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Citations for Promega :
1151 - 1200 of 1435 citations for 6 BENZOFURAN 2 YL 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of pCMV-Sport- Polκ were incubated with 100 µL of TnT® Sp6 Quick Master Mix (Promega) supplemented with 4 μL of 1 mM methionine for 90 min at 30°C ...
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bioRxiv - Neuroscience 2023Quote: ... Urea concentration was lowered to ¡2 M by addition of 100 mM Ammonium Bicarbonate and sequencing-grade modified trypsin 1.5 µg (Promega) was added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: Transient transfection was performed using 1 or 2 µg plasmid DNA and 3.5 or 7 µl FuGENE HD transfection reagent (Promega) per 5x 105 cells ...
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bioRxiv - Biochemistry 2024Quote: ... immune precipitated samples were washed 5 times with lysis buffer and treated with Trypsin Gold (2 μg/ml; Promega) in 20 mM Tris-HCl (pH7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... were supplemented with 1 µL of 1M ammonium bicarbonate (AmBic) and digested with 2 µL of 0.05 µg/µL sequencing grade trypsin (Promega) in 100 mM AmBic ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were further diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and proteins were digested with 50 µg trypsin (Promega) (1/200 ...
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bioRxiv - Physiology 2024Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... One 10-cm plate of HEK-293T cells at 90% confluency was transfected with 6.5 μg of the pBMN-ARHGAP36 (isoform 2)-mCherry mutant library and 4 μg of pCL-ECO using the FuGene HD transfection reagent (Promega). The medium was replaced after 24 hours with DMEM containing 1.8 mM L-glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... were inserted using XhoI and NotI sites in 3’ UTR of Renilla gene in the psiCheck-2 dual luciferase reporter vector (Promega). The reporter in the amount of 25 ng and plasmids with different NORAD variants in the amount of 200 ng were introduced per 30,000 cells in 24-well plates ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior tissue (at least 2 mm away from the outermost edge of the specimen) using the Wizard Genomic DNA Purification Kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
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bioRxiv - Immunology 2022Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction ...
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bioRxiv - Molecular Biology 2019Quote: ... The first strand cDNA was synthesized with 2 μg of purified total RNA using the reverse transcription system (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Inverse PCR reactions were carried out with 2 μL of DNA solution in a final volume of 20 μL using the GoTaq enzyme (Promega). The PCR conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: Protein digestion - for samples processed with reagent 1 and 2 as well as for supernatants (proteomes) MS grade trypsin (Promega) was used at 1:1000 w/w protease:protein ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant 3’ UTR of BRCA1 were constructed into psi-check-2 vector (Promega, Madison, WI, USA). Cells were plated in 24-well plates and the plasmids (psi-check-2-WT ...
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bioRxiv - Microbiology 2019Quote: ... The entire PCR reaction was run on a 2% agarose TAE gel followed by excising the bands and clean-up using Wizard SV Gel Clean-up (Promega). cDNA from WT EEEV and 11337 stocks were used as positive controls during PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... All constructs were separately inserted into the multiple cloning region located downstream of the Renilla translational stop codon within the psiCheck-2 vector (Promega). psiCheck-2 reporters (100 ng ...
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bioRxiv - Plant Biology 2020Quote: ... following the manufacturer’s instructions (eluted into 8 μl of TLE) before A-tailing in a reaction containing 2 μl of GoTaq buffer (Promega), 0.2 μl 10 mM ATP ...
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bioRxiv - Microbiology 2021Quote: ... NF54-HGL parasites were treated with 3× IC50 for two weeks in two independent experiments and cultures were monitored by luminescence readout on the BioTek Synergy 2 Plate reader using ONE-Glo reagent (Promega). Whole-genome sequencing was performed to test for single nucleotide polymorphisms (SNPs ...
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bioRxiv - Biochemistry 2021Quote: ... FN1-GFP together with pRSV40 Renilla (2% of total transfected DNA) were transiently transfected and lysed in Passive Lysis Buffer (Promega). Cell lysates were analyzed by Western blot while Renilla luciferase activity (Glomax luminometer ...
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bioRxiv - Neuroscience 2021Quote: ... of miR-3594-5p was amplified from rat brain cDNA and cloned downstream of the Renilla luciferase reporter gene into the psiCHECK-2 vector (Promega).
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bioRxiv - Plant Biology 2021Quote: ... 2 μg of each purified plasmid (pTnT-SEP3-3FLAG and pTnT- AGIAP1-5MyC) were used as input in a 50 μl TnT reaction incubated at 25 °C for 2 hr (Promega). The reaction solution was then combined with 50 μl IP buffer (PBS supplemented with 0.005 % NP40 and proteinase inhibitors (Roche) ...
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bioRxiv - Biochemistry 2021Quote: ... 55 mM IAA at room temperature for 30 min in the dark and digested at 37°C for 16 h with 2 μg porcine trypsin (sequencing grade, Promega). The resultant tryptic peptides were extracted in 40% ACN/0.1% TFA into fresh Protein LoBind® microtubes ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
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bioRxiv - Microbiology 2020Quote: ... were made by end-labeling one primer of a complementary pair (primers listed in Table 2) using 20 μM Cy5-dCTP and Terminal deoxynucleotidyl Transferase (TdT; Promega). Complementary primers (one labeled with Cy5 and the other unlabeled ...
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bioRxiv - Microbiology 2020Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalized to a mock-treated control to generate a fold induction.
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bioRxiv - Systems Biology 2021Quote: ... Samples were resuspended in 100 µL of 50 mM ammonium bicarbonate and then subjected to proteolysis using either 2 µg of trypsin (Promega), 2 µg of AspN (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... and digested for 2 hours at 37°C with ApaI and SpeI restriction enzymes and ligated with T4 DNA ligase (Promega) overnight at 16° to a double-digested pKNG101 plasmid ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... at room temperature, with 40 μL assay buffer (CO2-independent medium, 10% FBS) supplemented with 2% (v/v) GlosensorTM reagent (Promega). To stimulate endogenous cAMP via β adrenergic-Gs activation ...
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bioRxiv - Plant Biology 2020Quote: ... The concentrations of the resulting cDNAs were adjusted to 10 ng/µL and 2 µL were used for RT-qPCR reactions performed with the GoTaq qPCR Master Mix (Promega) in a final volume of 10 µL ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well and incubated for 2 min or cells were lysed with Cell lysis buffer (Promega E1500) and placed on a rocker for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... injecting 50 µl per well of coelenterazine substrate (Nanolight Technologies, 2 µg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction.
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested for 2 h at 37°C with Lys-C and 16 h at 37°C with Trypsin Gold (both Promega). For TMT 6-plex labeling (Thermo Fisher Scientific) ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Proteins were digested with endoproteinase LysC (Wako Laboratories) for 2 h at 25 °C followed by overnight digest with sequencing-grade trypsin (Promega) at 25 °C (enzyme-to-substrate ratios of 1:50) ...
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bioRxiv - Cancer Biology 2020Quote: A 500bp sequence containing the predicted miR-224 binding site at the 3’ UTRs of ADAM17 or a 500bp sequence containing scrambled sequence cloned into the Xho I/Not I site of psiCHECK-2 vector (Promega) to generate PsiCHECK-2-ADAM17-WT and PsiCHECK-2 -ADAM17-mut vectors ...
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bioRxiv - Cell Biology 2021Quote: ... On-bead digestion was performed by overnight incubation at 37°C with 2 μg of mass spectrometry grade trypsin (Promega). The following morning an additional 2 μg of trypsin was added to each sample and incubated at 37°C for 4-6 hours ...
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bioRxiv - Genomics 2021Quote: ... Cell number was normalized before seeding by measuring ATP levels in a 1:2 dilution series of digested organoids with CellTiter-Glo (Promega). The number of cells matching 10,000 photons (Berthold Technologies ...
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bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37° C and subsequently diluting to 1 M urea with 50 mM NH4HCO3 and finally adding 2% (w/w) trypsin (Promega) for 14 hours at 37° C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended at 2 x 106 cell mL-1 in complete media and HaloTag substrate conjugated to TMR (Promega) was added at a final concentration of 5 μM ...
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bioRxiv - Neuroscience 2022Quote: ... the used PCR primers were flanked with SfiI and NheI sites to allow the insertion in front of hRluc reading frame in psiCheck-2 vector (Promega).
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated in the dark for 2 min and then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Biochemistry 2022Quote: ... before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg /μL sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off ...
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bioRxiv - Microbiology 2022Quote: ... the HSV-1 ICP0 3’UTR was amplified from pICP0(HSV-1) using psiV1ICP0UTRf and psiV1ICP0UTRr primers and inserted into psiCHECK-2 (Promega). psiChHVICP03UTR was constructed by inserting the synthesized ChHV ICP0 3’ UTR into the same position ...
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bioRxiv - Biochemistry 2022Quote: ... 2ml of Sf9 cells at 0.5×106 cells per ml were transfected with 2 µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a ratio of 3:1 transfection reagent to DNA ...