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Citations for Promega :
1351 - 1400 of 2342 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... medium was removed from the cells and replaced by MTS reagent (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI) diluted in PBS ...
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bioRxiv - Microbiology 2021Quote: ... was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega (Madison, WI, USA). Vero E6 cells were seeded in 96-well plates at a density of 10,000 cells/well ...
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bioRxiv - Developmental Biology 2021Quote: ... in media containing 1% FBS and tested for expression of luciferase using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega #E7120). Luciferase expression was measured using a Flexstation 3 plate reader (Molecular Devices) ...
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bioRxiv - Genomics 2021Quote: ... The barcoded pool was sized on a Agilent Tapestation D5000 tape and quantified using QuantiFluor® ONE dsDNA System (Promega, WI, USA) and the molarity calculated.
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bioRxiv - Neuroscience 2023Quote: ... a subset of cells underwent viability testing via MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega, Catalog #G3582) or ATP assay (CellTiter-Glo® Luminescent Cell Viability Assay ...
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bioRxiv - Molecular Biology 2023Quote: ... one of these plasmids is combined with a non-targeted firefly luciferase reporter pGL4-SV40 (Promega; for simplicity referred to as FL) and a Renilla luciferase reporter carrying a Mos cognate sequence complementary to MosIR-expressed dsRNA ...
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bioRxiv - Biochemistry 2023Quote: One-hundred microliters of 50 mM Tris-HCl (pH 8.0) and 500 ng of trypsin/Lys-C mix (Promega, cat. no. V5072) were added to the washed HaloTag ligand plate following the first-generation assay and mixed gently at 37 °C overnight to digest the proteins ...
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bioRxiv - Bioengineering 2023Quote: ... the liquid DMEM culture medium and the hydrogel overlaying the cultured chondrocytes were first removed before introducing 300 µL of CellTiter 96® Aqueous One Solution Cell Proliferation assay (Promega, Australia) working solution ...
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bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured for 1 day in 96-well plates and treated with small molecules for 3 consecutive days prior to incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Microbiology 2024Quote: THP-1 macrophage viability was determined by colorimetric method using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega; G35C) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... were screened in the abdomens and head-thorax sections of mosquitoes by RT-qPCR associated with high-resolution melting curve (Vina-Rodriguez et al 2017) using one step mixes (GoTaq® 1-Step RT-qPCR System, Promega, US). Positive flaviviruses samples were subjected to a nested PCR on a 960 bp fragment of the NsP5 gene following Vazquez et al ...
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bioRxiv - Molecular Biology 2024Quote: One hundred micrograms of urinary proteins from each sample were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2024Quote: Toxicity was evaluated by measuring the lactate dehydrogenase (LDH) level in cell supernatants using the CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit (Promega, G7892) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Cell viability of the inks screening assays and the bioprinted samples was assessed using the 96®AQueous One Solution Cell Proliferation assay (MTS assay, Promega, USA). The supplier protocol was used ...
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bioRxiv - Bioengineering 2024Quote: ... Relative firefly luciferase activity and cell viability were assessed ∼24 h after LNP addition using ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured 72 h after the drug treatment using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Spain). PBS-treated cells were used as control ...
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bioRxiv - Cancer Biology 2024Quote: Cell growth and viability were assessed using the MTS CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, Wisconsin, US) after 72 hours of treatments ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell proliferation was analyzed after 48 hours culture using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The A2780 cell proliferation was analyzed 48h after cultured in CM using CellTiter One Solution Cell Proliferation Assay kit (MTS assay, Promega, Cat# G8461) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Metabolic activity was measured at day 4 with CellTiter 96 (Promega) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... Then 4 µL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Then 4 μL/well of Bright-Glo luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... and overnight protein digestion using 4 μg MS-grade trypsin (Promega) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... by using 4 μL of FuGENE HD transfection reagent (Promega #E2311) according manufactured protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Then 4 μL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 nM dT-primer and 4 U RNase Inhibitor (Promega, N2611). Reverse transcription was performed at 42°C for 90 min after filling up to 10 μl with RT buffer mix for a final concentration of 1x superscript II buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Resin was then washed 4 times with Resin Wash Buffer (Promega). Complexed proteins were eluted in SDS Elution Buffer (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Systems Biology 2020Quote: ... Reduced proteins were alkylated and trypsin-digested for 1-2 hours in trypsin solution (2 mg/ml sequencing grade porcine trypsin (Promega)/100 mM Tris/10 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 mM MgCl2, and 2 mM ATP (for nucleic acid substrates containing RNA, 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 30 min at 37 °C ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl RNasin Plus (Promega, catalog no. N2615), 0.8 µl 100 mM ATP (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... using 2 µg of sequencing grade trypsin (Promega) per fraction 55 ...
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bioRxiv - Biochemistry 2022Quote: ... either 2 µg pGL4.47[luc2P/SIE/Hygro] (Promega) or pGL4.52[luc2P/STAT5RE/Hygro] (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... 2) pRL Renilla luciferase control reporter (Promega, E2241) and 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... pGL4.10 (Luc.2; E665A) was purchased from Promega. The pGL4.10-ITGB1 reporter and a control Renilla luciferase vector were co-transfected into breast cancer cell lines using Lipofectamine 3000 transfection kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 2 uL of random primers (Promega, C1181). This was then incubated at 70 °C for 10 mins ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µl of 5x MTase-Glo Reagent (Promega) was added to wells ...
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bioRxiv - Cancer Biology 2023Quote: ... we used CellTiter Glo 2 assay systems (Promega). Approximately 2.0 × 103 cells/well were seeded in a 96-well plate and incubated in RPMI1640 culture medium over 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL of 100 bp DNA Ladder (Promega) was added to confirm amplicons size and run at 100 V for 45 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with 2 μg trypsin (Promega, v511c), shaking overnight at 700 rpm ...