Labshake search
Citations for Promega :
1401 - 1435 of 1435 citations for 6 BENZOFURAN 2 YL 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
-
bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: 3’ UTR fragments of miRNA target genes (for primer sequences see Table S2) were cloned into the XhoI/NotI sites downstream of the synthetic Renilla luciferase gene of psiCHECK-2 vector (Promega; CatNo. C8021). Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... 16.5 μg attB-plasmid library + 3 μg pCAG-NLS-Bxb1 + 2 μg pMAX-GFP + 2150 μL serum-free DMEM + 80 μL FugeneHD (Promega cat# E2311), then incubated at room temperature for 15 min and added dropwise to cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... Probes are synthetized from a PCR product or a linearized plasmid (see Table 2) with the T7 or SP6 RNA polymerase (Promega, P4074 or P4084) in presence of DIG-UTP nucleotides ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 to 2 µg of RNA was used for DNase treatment as per the protocol provided by the manufacturer (Promega, Madison, Wisconsin, USA). This was followed by cDNA synthesis using reverse transcriptase kits from Applied Biosystems (High capacity cDNA synthesis kit ...
-
bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
-
bioRxiv - Biochemistry 2019Quote: ... The beads were again washed with 50 mM ammonium bicarbonate buffer and resuspended in 100 µl of fresh 50 mM ammonium bicarbonate buffer and treated with 2 µg of sequencing grade modified trypsin (Promega; Product code V5113) at 37 oC overnight ...
-
bioRxiv - Molecular Biology 2020Quote: ... The RNA samples (2 μg) were used for the synthesis of cDNA by reverse transcription using GoScript ™ Reverse Transcriptase (Promega, Madison, USA). RT-PCR was performed with a CFX96 Touch ™ Real-Time PCR Detection System (Bio-Rad Laboratories) ...
-
bioRxiv - Cell Biology 2021Quote: Hela cells were transfected with 2 µg of HaloTag® fusion construct and 20 ng NanoLuc® fusion construct using Fugene® HD (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 x 105 U2OS cells were transfected with 2 µg of sgRNA and Cas9 expressing plasmid using Fugene HD reagent (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2022Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used in this study were as follows ...
-
bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
-
bioRxiv - Physiology 2019Quote: ... The wounded area and its surrounding area (2 x 2 cm) were immediately dissected out and RNA extraction was conducted using Maxwell® RSC simplyRNA Tissue Kit (AS1340, Promega, Madison WI) following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... The protein solution was further diluted down to less than 2 M urea with 50 mM Tris-Cl (pH 8) and incubated with 2 μg of modified trypsin (w/w, 1∶50) (Promega, Madison, WI, USA) at 37°C overnight ...
-
bioRxiv - Systems Biology 2024Quote: ... the samples were diluted with 50 mM HEPES (pH 8.5) to reduce urea concentration to 2 M and further digested overnight with trypsin (Promega, 1:50 w/w) at RT ...
-
bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
-
bioRxiv - Bioengineering 2023Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used to detect the WK-521 strain were as follows ...
-
bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
-
bioRxiv - Plant Biology 2023Quote: ... and 2 µg of RNA were used for reverse transcription into complementary DNA (cDNA) using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: Affinity purified proteins retained on beads were resuspended in 100 µL of NH4HCO3 at 25 mM containing 2 µg of Trypsin/Lys-C mix (Mass Spec Grade, Promega, Madison, WI, USA). Digestion was performed under agitation at 37°C during 4h ...
-
bioRxiv - Microbiology 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, Cat. no. V5117) directly on top of the column and incubating overnight at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... beads were equilibrated in 50 mM of ammonium bicarbonate with 2 washes followed by on-bead digestion with trypsin (800 ng; Promega cat. nr. V5280) overnight at 37 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... membranes were washed 3 times for 5 minutes in TBST or TBST-XL for mucin-2 and incubated with anti-mouse IgG HRP Conjugate (1:10000, Promega, Cat no. W4021) or anti-rabbit IgG HRP Conjugate (1:10000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... After two low stringency washes for 5 minutes each with 2× saline sodium citrate/ 0.1% sodium dodecyl sulfate buffer (2x SSC buffer; Promega Co., Madison, WI, Catalog# V4261) and a high stringency wash for 2 min with 1x SSC buffer ...
-
bioRxiv - Microbiology 2020Quote: ... faginata MAT1-1 isolate using a CTAB-chloroform DNA extraction method (van Diepen et al. 2017) and a MAT1-2 isolate using a Wizard® kit (Promega, Madison, WI, USA) and suspended in 75 µl Tris-EDTA (TE ...
-
bioRxiv - Microbiology 2021Quote: ... cells were resuspended at 2×106 cells/ml in culture media containing Nano-Glo® Endurazine Live Cell Substrate™ (Promega Inc, WI, USA # N2571) and DrkBiT (Promega Inc ...
-
bioRxiv - Cell Biology 2021Quote: ... The samples were diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and the proteins were digested with 4 µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073 ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900 μL of 0.35 M KCl (10 mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
-
bioRxiv - Biophysics 2022Quote: ... spheroplast/sucrose buffer suspension was added to 500 μL warm (42 °C) agarose solution (low melting point agarose, V2831 Promega, 2% w/v in sucrose buffer) using a cut pipette tip ...
-
bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
-
bioRxiv - Developmental Biology 2024Quote: ... The membranes were washed 3x 10 minutes in TBS-T before being incubated for 2 hours with the secondary anti-mouse-HRP- or anti-rabbit HRP antibody (1:5000, Promega anti-mouse, W4021; anti-rabbit W4011). The membranes were washed in TBS-T for 3x 10 minutes and developed with Western Lightning Plus-ECL reagent (Perkin Elmer NEL104) ...
-
bioRxiv - Genomics 2024Quote: ... pooled PCR fragments > 600 bp in length (300 ng per tube x 2 tubes, each diluted with 100 μl of Promega RQ1 DNase 10x buffer (cat. # M6101)) were lightly digested with 0.2 μl of DNase-I (RQ1 RNase-Free DNase-I ...