator at 37°C and 5% CO2 to adhere to the bottom of the plate ...
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bioRxiv - Plant Biology 2021Quote: ... Plants were pre-sprayed with 1mM D-luciferin (Promega, Fitchburg, Wl, USA) one day before imaging to deplete accumulated LUC (de Ruijter ...
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bioRxiv - Biochemistry 2021Quote: ... DNA modification enzymes and isopropyl β-D-thiogalactoside (IPTG) were from Promega. Oligonucleotides and molecular weight standards for native gel electrophoresis were supplied by Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-oligo d(T) (Promega, #Z5261, 0.2 μmol/L for mRNA FISH); Diethyl Pyrocarbonate (DEPC)-treated water (Invitrogen™ ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were injected with 150 mg/kg body mass D-luciferin (Promega) via intraperitoneal injection ...
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bioRxiv - Genomics 2020Quote: ... All of these sequences were synthesized as gBlocks® Gene Fragments (Integrated DNA Technologies, Coralville, IA, USA) and cloned into pGL3::PRO (Promega Corporation, Madison, WI, USA) downstream of the luciferase gene ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170, Promega Co., Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... injection of 150 mg/kg D-luciferin (Beetle Luciferin Potassium Salt, Promega, USA). Images were analyzed using LivingImage v4.3.1 software (PerkinElmer ...
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bioRxiv - Cancer Biology 2024Quote: ... following intraperitoneal injections of 150 mg/kg D-luciferin (Promega, Alexandria, NSW, Australia) and under anaesthesia with isoflurane ...
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bioRxiv - Cell Biology 2024Quote: ... the cell population was perfused with 25 µM beetle D-luciferin (Promega, E1601). Within 2–3 min ...
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bioRxiv - Plant Biology 2024Quote: ... After 3 days the leaves were sprayed with D-lucifernin (25mg/L) (Promega) and LUC activity was evaluated using the NEWTON 7.0 CCD imagin system (Vilber) ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant Halo-His-gene was expressed using a 30 μL TNT®SP6 High-Yield Wheat Germ Protein Expression System (Promega, USA, Catalog No L3261). Expression levels were determined by SDS-PAGE and western blot ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which growth media was removed and 20 µl of 4% D-luciferin (Promega) in CO2 independent medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: iPSCs were exposed to 0.3 mg/l D-luciferin (Promega, Benelux, Leiden, The Nederlands). Images were obtained immediately after D-luciferin application with the IVIS® Spectrum (Caliper Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... mice were injected subcutaneously with 75 mg/kg D-luciferin potassium salt (Promega E1605) in sterile PBS and anesthetized with 2% isoflurane in medical air ...
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bioRxiv - Cancer Biology 2020Quote: ... mice were injected intraperitoneally with 150 mg/kg body weight of d-luciferin (Promega) and 10 minutes later the mice were imaged using a Xenogen IVIS Lumina system with Living Image version 3.0 software (Caliper Life Sciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... renilla and NanoLuc luciferases were measured in a Berthold luminometer using D-luciferin (Promega), collantrazine (Biotium ...
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bioRxiv - Microbiology 2024Quote: ... Mice were intraperitoneally injected with 200 μl of 40 mg/ml D-luciferin (Promega) in PBS and anesthetized with isoflurane ...
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bioRxiv - Microbiology 2021Quote: ... The lungs were incubated with 1 mg/mL of D-luciferin (Promega, Madison, WI, USA) for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Following this incubation luciferase activity was measured in the presence of D-luciferin substrate (Promega) using a Centro LB960 plate luminometer (Berthold) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was assayed 6 d after compound addition with the CellTiter-Glo reagent (Promega). Luminescence well values were normalized to DMSO-treated wells by subtracting per-plate average DMSO log2-luminescence values from the log2-luminescence values of each treatment well.
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bioRxiv - Cancer Biology 2022Quote: ... ATP levels were measured using the CellTiter-Glo 3-D Reagent (Promega, catalog no. G9681) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... for Calu-3 cells and CellTiter-Glo 3-D Cell Viability Assay Kit (Promega, Germany) for hBAECs according to the manufacturer’s protocol ...